Hemin-Hemin-chloride-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEHeminCat.No.:HY-19424CASNo.:16009-13-5Synonyms:Heminchloride分⼦式:C₃₄H₃₂ClFeN₄O₄分⼦量:651.94作⽤靶点:Autophagy;Mitophagy;Ferroptosis作⽤通路:Autophagy;Apoptosis储存⽅式:4°C,protectfromlight,storedundernitrogen*Insolvent:-80°C,6months;-20°C,1month(protectfrom

light,storedundernitrogen)溶解性数据体外实验1MNaOH:6.67mg/mL(10.23mM;ultrasonicandadjustpHto12withNaOH)MassSolvent1mg5mg10mgConcentration制备储备液1mM1.5339mL7.6694mL15.3388mL5mM0.3068mL1.5339mL3.0678mL10mM0.1534mL0.7669mL1.5339mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(protectfromlight,storedundernitrogen)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。BIOLOGICALACTIVITY⽣物活性Hemin含铁的卟啉化合物。Hemin⾎红素加氧酶(HO)-1诱导剂。IC50&TargetHemeoxygenase[1]体外研究HeminandPGJ2,usedaspositivecontrols,stronglyincreasebothexpressionandactivityofHMOXafter41/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEand12h,respectively.Indeed,asignificanteffectisfoundof30μMHeminoncellproliferationinallusedcelllinesafter48h,whichisdose-dependent.Hemintreatmentdecreasescellproliferationto62±5%,51±3%,and38±8%inPA-TU-8902,BxPC-3andMiaPaCa-2cancercells,respectively,withp[1].体内研究Followingthei.p.administrationofHemin(100μmol/kg),theHO-1levelintherenalcortexbeginstoincreasegradually.TheHO-1levelreachesitspeak24hafterHeminpreconditioning.HO-1isexpressedmainlyintherenaltubules.TheHO-2levelinthekidneydoesnotchangefollowingHeminpreconditioning[2].PROTOCOLCellAssay[1]Forthecellproliferationassay,cellsareseededinto96well(5-12.5×104cellspermLaccordingtothecellline)andkeptat37°Cand5%CO2.After24h,cellsaretreatedwithstatinsor/andHemin,followedbytheMTTtestasageneralcellproliferationassay[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.AnimalMice[2]Administration[2]Eight-toten-week-oldmaleBABL/cmiceareusedfortheischemia-reperfusion(I/R)experiments.Theanimalsaredividedintofivegroupsasfollows:(1)theshamgroupundergoisolationofthebilateralrenalarterieswithoutclamping;(2)thevehiclegroupreceiveanintraperitoneal(i.p.)injectionof4mL/kgPBSasavehiclecontrol(withIRI);(3)theHemin-preconditionedgroupreceiveHemin,apotentinducerofHO-1,at100μmol/kgi.p.;(4)theHeminplusZnPPgroupreceivezincprotoporphyrinIX,aninhibitorofHO-1activity,at5mg/kgi.p.6hafterreceiving100μmol/kgHemini.p.;and(5)theHeminplusPD98059groupreceivePD98059,aninhibitorofERK1/2activity,at10mg/kgi.p.6hafterreceiving100μmol/kgHemini.p.Bothinhibitorsareadministeredi.p.2hbeforeI/R,whereasHeminwasadministered8hbeforeI/R.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.户使⽤本产品发表的科研⽂献•CellMolImmunol.2022Jan5.•CancerLett.2022Jul19;215831.•FreeRadicBiolMed.2022Nov17;193(Pt2):795-807.•Antioxidants.2021Jun29;10(7):1049.•JInvestDermatol.2021Jul6;S0022-202X(21)01416-0.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].VanovaK,etal.Hemeoxygenaseisnotinvolvedintheanti-proliferativeeffectsofstatinsonpancreaticcancercells.BMCCancer.2016May12;16:309.[2].ChenHH,etal.Hemeoxygenase-1ameliorateskidneyischemia-reperfusioninjuryinmicethroughextracellularsignal-regulatedkinase1/2-enhancedtubularepitheliumproliferation.BiochimBiophysActa.2015Oct;1852(10PtA):2195-201.McePdfHeight2/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemECaution: