Polydatin-Piceid-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEPolydatinCat.No.:HY-N0120ACASNo.:27208-80-6Synonyms:Piceid分⼦式:C₂₀H₂₂O₈分⼦量:390.38作⽤靶点:Autophagy;Apoptosis;Mitophagy作⽤通路:Autophagy;Apoptosis储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:≥29mg/mL(74.29mM)H2O:0.1mg/mL(0.26mM;Needultrasonic)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制备储备液1mM2.5616mL12.8080mL25.6161mL5mM0.5123mL2.5616mL5.1232mL10mM0.2562mL1.2808mL2.5616mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(6.40mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(6.40mM);Clearsolution3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(6.40mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性Polydatin提取⾃从传统中药虎杖根，在多个实验模型中具有抗炎作⽤。Polydatin(Piceid)可抑制G6PD，并诱导氧化和内质⽹应激IC50&TargetNF-κB,Autophagy体外研究Polydatinprotectscerebralcellsfromischemicdamagesviaimprovementofmicrocirculationandinhibitionofplateletaggregation.Inaddition,polydatininhibitsICAM-1expressioninendothelialcellsstimulatedbylipopolysaccharide;italsoattenuatesadhesionbetweenwhitebloodcellsandendothelialcells[1].Polydatininhibitscancercellproliferationandcellcycleprogression[4].PolydatininducesERstress-drivencelldeath[4].体内研究PolydatincouldsignificantlyincreasetheactivityofSODandtheheartrate,attenuatemyocardialpathologicaldamage,decreaseMDAcontent,slightlyincreasearterialpressureandGSH-Pxactivity,reduceintervalsofQRS,QTandST,andlowerFFAcontent[2].PROTOCOLCellAssay[3]TheeffectofpolydatinonmMECviabilityisevaluatedwithanMTTassay.mMECsareincubatedinthepresenceorabsenceofvariousconcentrationsofpolydatin(25,50and100μg/mL)andDEX(100μg/mL)for24h.Next,20μLofMTT(5mg/mL)isaddedtoeachwellandincubatedfor4h.Afterthesupernatantsareremovedandtheformazanisdis¬solvedwith150μLofDMSOineachwell,theopticaldensity(OD)valueismeasuredat570nmonamicroplatereader[3].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.AnimalRats:Ratsaredividedintosixgroupsbyrandomassignmentandtreatedasfollows:innormalgroupandAdministration[2][3]polydatincontrolgroup:ratsareadministratedwithCMC-Naandpolydatin(200μmol/kg)bygavagerespectively,andgivennormalsaline(NS)bytailintravenous(iv)injectionwiththesamevolume;inDOXgroup:ratsareinjectedwithDOXbycaudaveinfor4weeks(3mg/kgperweek),thecumulativedosageis12mg/kgsimilartothatintheresearchofChangetal[2].Mice:Polydatinisdissolvedindimethylsulfoxide(DMSO)anddilutedinDulbecco’smodifiedEagle’smedium(DMEM).SixtyadultfemalepostpartumandlactatingBALB/cmice(6–8weeksold,weighing35–40g)areobtained.Controlgroup(CG):Themicearetreatedwith100μLofPBSasavehiclecontrol.Polydatin2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEgroups:24hafterSaureusinfection,themousemodelofSaureusmastitisisintraperitoneallyadministeredpolydatinat15,30and45mg/kg[3].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.户使⽤本产品发表的科研⽂献•OxidMedCellLongev.2022Mar27;2022:5696686.•JEthnopharmacol.2021Jul31;114479.•NeurotoxRes.20August2022.•MolMedRep.2022Jan;25(1):9.•PlantDirect.2022Sep2;6(9):e442.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].ChengY,etal.Involvementofcelladhesionmoleculesinpolydatinprotectionofbraintissuesfromischemia-reperfusioninjury.BrainRes.2006Sep19;1110(1):193-200.[2].WangHL,etal.SynergisticeffectsofPolydatinandVitaminCinInhibitingCardiotoxicityinducedbyDoxorubicininrats.FundamClinPharmacol.2016Nov28.[Epubaheadofprint][3].JiangKF,etal.PolydatinamelioratesStaphylococcusaureus-inducedmastitisinmiceviainhibitingTLR2-mediatedactivationofthep38MAPK/NF-κBpathway.ActaPharmacolSin.2016Nov28.[Epubaheadofprint][4].LuigiMele,etal.ANewInhibitorofglucose-6-phosphateDehydrogenaseBlocksPentosePhosphatePathwayandSuppressesMalignantProliferationandMetastasisinVivo.Cell