Tangeretin-Tangeritin-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemETangeretinCat.No.:HY-N0133CASNo.:481-53-8Synonyms:Tangeritin;NSC53909;NSC618905分⼦式:C₂₀H₂₀O₇分⼦量:372.37作⽤靶点:Notch;Apoptosis作⽤通路:NeuronalSignaling;StemCell/Wnt;Apoptosis储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:25mg/mL(67.14mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM2.6855mL13.4275mL26.8550mL5mM0.5371mL2.6855mL5.3710mL10mM0.2686mL1.3428mL2.6855mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>90%cornoil1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemESolubility:≥2.5mg/mL(6.71mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性Tangeretin(Tangeritin)，酮类化合物，Notch-1的有效抑制剂，具有抗炎，保护神经等作⽤。IC50&TargetNotch-1体外研究TangeretinenhancedtheradiosensitivityofGCcellsasdemonstratedbyMTTandcolonyformationassays.Tangeretinalsoattenuatedradiation-inducedEMT,invasionandmigrationinGCcells,accompaniedbyadecreaseinNotch-1,Jagged1/2,Hey-1andHes-1expressions.TangeretintriggeredtheupregulationofmiR-410,atumor-suppressivemicroRNA.Furthermore,re-expressionofmiR-410preventedradiation-inducedEMTandcellinvasion[1].体内研究Inthisstudy,weinvestigatedtheinvivoanti-RSVactivityoftangeretinin3-week-oldmaleBALB/cmice.Aplaquereductionassayandfluorescencequantitativepolymerasechainreaction(FQ-PCR)showedthattangeretininhibitedRSVreplicationinthelungofmice[2].PROTOCOLCellAssayTheeffectoftangeretinonRAW264.7cellswasdeterminedusingaMTTassayaspreviouslyreported.(13)Briefly,RAW264.7cells(1×104cells/well)wereseededina96-wellplatefor24handtreatedwithdifferentconcentrationsoftangeretin(6.3–50.0μM)anddimethylsulfoxide(DMSO)(vehiclecontrol,0.01and0.1%)for10or48h.Theabsorbancewasmeasuredat570nmusinganenzymeimmunoassay(EIA)reader(ThermoScientific,Waltham,MA),andcellviability(%)wascalculatedasfollows:[(absorbanceofthetestgroup–absorbanceoftheblankcontrol)/(absorbanceofthecontrolgroup–absorbanceoftheblankcontrol)]×100.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.AnimalAnimaladministration[2]Themiceweremaintainedinanair-conditioned,pathogen-freeroom(temperatureAdministrationof24±2°C,witha12hlight/darkcyclefrom6:00amto6:00pm)withfreeaccesstofoodandwater.Micewererandomlydividedintofivegroups(n=10)asfollows:normal(control),RSV-challenged,andthreetreatmentgroupsadministered25,50,or100mg/kg/daytangeretindissolvedinsaline.ThecontrolandRSV-challengedgroupsreceivedequalvolumesofsaline.Duringtheexperiment,miceinthetreatmentgroupswereintragastricallyadministratedtangeretinfor3daysconsecutivelybeforeRSVstimulation.MicewerelightlyanesthetizedwithdiethyletherandintranasallychallengedwithRSVLongstrain[6.7×106plaque-formingunits(PFU)]onday4aftertangeretintreatment,whilethecontrolgroupwassham-infectedwithanequalvolumeofHEp-2celllysate,whichwascentrifugedunderthesameconditionsastheviralsuspensions.Themicewereweighedduringtheexperimentandsacrificedonday5post-infectionafteranesthetizingthemwithchloralhydrate(Figure1B).Thelungtissueswereremovedandweighed,andthelungindexwascalculatedusingthefollowingformula:lungindex=lungweight/bodyweight.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE户使⽤本产品发表的科研⽂献•BiomedPharmacother.2020Sep;129:110369.•Phytomedicine.5January2022,153928.•JAgricFoodChem.2022Sep5.•JAgricFoodChem.2022Feb9;70(5):1536-1546.•BiochemBiophysResCommun.17September2022.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].ZhangX,etal.Tangeretinenhancesradiosensitivityandinhibitstheradiation-inducedepithelial-mesenchymaltransitionofgastriccancercells.OncolRep.2015Jul;34(1):302-10.[2].XuJJ,etal.TangeretinfromCitrusreticulateInhibitsRespiratorySyncytialVirusReplicationandAssociatedInflammationinVivo.JAgricFoodChem.2015Nov4;63(43):9520-7.[3].HagenlocherY,etal.CitruspeelpolymethoxyflavonesnobiletinandtangeretinsuppressLPS-andIgE-mediatedactivationofhumanintestinalmast