Nimustine-DataSheet-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemENimustineCat.No.:HY-13703CASNo.:42471-28-3分⼦式:C₉H₁₃ClN₆O₂分⼦量:272.69作⽤靶点:Apoptosis作⽤通路:Apoptosis储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性Nimustine⼀种亚硝脲类烷化剂。Nimustine诱导细胞凋亡，激活DNA损伤以及MAPK信号通路。Nimustine具有抗癌效应，可⽤于癌症的研究。体外研究Nimustine(50μM;72-120h)causescelldeathbyinducingcellapoptosis[1].Nimustine(50μM;24-96h)activatestheDNAdamageresponsepathway[1].Nimustine(50μM;24-120h)activatesMAPKsignalingingliomacells[1].ApoptosisAnalysis[1]CellLine:LN-229celllineConcentration:50μMIncubationTime:72-120hoursResult:Time-dependentlyinducedapoptosis.WesternBlotAnalysis[1]CellLine:LN-229andgliomacelllinesConcentration:50μMIncubationTime:24-120hoursResult:Inducedcleavageofcaspase-8and-9andtheeffectorcaspase-3.Increased1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEphosphorylationofERKkinaseandH2AX.DecreasedphosphorylationofJNK体内研究Nimustine(15fourtimesaweekand30mg/kgtwicewithanintervalof2weeks;i.v.)effectivelyinhibitstumorgrowthandthehigherdoseismoreeffective[2].AnimalModel:FemaleC3H/HeNmicewithsolidFM3Atumors[2]Dosage:15and30mg/kgAdministration:Intravenousinjection;15(4timesaweek)and30mg/kg(twicewithanintervalof2weeks)Result:Theintermittentlarge-doseinjectionsresultedinbetterinhibitionoftumorgrowththandidthefractionatedsmall-doseinjections.REFERENCES[1].TomicicMT,etal.ApoptosisinducedbytemozolomideandnimustineinglioblastomacellsissupportedbyJNK/c-Jun-mediatedinductionoftheBH3-onlyproteinBIM.Oncotarget.2015Oct20;6(32):33755-68.[2].ShimizuF,etal.EffectsofcombinedtreatmentwithnimustinehydrochlorideandradiationonsolidFM3Atumorinmice.JpnJCancerRes.1987Jul;78(7):756-62.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400