进化树构建方法

打开mega5，选择Align——edit/builtalignment--选择DNA/protein出现B521打开mega5，选择Align——edit/builtalignment--选择DNA/protein出现B521:AligiEBentExplores--createanewalignment—OK对话回又DataEdi■Search虹Wei.S*?^ire^cerIli_splayKelpDNASequencesTrarislatedProtein'SequencesLSite#p言C57withrw/oGaps|Open选择已经保存好的用clustalx经过比对保存的以.aln格式的文件我最近的文档桌面我的电痼网上劄居查找范围（X；:|nsoriacQi：〕我最近的文档桌面我的电痼网上劄居e”血瓯序列占TL氨基酸序列贴囹白迎老师取合e新建立件夹叶者师甜会弩V?l.alm我的艾档文件名@）：VTl.alm三|打开职.|文件类型（A：fsujipcirtedlsequencefilTs▼|耳肖打开之后，出现下面的页面fCXCpc・E‘MrLEGG.・G♦,Gc*~~ITTTIT^T.'.-!*-msssmssaswssBuflTTTTTTTTTTTTTTTT*AAAAAAAAAAAAAAA-说*黄.匚TTkTTTTT-r盖遂钠易一直食煎皙■密富擎曷会-SrfCXCpc・E‘MrLEGG.・G♦,Gc*~~ITTTIT^T.'.-!*-msssmssaswssBuflTTTTTTTTTTTTTTTT*AAAAAAAAAAAAAAA-说*黄.匚TTkTTTTT-r盖遂钠易一直食煎皙■密富擎曷会-Sr厥TTT-TTTTTT1■TT斜曩-T-CrTTT赛郭.•工：T;T5.HTTTTTT-^f■-!■:.-■■-匚-•G1--・GG.R,♦.•C..X-一...n..-...©:.•+“

AkA-kk-k?kkk—A-k'A-kk*BusrasuMERMSisB-BWNSEBSA古一s区毒戡一•&.SL■豪■葫岛寸，患冒■♦=■■,塔一♦，*•?■.♦..¥!:.!■.!■TT-'T-IWT'/T.3•工*AA.JmAQ.，•G-A.G.•GM•£••E4=i■部跳.RUSKSm#c:TTTTCTTTTCTTTTT*n3口自匚。自与fJLbEev「式11左521b以AEDEM1：3E之邑FDD952占4一成皿①霍口孙E_F4ikFa7E112：6一FEFJ4L799巳C_LFJL514兆艮CEjg35343乳C3色丫宜5据旧顷C4FJ4E9L5311-C5FJ1-515OODataElitSearch.UigniTrentffebSejjiierLCferH^splayHeljEShm1□若IH瞥*®■1(c龟若X41l>DNASequence^'Trans'lat&dProteinSequences:Sdecies/AtibrvH*/.1*1*土HF*1*rT*r-±-!■*■!■*■dt1■*,!■*■■*■7tI士*1*■*■*■*■|士1*!■*■pmTTTTTTTTTTTT-snK-TT.-委国禹留国窜■■胃兽剪一强重¥曙赧nummaann—AA襄丘L.此la.目A.TTTTTTTTTTTT-snK-TT.-委国禹留国窜■■胃兽剪一强重¥曙赧nummaann—AA襄丘L.此la.目A.菱Hal.A.•枣应屈凹fe-2cccccccccccr-・僵J_・蓝UN之521以AB□.海¥E2：也FDCI9匚芝64一壬后眺辨&口94E_王国好3在1翌6一E5F】m88巳CJLFJLm98研CEAFLMSmU?■匚3每XL&S35SJLU.SFJ4E91.5911_CEFILEISijliTTTtw^h^hwiTTcTTccTr-ccccccc-^^ccccrrrrLrrrLrLrrLrrnLrLrrLTTTTTTTITT-TTTTTTa.aaaaa.a'a-&aaaaaaa'n期■..■an警.•麒...&•£TTTTTTTTTTTTTTTT————————————————•羲警遐一矗尊审崔铸■兽置器程陶房保TCCTTCTCCTCTTTTT一曷爵箱霎-夔费福原技留皙墨盟曾侦谢一...学愚一卷■请.■踱别■整rtisislncisG-C.--Cc:c..-E-G

■T-'TITTIT^J-NCCCCCCC

...s^一琼策度通..携0后◎技..拉■'■■.■之后点击此图内的Alignment选择AlignbyclustalW即可。U-seNe&afciveMaliiKDsl-apDiwiqenlCuioff|箸|KreU-seNe&afciveMaliiKDsl-apDiwiqenlCuioff|箸|KreFl况GrpTie=fParwieAl中hk「土GapCig枷内r凯G^pEnijed^ct'iPeriakpMirfljphAlgrrwril.?Hab||"✓OKIXCarKd默认设置即可，点击OK就进行比对了，此后会出现一个过渡对话框，显示的是两两比对和多序列比对的过程之后回到初始页面，就是这个页面之后点File---点开，把刚才保留的文件点开然后出现下面的页面多了几个内容，点击TA的那个框框。之后出现这样的框框图片然后在主程序中选择phylogeny---construct/testneighbor-joiningtree,然后出现下面的页面黄色框框处的的参数是可以改变的，该图为我已经改变好的，把Bootstrap的值改为1000Methods根据文献上的参考改为了Kimura2-parametermodel.之后点击compute,就出现了，而且还带有必需的支持率

j—C4FJ4G91599?1一FJ95I5b6-X：rX恶&匪萍叮口寿隔!催:「财保留对比时）^区FileImageSubtreeViewCmfriputeCaptionHelp拿电飘0医崔盅走部OriginaltreeBoots,frapidonsen^iistree6702100j—C4FJ4G91599?1一FJ95I5b6-X：rX恶&匪萍叮口寿隔!催:「财保留对比时）^区FileImageSubtreeViewCmfriputeCaptionHelp拿