4-Hydroxywogonin-8-Methoxyapigenin-DataSheet-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemE4′-HydroxywogoninCat.No.:HY-N1904CASNo.:57096-02-3Synonyms:8-Methoxyapigenin分⼦式:C₁₆H₁₂O₆分⼦量:300.26作⽤靶点:IKK;NF-κB;p38MAPK;PI3K;Akt;ReactiveOxygenSpecies;InterleukinRelated;TNFReceptor;Apoptosis;Caspase;Bcl-2Family作⽤通路:NF-κB;MAPK/ERKPathway;PI3K/Akt/mTOR;Immunology/Inflammation;MetabolicEnzyme/Protease;Apoptosis储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性4′-Hydroxywogonin(8-Methoxyapigenin)⼀种酮类化合物，可以从Scutellariabarbata和Verbenalittoralis等多种植物中分离得到。4′-Hydroxywogonin通过TAK1/IKK/NF-κB、MAPKs和PI3/AKT信号通路具有抗炎活性。4′-Hydroxywogonin通过⼲扰PI3K/AKT信号抑制⾎管⽣成。4′-Hydroxywogonin抑制细胞增殖和诱导细胞凋亡(apoptosis)。体外研究4′-Hydroxywogonin(8-Methoxyapigenin;0.5-15μM;0-24h)haslowcytotoxicityandinhibitsNOandPGE2productioninLPS-stimulatedRAW264.7macrophagesbysuppressionofiNOSandCOX-2expression[1].4′-Hydroxywogonin(0.5-15μM;1and12h)suppressesLPS-inducedexpressionofpro-inflammatorycytokinesinRAW264.7macrophagesandsuppressesLPS-inducedactivationofNF-κB[1].4′-Hydroxywogonin(0.5-15μM;1h)suppressesLPS-induceddegradationofIκB-αandactivationofIKKandTAKandsuppressesthephosphorylationofMAPKandAKTininRAW264.7macrophages[1].4′-Hydroxywogonin(0.5-15μM;24h)inhibitsROSproductioninLPS-stimulatedRAW264.7macrophages[1].4′-Hydroxywogonin(0-10μg/mL;24h)reducestheviabilityofSW620ꢀcellsinaconcentration-andtime-dependentmanneranddecreasesthemRNAandproteinexpressionofvascularendothelialgrowthfactor-A(VEGF-A),thepredominantpro-angiogeniccytokineintumorangiogenesis[2].4′-Hydroxywogonin(24h;SUP-B15andJurkatcells)inducesapoptosisanddecreasestheexpressionofC-MYC,BCL-2andcleavedcaspase3[3].1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemECellViabilityAssay[1]CellLine:RAW264.7macrophagesConcentration:0.5,5and15μMIncubationTime:24hoursResult:HadlowcytotoxicityinRAW264.7macrophages.WesternBlotAnalysis[1]CellLine:RAW264.7macrophagesConcentration:0.5,5and15μMIncubationTime:1hoursResult:AttenuatedtheincreaseofiNOSandCOX-2mRNAexpressioninducedbyLPSinRAW264.7cells.WesternBlotAnalysis[1]CellLine:RAW264.7macrophagesConcentration:0.5,5and15μMIncubationTime:1and12hoursResult:ReducedTNF-α,IL-6andIL-1βmRNAexpressioninadose-dependentmanner.InhibitedLPS-inducedp65phosphorylationandnucleartranslocation.WesternBlotAnalysis[1]CellLine:RAW264.7macrophagesConcentration:0.5,5and15μMIncubationTime:1hoursResult:AttenuatedLPSinducedIκB-αdegradation.AttenuatedthephosphorylationofERK1/2andp38inadose-dependentmanner.ReducedtheintensityoftheTAK1/TAB1band.WesternBlotAnalysis[2]CellLine:SW620cellsConcentration:0.1,1,and10μg/mL2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEIncubationTime:24hoursResult:DownregulatedVEGF-Aexpressionincolorectalcancercellsandsuppressedangiogenesis.体内研究4′-Hydroxywogonin(10and20mg/kg;i.p.;maleC57BL/6mice)alleviatesLPS-inducedacutelunginjury(ALI)inamousemodel[1].AnimalModel:MaleC57BL/6mice(6-8weeksold;20g)withacutelunginjurymodel[1]Dosage:10and20mg/kgAdministration:Intraperitonealinjection,12and1hbeforeLPStreatmentResult:HadpotentialprotectiveeffectsagainstinflammationinLPSinducedALImice.Attenuatedthedegreeofleukocyteinfiltration.REFERENCES[1].FanC,et,al.4'-Hydroxywogoninsuppresseslipopolysaccharide-inducedinflammatoryresponsesinRAW264.7macrophagesandacutelunginjurymice.PLoSOne.2017Aug8;12(8):e0181191.McePdfHeightCaution:P