ABI3500操作手册基因测序

ABI3500操作手册ABI3500操作手册x/xx/xABI3500操作手册ABI3500操作手册x/xx/x3500/3500XL简易操作手册1.空间定位和光谱校正1.1设置DyeSet进入library界面，选择DyeSets菜单，点击Create,在DyeSetName中输入“AGCU_E5”作为我公司DyeSet名称，从Chemistry下拉菜单中选择“MatrixStandard”，从DyeSetTemplate下拉菜单中选择“E5Template”，设置完成后点击Save保存设置。35QDDnfcflCon«ctFanSnfbji-gir-aDMlibo^rdEdft■嚙|嚙|Lifirary 已占SBCruft 1Duplicatejfj营［mpurtijA£-£ign.si：iir?_Vvl颗QuditrlhFo1 VrsixF-5ignrti.-reHi-ioiyMCUAn*ILSrMCUAn*ILSr貿Firr^sraaSmmEoeRr^.ftsGniiEiLi丸土匸*hxtrurrartftt±occt>，辻白m白rKJ間iJ。whFafrctcmBlSizer?|r>3FMeBb;■aCFndwdB&«^jH-icriqAralyxah-trlnoolEM如叫DPt-otaeotaFiAQn-^ntAnAlN化PfcLocdiupu-■-.-丄1.2空间定位进入3500DataCollectionSoftware主界面，选择Maintenance菜单，在Maintenance界面中点击Calibrate下拉菜单中的Spatial按钮，选择合适的空间定位参数即可开始空间定位。3500系列遗传分析仪空间定位与31003130系列类似。

1-^3111Edx'■LihfjrpNahiutiaru'*1-^3111Edx'■LihfjrpNahiutiaru'*「wkFJaru^df兼蜘NfffWfwe J¥kwSprtialCdiiiilMinH^piirt土>hi忒▼| |•r；&，，.、》=折宀、龍宀七TrbktkKfrrkiQ9Lm4kr4Pib^w4trdHlSiinitardK顷5igMUSnrt-rir；&，，.、》=折宀、龍宀七TrbktkKfrrkiQ9Lm4kr4Pib^w4trdHlSiinitardK顷5igMUSnrt-riU・!wJdk,0|rt!W!lFdl省中却-Rci^WT!!■OC<h«kihuriMMMWV/MMK^UWMUvEwMrw**r・tu W«iqJ%wfci.f •烦\Hr>tcNiwl寻pmIhen^>ruF!cnh4?wJ'nngaipaiiri■:dhtriMifiilmi.1.2光谱校正a、光谱校正前先更换水和阴阳极Buffer,按10ul甲酰胺：0.5ul5DyeMatrixStandard的比例配制甲酰胺和光谱校正标准品混合物，轻轻震荡混匀后短暂离心。准备一块新的96孔反应板，将配制好的混合物均匀的分装在前三列各孔中。3500/3500XL进行光谱校正时仪器默认第一个Run，即前三列中加入了光谱校正混合物。95°C加热3min，立即冰浴3min,变性完成后装载96孔板并置于仪器托盘中。b、点击Calibrate下拉菜单中的SpectraI按钮，按照图示选择各种参数点击StartRun即可进行光谱校正。注意在DyeSet中应选则在1.1中设置好的与AGCU试剂盒相匹配的DyeSte。如果光谱校正未达到仪器默认质量要求，仪器会自动重复跑样三次，并自动选择各次跑样中质量最好的通道作为此次光谱校正的最后结果。光谱校正时应观察实时原始数据，以便及时做出相应调整，确保最终结果。C、3500系列遗传分析仪与3100、3130系列遗传分析仪最大的区别之一就是光谱校正。它不需要编写protocol、runmodule等软件栏目及其相应参数设置，直接按照仪器默认的参数进行即可，因此操作更为简单、快捷。

督iUUl卅nFjL<jr«yh%■诉rwnw:TariiMinqc■"PiHcnm>，督iUUl卅nFjL<jr«yh%■诉rwnw:TariiMinqc■"PiHcnm>，L/gOvI2.软件参数设置2.1创建InstrumentProtocol进入library界面，选择InstrumentProtocols菜单，点击Createo按下图所示选择或设置各个参数，设置完成后点击Save保存。注意将ProtocolName命名为“AGCU_E5_STR”，进样电压设置为3kv，进样时间可设置为6〜10sec，DyeSet选择1.1步骤中已设置好的“AGCU_E5”，其他参数设置请参照下图。■WHfmn舟CEeoUmi*”rr*占展Dmlnb-aar-clE-skl:""Libraryhliintanii-K-iT«h*Mariana淼FTssaurcw3iCreateEdfr四口叩hcrterflDie^ete[tt]mf)iort(9Ewrt|||E-$iBn¥bunflVwwAiHirtHistoryEl■丨9；Mch:□Irijn-14ACCUJS.STRPrslacDlPro■WHfmn舟CEeoUmi*”rr*占展Dmlnb-aar-clE-skl:""Libraryhliintanii-K-iT«h*Mariana淼FTssaurcw3iCreateEdfr四口叩hcrterflDie^ete[tt]mf)iort(9Ewrt|||E-$iBn¥bunflVwwAiHirtHistoryEl■丨9；Mch:□Irijn-14ACCUJS.STRPrslacDlPro!FunM<i<|iikHlD]-5_P<iP-l-le-i^iii2ioii.ElLochtdOpenTtii-iptrftUiieCPC).如RunVotenqe敝"luhD.QPieEyiiVoka<h!华剧>lu| 15Votuge^.¥ofe).?.!■flunniiwR«c)moFVeRuiLTnfiti11。biJveod^Hnine^«,lDrwnDtla^(i«c、■AdvMmlU'lblMvnFollowr'igfdlu七5Tvtiwcitcoinn'KiKl^lUed>iiiq<Nl.VohwTofeMiKe n1「由触把ihrtTime侦妨 ISOVol^jge*offtejfij-4nilVoiraflt5itp ««》女tmdReadtiurTp^i<ImsINnrnifl^E-ib-^iF-urtwIhrtah^kiMii'2.2创建SizeStandard进入library界面，选择SizeStandards菜单，点击Create。在SizeStandard中输入“AGCU_SIZ500”，DyeColor下拉菜单中选择“Orange”，在左侧对话框中依次输入75、100、139、150、160、200、300、340、350、400、450、490、500，每次输入结束后点击中间“AddSize”菜单即可将所输数字加入右侧对话框中。待全部输入结束后点击Save保存。ABI3500操作手册ABI3500操作手册x/xx/xABI3500操作手册ABI3500操作手册x/xx/x OflMlboarri Lihr-iri-MjinttnirsrTcwih-Mjnny?*Cre-arre呈E・m』加卩>占時 E-ti 1hV<ft(SExport#|E-SfertrhJ-t,WwAwdfcHtstDry.UlEwE-Slgnvtu OflMlboarri Lihr-iri-MjinttnirsrTcwih-Mjnny?*Cre-arre呈E・m』加卩>占時 E-ti 1hV<ft(SExport#|E-SfertrhJ-t,WwAwdfcHtstDry.UlEwE-Slgnvtu忙HlftoryS?hpqS»roSlarwlard WK] □L«scl<e'1 Gn全imtLird Q'fbcnpfcton: Cirangt七 £-'Itta'jyxi'nhSr.hBi'abin'ctH-r-aparchr^dr.-3n<c<T：mn.apfcr.a*-nrhizMbt?citrAt^i<^Lei't'--'由讶iw>ZsacNmefatharwriiin^airwEhmrsdcTnnr/ir>fio*i□wetmwStKKamdEd C«>■LL5I 匸li*IEiA♦宁・nH-rdd«和rh>3ri= 0」 如Id血fl•JOS.*JB.I-囱.小J4d.l2W>映潯JM.I4■强,♦2.3创建QCProtocol进入library界面，选择QCProtocols菜单，点击Create。在ProtocolName中输入“AGCU_QC”，SizeStandard下拉菜单中选择在2.2 中已创建的“AGCU_SIZ500”，将UseBaselining改为38。其他参数均按下图 所示进行设置，设置完成后点击Save保存。ABI3500操作手册ABI3500操作手册ABI3500操作手册ABI3500操作手册x/xx/xS35^0D-rta-C^llecuonDmhEcwaE^lri，LibrniyM-urtfranrelo^h♦ ■> ■月ngunMi _|9Crem.JEW色t0DeldflijfB-vipon>ort|hE"Sgn3tuRSolupaOCProiocolPr4tcr=-alHih-hDvacnphainSsvJiidnrd:AdCU.ilZiQO材EiSllnvLL。'"-jJ-iUlYSiiSSTDIifliQ<IPnnial-Ah^Iy^IsPctii：2^nnILEed■StolTgR*ltx ParTHli■*■I5te>ng虫mt#时MSolupaOCProiocolPr4tcr=-alHih-hDvacnphainSsvJiidnrd:AdCU.ilZiQO材EiSllnvLL。'"-jJ-iUlYSiiSSTDIifliQ<IPnnial-Ah^Iy^IsPctii：2^nnILEed■StolTgR*ltx ParTHli■*■I5te>ng虫mt#时M如ngW卩Ssw5SimCallmyM«h*xi|Loc-al-SoirImin™FriiH*：■C^mnionfixings\/lBki*回<5r*enJFed■『511751T5175PeakAinpliiiideThresholdUs«LlivEm，lh*dng{BjLdfinvWiiiflawMn-iiniuniF'vikHarfWiditiPmk ShePdycior'ihjJCHgr-i4■SlnpeTlhiHholdPea〉EndSlapaThnahaklP«nkShirt[75■Clm2.4创建Assay进入library界面，选择Assays菜单，点击Create。在AssayName中输入“AGCU_STR”，ApplicationType下拉菜单中选择“HID”，Instrumentprotocol下拉菜单中选择2.1中创建的“AGCU_E5_STR”，QCProtocol下拉菜单中选择2.3中创建的“AGCU_QC”。待所有选项选择完成后点击Save保存。若第一步是从创建Assay开始，也可在该界面下点击“CreateNew”菜单，按照上文所述要求逐一创建新的InstrumentProtocol、QCProtocol并将其保存至Library中，其效果与前面创建现在调用一样。7WS5i<iLJ?rtaCoihtctionSflftwnreCmkhho-irtlEditLitjrjirySPCr«*6eEditE3Ducili.jte■既应皂i*Tmgrt：tW& :#|E-釦如加im VTewAuditHirtn.iyViewFlBtEAs-mp-5FMOjnvflHicnisResultsOro#Fittd-l.Ezrch.rrSfll-LjfiC-rf少iDbZi亡I：松SizeSiN>diar£4&□nrec^ilngPnrtjwnls口rEgpl乎<j<：prdi>3ok^c-qufrncni^iAndv^±Pf-oidcqeMiDfaSetlDPrctiiccisFrflfluerriArwig。ProtoonhhcirwfysrsFrrtarol'j2.5创建FileNameConvention进入library界面，选择FileNameConventions菜单，点击Create。在Name中输入“AGCU_Samplename”，其他参数按下图进行设置，也可根据个人喜好进行更改。注意在SelectFileLocation中选择CustomFileLocation,可以先在硬盘新建文件夹，此处点击Browse进行选择。待所有设置完成后点击Save保存。Di-ihh^-aidEdit甘Lfariry1■-侦rrlmanw "Fpoii■Meww■MG+rt*■'£dri [Xqpftc-vl-F函[FW弓 ImportJBfx|inrt员■i- -\>invAurlftHnA^iy匸E点》■F#¥rM・ni/A^'jtU_^iiinpl^nwin'*W：・姑.伽岸E・l・(xmFilkiN-cwn-HOcrnvhirrfiiMirjairr*厶nhm卩IdiLbrn*^vTalablri-Am*亠wAj-nph<«inMiiipfPi-rtvn-?>N»jnrAiHaihuLip^irF^Mum^xiClheayiliiitKrnT«d2i.i.KW*Yllk・FWe】伽师n>nHai-nai^Liiiilild-Film*Fnlova*L^pr.|*>-•?■'o.ntcm■■ •姓”b・rtnCHfi^ii-'iT+<liCu-：E4i'nT+«t1iS*i*efcFihL^crtwn，LiffinhFt*ILacilinnE>、師日MdBwhyiMEiHRU'心浸■■■»CutMomFlhL#<sbci|i □•••.淳Bnmxx/X2.6创建ResultGroup进入library界面，选择ResultGroups菜单，点击Create。在Name中输入“AGCU_Resultgroup”,其他参数按下图进行设置,也可根据个人喜好进行更改。注意在SelectFileLocation应与2.5中选择CustomFileLocatio位置相同。待所有设置完成后点击Save保存。'Ham,3顷日mbGirtlipim|h中>PrtfBScirtk~ntTuiiiiintKunTimaid«1 -mi(mwbmi'ivi!Piww阪mKfUltih&TDL|1l-UmdEiricr■'curfwn'Ham,3顷日mbGirtlipim|h中>PrtfBScirtk~ntTuiiiiintKunTimaid«1 -mi(mwbmi'ivi!Piww阪mKfUltih&TDL|1l-UmdEiricr■'curfwn由ut■?■n'W'crttirPkI1".vi 触卩Inndlh-di-SdttCRi»!>j4KIK"iF<kir-OpIK"*ilwjrsir|¥^Mni^FfihFi*!m■*1寸1—R-iri^-rti-tinhiHaiknm4h+vi*lwihjaetaanI■: witii 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>,ihmAiirfrfcHnbary'2.7创建PlateTemplatea、进入library界面，选择AssignPlateContents菜单，在NewPlate下拉菜单中选择CreatePlatefromTemplate，在该界面中选择软件自带的ABI样品表模板“5dye_36_POP4”双击打开。b、将ABI样品表模板“5dye_36_POP4”中Assays、FileNameConventions和ResultsGroups栏目下的各可选项逐一删去。c、在Assays、FileNameConventions和ResultsGroups各栏目下点击Addfromlibrary，分别向各栏目中加入AGCU_STR、AGCU_Samplename和AGCU_Resultgroup。d、各栏目选项加入完成后点击SavePlate菜单，选择Saveas后输入“AGCU_Platetemplate”，点击ok保存即可。ABI3500操作手册ABI3500操作手册x/xx/xABI3500操作手册ABI3500操作手册x/xx/x,JS3M0Dn?Gc赂ECflWfeiirt，丑"：DiMiliwiEiil:*Libra瑁MirritninctTwh*MinigrvPMfirflKnHdp卩Log<IUt|_Am御g蝕电^PClWUP\v»-i_PQpfiiPkK：*|_SRnt卩瞄！■昌晚f.昂E*硕丄^Fifrl网ilw*WVtew円m尊廿由网沢白Piirr晦訂卩岫也5密口bkWwCrftn?Hr|?Prflyrh^Crftn?Hr|?Prflyrh^R>inrciHjSieniLMdPMfLIM細AwhWi'RuiJ.Ra-iWi*HailJe■rtsw:三加車女梱Fk^ufteiWZFimmlhD快h土叵］E叵］E心，"M.（jFN.ffa5!54y_KHM_D<ICiMei_HGPmJGPNVFiiuwR^Aiaifi_F・htaawttwfcmn与□ /f£J®JWHI.5+F-rtkflime_<J』日□□□□□□»».也M0IjsIQ口口□

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