DL-Sulforaphane-N-acetyl-L-cysteine-SFN-NAC-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors • ScreeningLibraries • Proteinswww.MedChemEDL-SulforaphaneN-acetyl-L-cysteineCat.No.:HY-132242CASNo.:334829-66-2Synonyms:SFN-NAC分子式:C₁₁H₂₀N₂O₄S₃分子量:340.48作用靶点:HDAC;Apoptosis;DrugMetabolite作用通路:CellCycle/DNADamage;Epigenetics;Apoptosis;MetabolicEnzyme/Protease储存方式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY生物活性DL-SulforaphaneN-acetyl-L-cysteine(SFN-NAC)是一种具有口服活性的HDAC抑制剂，也是sulforaphane(HY-13755)代谢物且具有更长的半衰期和更好的血脑屏障通透性。DL-SulforaphaneN-acetyl-L-cysteine通过ERK途径激活自噬介导的α-微管蛋白表达下调，可用于癌症的研究。体外研究SFN-NAC(24h)decreasescellviability,withIC50valuesof60.08μMforHA,35.20μMforU87MG,39.11μMforU373MG,and36.20μMfortheU87/TRcells[1].CellCycleAnalysis[1]CellLine:U87MGandU373MGcellsConcentration:0,30μMIncubationTime:24hResult:Inducedcell-cyclearrestintheG2/Mphaseandtriggeredapoptosisatthesametime.WesternBlotAnalysis[1]CellLine:U87MGandU373MGcellsConcentration:0,10,20,30,40,50μM1/2 MasterofBioactiveMolecules—您身边的抑制剂大师www.MedChemEIncubationTime:24hResult:ActivatedERK1/2(Thr202/Tyr204),downregulatedα-tubulin,andinducedautophagyinadose-dependentmanner.CellViabilityAssay[1]CellLine:HA,U87MG,U373MGandU87/TRcellsConcentration:0,10,20,30,40,50,60,70,80,90μMforHAandU87MGcellsor0,10,20,30,40,50,60μMforU373MGandU87/TRcellsIncubationTime:24hResult:Decreasedthecellviabilityofthesecelllinesinadose-dependentmanner.体内研究SFN-NAC(10μmol;6h;Oralgavage;singledose)significantlyinhibitsHDACactivityinmousecolonmucosa[2].AnimalModel:mice[2]Dosage:10μmolAdministration:Oralgavage;6h;singledoseResult:SignificantlyinhibitedHDACactivityinmousecolonmucosa.REFERENCESLiuHJ,etal.Sulforaphane-N-Acetyl-CysteineInducesAutophagyThroughActivationofERK1/2inU87MGandU373MGCells.CellPhysiolBiochem.2018;51(2):528-542.DashwoodRH,etal.Dietaryhistonedeacetylaseinhibitors:fromcellstomicetoman[C]//Seminarsincancerbiology.AcademicPress,2007,17(5):363-369.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly