Iberverin-3-Methylthiopropyl-isothiocyanate-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors • ScreeningLibraries • Proteinswww.MedChemEIberverinCat.No.:HY-121204CASNo.:505-79-3Synonyms:3-Methylthiopropylisothiocyanate分子式:C₅H₉NS₂分子量:147.26作用靶点:Apoptosis;ReactiveOxygenSpecies作用通路:Apoptosis;Immunology/Inflammation;MetabolicEnzyme/Protease;NF-κB储存方式:Pureform-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:100mg/mL(679.07mM;Needultrasonic)SolventMass1mg5mg10mgConcentration制备储备液1mM6.7907mL33.9536mL67.9071mL5mM1.3581mL6.7907mL13.5814mL10mM0.6791mL3.3954mL6.7907mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存方式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。BIOLOGICALACTIVITY生物活性 Iberverin(3-Methylthiopropylisothiocyanate)是萝卜硫素同系物。Iberverin具有抗癌活性。Iberverin抑制细胞增殖和迁移。Iberverin诱导线粒体相关细胞凋亡(Apoptosis)和细胞内活性氧(reactiveoxygenspecies)[1]。1/2 MasterofBioactiveMolecules—您身边的抑制剂大师www.MedChemE体外研究Iberverin(0-200μM,48h)inhibitstheviabilityandproliferationofHCCcells,withIC50slessthan25μMforHuh7,Huh7.5.1,andSNU739[1].Iberverin(10μM,24-72h)inhibitsmigrationandinvasioninHuh7,Huh7.5.1andSNU739cells[1].Iberverin(40μM,12h)inducesmitochondrial-relatedapoptosis,inducesDNAdamageandcausesG2/McellcyclearrestinHuh7,Huh7.5.1andSNU739cells[1].WesternBlotAnalysis[1]CellLine:Huh7,Huh7.5.1andSNU739cellsConcentration:40μMIncubationTime:12hResult:EnhancedthelevelofapoptoticproteinBaxbutrepressedtheexpressionofBcl-2.体内研究Iberverin(20mg/kg,i.p.,every3daysforfivecycles)inhibitsthegrowthofHCCxenografttumorinmice[1].AnimalModel:Huh7.5.1cellsweresubcutaneouslyinjectedintoimmunodeficientBALB/cnudemice[1]Dosage:20mg/kgAdministration:i.p.,every3daysforfivecyclesResult:Reductionthetumorsizeby73.4%andweightby55.3%ofHuh7.5.1xenografttumors,withnosystematictoxicity.DecreasedKi-67andPCNAlevelintumor.REFERENCES1.ZhangY,etal.IberverinexhibitsantineoplasticactivitiesagainsthumanhepatocellularcarcinomaviaDNAdamage-mediatedcellcyclearrestandmitochondrial-relatedapoptosis.FrontPharmacol.2023Dec13;14:1326346.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuse