DiI-solution-DiIC18-3-solution-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors • ScreeningLibraries • Proteinswww.MedChemEDiI(solution)Cat.No.:HY-D0083YCASNo.:41085-99-8Synonyms:DiIC18(3)(solution)分子式:C₅₉H₉₇ClN₂O₄分子量:933.87作用靶点:FluorescentDye作用通路:Others储存方式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY生物活性DiI(solution)(DiIC18(3)(solution))是一种长链碳菁染料。碳菁染料广泛用作亲脂性示踪剂，用于标记细胞、细胞器、脂质体、病毒和脂蛋白。体外研究CarbocyaninedyesarewidelyusedasDitolabelcells,organelles,liposomes,virusesandlipoproteins.Long-chaincarbocyanineswhichincludeDiO(DiOC18(3)),DiI(DiIC18(3)),DiD(DiIC18(5))andDiR,anddialkylaminostyryldyeDiA(4-Di-16-ASP)areusedforlabelingmembranesandotherhydrophobicstructures.DiIC16(3)hasshorteralkylsubstituents(C16)thanDiI(C18).Theyhaveextremelyhighextinctioncoefficients,environmentaldependentfluorescenceandshortexcited-statelifetimesinlipidenvironments.Theyareoilsatroomtemperatureandweaklyfluorescentinwaterbuthighlyfluorescentandquitephotostablewhenincorporatedintomembranesorboundtolipophilicbiomolecules.Theseopticalcharacteristicsmakethemidealforstainingthecytoplasmicmembranesofcells.Onceappliedtocells,thesedyesdiffuselaterallywithintheplasmamembrane,resultinginstainingoftheentirecell[1].DiO,DiI,DiDandDiRexhibitdistinctgreen,orange,redandinfraredfluorescence,respectivelythusfacilitatingmulticolorimagingandflowcytometricanalysisoflivecells.DiOandDiIcanbeusedwithstandardFITCandTRITCfiltersrespectively.AmongthemDiIanditsanalogsaremostfrequentlyusedsincetheyusuallyexhibitverylowcelltoxicity.Inaddition,DiIiswidelyusedfordetermininglipoproteinssuchasLDLandHDL.ThelipophilicaminostyryldyeDiAisalsooftenusedforneuronaltracing[2].GeneralProtocol1.PreparingStainSolutionsofDia.PrepareDMF,DMSOorethanolstocksolutions:Thestocksolutionsshouldbepreparedindimethylformamide(DMF),dimethylsulfoxide(DMSO,orethanolDMSOat1-5mM.DMFispreferabletoethanolasa1/2 MasterofBioactiveMolecules—您身边的抑制剂大师www.MedChemEsolventforDi.Thestocksolutionshouldbeusedpromptly.Anyunusedsolutionneedtobealiquotedandrefrozenatleast-20℃.Avoidrepeatedfreeze/thawcycle.Thesolutioncanbestoredfor6months.b.Prepareworkingsolutions:Dilutethestocksolutionsintoasuitablebuffersuchasserum-freeculturemedium,HBSSorPBStomake1to5μMworkingsolutions.Wedonotrecommendstoringtheaqueoussolutionformorethanoneday.Note:Thefinalconcentrationoftheworkingsolutionshouldbeempiricallydeterminedfordifferentcelltypesand/orexperimentalconditions.2.Suspensioncellsa.Centrifugeat1000gat4℃for3-5minutesandthendiscardthesupernatant.WashtwicewithPBS,5minuteseachtime.Thecelldensityis1×106/mL.b.Add1mLofDiworkingsolution,andthenincubateatroomtemperaturefor5-30minutes.c.Centrifugeat400gat4℃for3-4minutesandthendiscardthesupernatant.d.WashtwicewithPBS,5minuteseachtime.e.Resuspendcellswithserum-freecellculturemediumorPBS.Observationbyfluorescencemicroscopyorflowcytometry.3.Adherentcellsa.Cultureadherentcellsonsterilecoverslips.b.Removethecoverslipfromthemediumandaspirateexcessmedium.c.Add100μLofworkingsolution,gentlyshakeittocompletelycoverthecells,andthenincubateatroomtemperaturefor5-30minutes.d.Washtwicewithmedium,5minuteseachtime.Observationbyfluorescencemicroscopyorflowcytometry.体内研究 DiI-labeledmotoneuronshaveremainedviableforupto4weeksincultureanduptooneyearinvivo[1]客户使用本产品发表的科研文献CancerCell.2024Apr8;42(4):682-700.e12.AdvFunctMater.2024May9.ChemEngJ.2024Feb16,149761.JNanobiotechnology.2025Jan22;23(1):34.FoodHydrocoll.2025Dec.Seemorecustomervalidationsonwww.MedChemEREFERENCESGanWB,etal.Multicolor"DiOlistic"labelingofthenervoussystemusinglipophilicdyecombinations.Neuron.2000Aug;27(2):219-25.DPKuffler,etal.Long-termsurvivalandsproutinginculturebymotoneuronsisolatedfromthespinalcordofadultfrogs.JCompNeurol.1990Dec22;302(4):729-38.DPKuffler,etal.Long-termsurvivalandsproutinginculturebymotoneuronsisolatedfromthespinalcordofadultfrogs.JCompNeurol.1990Dec22;302(4):729-38.McePdfHeight2/2 MasterofBioactiveMolecul