CN119954921A 谷子控制分蘖数目和有效穗数基因Sisd1及其应用

(12)发明专利申请(10)申请公布号CN119954921A(21)申请号202510250128.1(71)申请人山西农业大学权利要求书2页说明书18页序列表(电子公布)附图4页(57)摘要21.SiSD1基因或其编码的SiSD1蛋白在获得有效穗数增加、有效分蘖数增加和/或产量3)与SEQIDNO.5所示的氨基酸序列具有c)与SEQIDNO.4所示的核苷酸序列具有至少34谷子控制分蘖数目和有效穗数基因Sisd1及其应用5[0013]3)与SEQIDNO.3所示的氨基酸序列具有至少90%,优选具有至少99%同一性，67[0046]在一个实施方案中，用于构建所述重组载体的质粒可以选自，不限于8附图说明9序列ATGCGGTGCAACTACTACCC(SEQIDNO.8)GGGTAGTAGTTGCACCGCAT(SEQIDNO.11)序列GCGTGGTGGCTCAAATTGTT(SEQIDNO.13)AACCGGAGTGTTTCATCCCC(SEQIDNO.15)ACCAGCTTCTGTGACGAGTG(SEQIDNO.16)CTTCTCGATGGACCTGAGCG(SEQIDNO.1CGTCATGTTCCTGCACAAGC(SEQIDNO.1CTCGAACACCGCCGAGTAG(SEQIDNO.19)CTCCCTCTCTTCCCGACTGG(SEQIGCTGCTGGATCGTAACGGAA(SEQIDGACCAGGTCATGTCCGAGGT(SEQID验获得的阳性植株pSiSD1::SiSD1、野生型植株JG21和实施例3所述的近等基因系植株[0093]实施例6.过表达SiSD1植株单株有效分ChenJ,ZhaoY,WangJ,WuX,WangM,ZhangY,LiuB,WuY,HarbEnhancedsustainablegreenrevolutionyieldvmodulationinrice.Science.2020Feb7;367(6478):eaaz2046.May20;73:523-551.Greenrevolution:amutantgibbApr18;416(6882):701H,WangB,GaoStrigolactoneBiosynthesisGeneContributedtoBealesJ,FishLJ,WorlandAJ,PelicaGaleMD,HarberdNP.'Greenrevolution'genesencoderesponsemodulators.Nature.1999Jul15;400(6741):256-61.RohdeA.N-terminaltruncatedRHT-1proteinsgenreinitiationcausesemi-dwarfingofwheatGreenRevolutionPlant.2021Apr5;14(4):679-687.J,NiZ.AnaturalvariationinRibonucleaseH-likegeneunconfer"GreenRevolution"traitinwheat.MolPlant.2022Y,LiY,ZhangJ,WangcharacterizationofRht8,a"GreenRevolution"replacementgeneinwheat.MolPlant.2022Mar7;15(3):373-376.DELLAtranscriptionalreprogramingunderliessefoxtailmillet[J].TheCropJournal,2019,7(4):458-468.X.CharacterizationandfinemappingofSiDWARF2(D2)infoxtailmillet[CropScience,2016,56(1):95-103..multi-environmentusinganultra-highdensityBiAppliedGenetics,2021,134(2):557-572.inmaize[J].Nature,1997,386(6624):485-488.M,MatsuokaM,UeguchiC.TheOsTB1genenegativelybranchinginrice.PlantJ.2003Feb;33(3):513-20.milletzip-likegene,SiPf40,intransgeniphenotypeaffectingtillering,vasculardistributionSciChinaLifeSci.2010Dec;53(12):1450-8.act:howplantsintegratenitrogenandwatersig71(15):4442-4451.ofFoxtailMillet(SetariaPerspectiveofResponsestoNitrogenandPhosphateLimitations.FrontPlantSiMYB3inFoxtailMillet(Setariaitalica)ConfersTolerancetoLX.Sensitivitytogibberellinofdwarffoxtailmilletvarieties[J].Cropscience,2012,52(3):1068-1075.(03):120-122.[0123]SEQIDNO.1:谷子R尽管上面已经示出和描述了本发明的实施例，可以理解的是，上述实施例是示例22AAdnrt:dnrt:DBS.viridisv2.1|Sevir.3A.thalianaTAIR10|AT1G44090.1CCS.viridisv2.1|Sevir.923AAActin-P