Fluo-4-AM-solution-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEFluo-4AM(solution)Cat.No.:HY-DY1028CASNo.:273221-67-3分⼦式:C₅₁H₅₀F₂N₂O₂₃分⼦量:1096.95作⽤靶点:FluorescentDye作⽤通路:Others储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性Fluo-4AM(solution)⼀种常⽤的检测细胞内Ca2+(钙离⼦)浓度的探针(Ex/Em=485/526nm)[1]。溶剂及浓度：DMSO：1mM体外研究Guidelines(Followingisourrecommendedprotocol.Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds).1.PreparationofworkingsolutionDilutewithbuffer(e.g.HHBScontaining0.04%Poloxamer407(F127)(HY-D1005))(optimizedaccordingtotheexperiment).Thecorrespondingstocksolutioncanbedilutedaccordingtotheactualsituation.NotethatifthesolventisDMSO,thecytotoxicityofDMSOmustbeconsidered,andasolventcontrolshouldbeprepared;ifthesolventispurewater,theworkingsolutionneedstobefilteredandsterilizedbeforeaddingcells.Note:Theworkingsolutionshouldbepreparedandusedimmediately,andprotectedfromlight.2.Cellstaining2.1Countthecellsandtake106cellsfromeachsample(controlandexperiment/s).2.2Collectthecells(5min,3000×g,4°C)andwashonceinPBS.2.3Resuspendthecellsin0.5mLPBSandadd0.5μLofFluo-4-AM(1mMstock)toafinalconcentrationof1μM.Incubateat37°Cfor1h.2.4Washthecellsthreetimes(2min,3000×g)withPBSandfinallyresuspendin1mLPBS.2.5Evaluatethestainingbyflowcytometry(Ex/Em=485/526nm).REFERENCES1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE[1].GeeKR,etal.Chemicalandphysiologicalcharacterizationoffluo-4Ca(2+)-indicatordyes.CellCalcium.2000Feb;27(2):97-106.[2].Fluo-4.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-379