MitoTracker-Green-FM-solution-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEMitoTrackerGreenFM(solution)Cat.No.:HY-DY1032CASNo.:201860-17-5分⼦式:C₃₄H₂₈Cl₅N₃O分⼦量:671.87作⽤靶点:FluorescentDye作⽤通路:Others储存⽅式:-20°C,protectfromlight.Avoidrepeatedfreezingandthawing*Insolvent:-80°C,6months;-20°C,1month(protectfrom

light.Avoidrepeatedfreezingandthawing)BIOLOGICALACTIVITY⽣物活性Mito-TrackerGreen(solution)⼀种绿⾊荧光染料，可以选择性地积聚在线粒体质中。MitoTrackerGreenFM通过与半胱氨酸残的游离硫醇反应共价结合线粒体蛋⽩，从⽽可以不依赖膜电位即对线粒体膜电位进⾏染⾊。激发/发射波长490/523nm。溶剂及浓度：DMSO：1mM体外研究GeneralProtocol1.PreparationofMitoTrackerGreenFMworkingsolution1.1StocksolutionItisrecommendedtostorethestocksolutionat-20°Cand-80°Cawayfromlightandavoidrepetitivefreeze-thawcycles.1.2PreparationofMitoTrackerGreenFMworkingsolutionDilutethestocksolutioninserum-freecellculturemediumorPBS.Thecorrespondingstocksolutioncanbedilutedaccordingtotheactualsituation.NotethatifthesolventisDMSO,thecytotoxicityofDMSOmustbeconsidered,andasolventcontrolshouldbeprepared;ifthesolventispurewater,theworkingsolutionneedstobefilteredandsterilizedbeforeaddingcells.Note:PleaseadjusttheconcentrationofMitoTrackerGreenFMworkingsolutionaccordingtotheactualsituation.2.Cellstaining2.1Suspensioncells(6-wellplate)a.Centrifugeat1000gat4°Cfor3-5minutesandthendiscardthesupernatant.WashtwicewithPBS,5minuteseachtime.Thecelldensityis1×106/mL.1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEb.Add1mLofworkingsolution,andthenincubateatroomtemperaturefor15-45minutes.c.Centrifugeat400gat4°Cfor3-4minutesandthendiscardthesupernatant.d.WashtwicewithPBS,5minuteseachtime.e.Resuspendcellswithserum-freecellculturemediumorPBS.Observationbyfluorescencemicroscopyorflowcytometry.2.2Adherentcellsa.Cultureadherentcellsonsterilecoverslips.b.Removethecoverslipfromthemediumandaspirateexcessmedium.c.Add100μLofworkingsolution,gentlyshakeittocompletelycoverthecells,andthenincubateatroomtemperaturefor5-30minutes.d.Washtwicewithmedium,5minuteseachtime.Observationbyfluorescencemicroscopyorflowcytometry.StorageStoreinseparatecontainerstoavoidrepeatedfreeze-thawcycles.-20°C,1year.ProtectfromlightPrecautions1.PleaseadjusttheconcentrationofMitoTrackerGreenFMworkingsolutionaccordingtotheactualsituation.2.ThisproductisforR&Duseonly,notfordrug,household,orotheruses.3.Foryoursafetyandhealth,pleasewearalabcoatanddisposableglovestooperate.REFERENCES[1].GautamN,et,al.AhighcontentimagingflowcytometryapproachtostudymitochondriainTcells:MitoTrackerGreenFMdyeconcentrationoptimization.Methods.2018Feb1;134-135:11-19.[2].GautamN,et,al.AhighcontentimagingflowcytometryapproachtostudymitochondriainTcells:MitoTrackerGreenFMdyeconcentrationoptimization.Methods.2018Feb1;134-135:11-19.ꢀMcePdfHeightCaution:Producthasnotbeenfullyvalidated