Resazurin-sodium-solution-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEResazurinsodium(solution)Cat.No.:HY-DY1051CASNo.:62758-13-8分⼦式:C₁₂H₆NNaO₄分⼦量:251.17作⽤靶点:Bacterial;FluorescentDye作⽤通路:Anti-infection;Others储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性Resazurinsodium(Diazoresorcinolsodium)(solution)⼀种⽆毒的、稳定的膜渗透性蓝⾊⾮荧光染料(有微弱的荧光)。Resazurinsodium作为氧化还原指⽰剂，在活细胞中能被还原成粉⾊的具有⾼度荧光的Resorufinsodium(Ex=530-560nm,Em=590nm)，从⽽⽤于细胞(⼈、动植物、细和真)活⼒、毒性、增殖、迁移和侵袭的检测。溶剂及浓度：⽆⽔：10mM体外研究Resazurinsodium(Diazoresorcinolsodium)iscommonlyusedtomeasurebacterialandeukaryoticcellviabilitythroughitsreductiontothefluorescentproductresorufin.Noviablebacteriaaredetected24hpost-inoculationfollowinginclusionofResazurinsodiuminTSBcculturesofF.tularensisLVSattheconcentrationof44μM.LoweringtheResazurinsodiumconcentrationtoaslittleas4.4μMstillresultsina10-foldreductioninviableF.tularensisLVScomparetogrowthmediumalone.BothResazurinsodiumtreatmentsresultinasignificantdecreaseinviableF.tularensisLVSbacteriaover22h.TreatmentwithResazurinsodiumsignificantlyreducesthenumberofviableF.tularensisLVSbacteriainHEK293cells22hpost-infection[1].Guidelines(Followingisourrecommendedprotocol.Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds).1.Solutionpreparation[2][3]1.1StocksolutionStorage:Storeat-20°Cor-80°Cindarkafteraliquoting.Avoidrepeatedfreezingandthawing.1.2PreparationofworkingsolutionDilutewithPBSorserum-freemedium.Thecorrespondingstocksolutioncanbedilutedaccordingtothe1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEactualsituation.NotethatifthesolventisDMSO,thecytotoxicityofDMSOmustbeconsidered,andasolventcontrolshouldbeprepared;ifthesolventispurewater,theworkingsolutionneedstobefilteredandsterilizedbeforeaddingcells.Note:Theworkingsolutionshouldbepreparedandusedimmediately.Keepitawayfromlight.2.Cellviability1.DefrosttheResazurinsodiumsolutionina37°Cwaterbath.2.Placethecellsona96-wellplate,andwashedbyPBS(avoidlight).3.RemovethePBSwash,thenadd100μL(1μg/mL)ofResazurinsodiumsolution.4.Placetheplate,intheincubatorfor30min(incubationtimedependsoncelltypeandcellnumber).5.MeasureResazurinsodiumfluorescence(Ex=530-560nm,Em=590nm)usingamicroplatereader.REFERENCES[1].SchmittDM,etal.TheuseofresazurinasanovelantimicrobialagentagainstFrancisellatularensis.FrontCellInfectMicrobiol.2013Dec6;3:93.[2].SilvaFSG,etal.DeterminationofMetabolicViabilityandCellMassUsingaTandemResazurin/SulforhodamineBAssay.CurrProtocToxicol.2016May4;68:2.24.1-2.24.15.[3].RampersadSN.MultipleapplicationsofAlamarBlueasanindicatorofmetabolicfunctionandcellularhealthincellviabilitybioassays.Sensors(Basel).2012;12(9):12347-60.McePdfHeightCaution:Producthasnotbeenfullyvalidate