Thioflavin-T-solution-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEThioflavinT(solution)Cat.No.:HY-DY1049CASNo.:2390-54-7分⼦式:C₁₇H₁₉ClN₂S分⼦量:318.86作⽤靶点:Amyloid-β作⽤通路:NeuronalSignaling储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性ThioflavinT(solution)⼀种阳离⼦Benzothiazole染料，在组织切⽚中与淀粉样蛋⽩结合后荧光强度增强。最⼤激发波长：~385nm(游离)；~450nm(结合)；最⼤发射波长：~445nm(游离)；~485nm(结合)。溶剂及浓度：⽆⽔：10mM体外研究ThioflavinTisafluorescentdyethatcanbindtoβ-fold-richstructuresandisoftenusedtomeasureproteinfibrillation.Oncebound,theemissionspectrumofThioflavinTwillbered-shiftedandthefluorescenceintensitywillincrease,whichcanalsobeusedtodetectfibrilformation.HereisareferenceprotocolforAβfibrilsdetection[2][3]:1.PrepareamixedsamplecontainingLMWAβ42(5.5μM),ThioflavinTsolution(20μM),andNaCl(100mM).2.Themixtureswerepreparedina96-wellhalf-areamicroplate.Sealthewellplateandplaceitinashakingincubatorat37°Cforindicatedtime.3.Shakefor20secondsbeforetesting.Settheexcitationlightto450nmandtheemissionlightto485nm.Readthefluorescencevalueusingamicroplatereader.REFERENCES[1].NakanoH,etal.Globular-shapedAβoligomershavediversemechanismsforpromotingAβaggregationswiththefacilitationoffibrilelongation.NeurobiolDis.2025Feb;205:106775.[2].MuramatsuD,etal.ALZ-801preventsamyloidβ-proteinassemblyandreducescytotoxicity:Apreclinicalexperimentalstudy.FASEBJ.2025Feb15;39(3):e70382.1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE[3].KhuranaR,etal.MechanismofthioflavinTbindingtoamyloidfibrils.JStructBiol.2005Sep;151(3):229-38.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-3792