β-catenin-IN-9-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEβ-catenin-IN-9Cat.No.:HY-179408CASNo.:3029462-00-5分⼦式:C₄₀H₅₈N₆OS分⼦量:670.99作⽤靶点:β-catenin;Apoptosis作⽤通路:StemCell/Wnt;Apoptosis储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性β-catenin-IN-9⼀种β-catenin抑制剂，诱导细胞凋亡(apoptosis)、细胞周期阻滞，并抑制结直肠癌细胞的迁移、侵袭和细胞上⽪-间质转化(EMT)。β-catenin-IN-9抑制β-catenin和vimentin的转录，并在蛋⽩质⽔平上显著抑制β-catenin。β-catenin-IN-9可⽤于结直肠癌的研究[1]。体外研究β-catenin-IN-9(compound10d)demonstratespotentcytotoxicactivityagainstColo-205cells,withanIC50valueof0.55μM[1].β-catenin-IN-9(0.55μM;48hours;Colo-205)demonstratespotentcytotoxicactivity,inducesapoptosis,andinhibitsmigrationandinvasion,inducescellcyclearrestincolorectalcancercells,leadingtoincreasedtotalcelldeathandS-phasecellaccumulation[1].β-catenin-IN-9(0.55μM;48hours;Colo-205)significantlyinhibitsβ-cateninproteinlevelsandincreasesvimentinproteinlevels[1].β-catenin-IN-9(0.55μM;48hours;Colo-205)significantlysuppressesgeneexpressionofβ-cateninandvimentin,andinducesE-cadherinexpression[1].CellMigrationAssay[1]CellLine:Colo-205Concentration:0.55μMIncubationTime:48hoursResult:Significantlydecreasedthepercentageofmigratedcellsby50%andthepercentageofinvasivecellsby66%,indicatingthatitnotonlyinhibitscellproliferationbutalso1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEsuppressesthemigrationandinvasioncapabilitiesofcolorectalcancercells.WesternBlotAnalysis[1]CellLine:Colo-205Concentration:0.55μMIncubationTime:48hoursResult:Significantlyinhibitedtheproteinlevelsofβ-cateninwithoutaffectingtheproteinlevelsofE-cadherin.Theproteinlevelofvimentinincreased,whichcontrastswithitseffectonvimentinmRNAlevel,indicatingpost-translationalregulation.RT-PCR[1]CellLine:Colo-205Concentration:0.55μMIncubationTime:48hoursResult:Significantlysuppressedthetranscriptionofβ-cateninandvimentin,whileinducingtheexpressionofE-cadherin,suggestingthatitinhibitstheepithelial-mesenchymaltransition(EMT)incolorectalcancercells.ApoptosisAnalysis[1]CellLine:Colo-205Concentration:0.55μMIncubationTime:48hoursResult:Thelateapoptosiswassignificantlyup-regulated14.8-fold,whilenecrosissignificantlyshoweda16-foldincrease.CellCycleAnalysis[1]CellLine:Colo-205Concentration:0.55μMIncubationTime:48hoursResult:LedtocellcyclearrestatS-phaseincolorectalcancercells.REFERENCES2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE[1].AbdelmegeedH,etal.Anovel1,2,4-triazolederivativeinhibitsepithelial-mesenchymaltransitioninmetastaticcolorectalcancerviaβ-cateninsuppression.EurJMedChem.2026;302(Pt1):118279.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedic