（正式版）DB34∕T 4223-2022 《黄缘闭壳龟　》

34Yellow-marginedBoxTurI本文件按照GB/T1.1—2020《标准化工作导则第1部分：标准化文件的结构和起草规则》的规定请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。增、方国侠、沈保平、高凌、徐丽、彭步旭、鲍鸣、张四刚1黄缘闭壳龟征、生长和繁殖特性、遗传学特征、检测方法GB/T18654.2养殖鱼类种质检GB/T18654.12养殖鱼类种质检验GB/T18654.15养殖鱼类种质检验第4名称与分类黄缘闭壳龟（CuoraflavomarginataGray，1863）。5.1.1外形体呈椭圆形，前部略窄、后部略宽。头光滑无鳞，鼓膜圆而清晰，头部背面呈橄榄色，两眼后各2具一条金黄色条纹，颈部呈浅橘红色。背甲与背甲间有甲桥连接，腹甲前后两部分向上5.1.2.1背甲椎盾：5枚。肋盾：8枚，左右各4枚。缘盾：22枚，左右各11枚。臀盾：2枚，左右各1枚。35.1.2.25.1.2.3g，背甲长104.6mm～121.2mm，年龄4+～6+。各注：各可量性状说明参见GB/T34727长，胃分化明显，十二指肠上附有条形白色胰脏，无盲肠。雌雄异体，雄性睾丸1对，淡白色，卵圆形，位于肾脏前端内侧，后端为输精管，通泄殖腔，阴茎位于尾基部的泄殖孔内；雌体卵巢1对，位46.1生长背甲宽自然条件下，雌龟为6性成熟，雄龟是5性成熟。每年5月～8月产卵，5月中旬～6月上旬为产卵高峰期。一年多次产卵，一般每年产卵1次～4次。每只雌龟每年产卵1枚/次～4枚/次。7.1.2染色体核型见图3,染色体组型见图4。5黄缘闭壳龟线粒体16SrRNA基因片段的碱基序列为1596bp,种内K2P遗传距离为0.0024,小于2%,标准序列见附录C。69判定规则7Wt1=12.73e0.476（r=0.9843）·················································Lt=45.47T0.466（r=0.9944）········································Wt2=34.81T0.461（r=0.9939）·····················································(A.3)Wt2——t齢时龟背甲宽，单位为毫米（mmWt1=Lt3.018（r=0.9899）··························································(Wt1——t齢时龟体重，单位为克（gLt=0.82Wt21.191（r=0.9925）·············································Wt2——t齢时龟背甲宽，单位为毫米（mm8(规范性)取5μL扩增产物上样到1.0%的琼脂糖凝胶进行电泳分离(见图B.1),9黄缘闭壳龟线粒体16SrRNA基因标准序列（1596b1GCAAGAACCTAGCCCAACCAAAATAAAAAACTTAACAAACAAAACTATCC51CACCAAAACCAACCAAAACATTCTCACCATCCTAGTATAGGCGATAGAAA101AGACAGTTGGAGCAATAGAGATAGTACCGCAAGGGAAAGATGAAAAACAA151TGAAATATTCACTAAGCCATAAAAAGCAAAGATTAATCCTTATACCTTTT201GCATCATGATTTAGCTAGCATATTCAAGCAAAGAGCCCTAAAGTATGAAA251CCCCGAAACTAAGTGAGCTACTTAAAGGCAGCCACACCAAGGCTAAATCC301GTCTCTGTGGCAAAAGAGTGGAAAGACCTGTAAGTAGAGGTGAAAAGCCT351AACGAACTTAGTGATAGCTGGTTGCTCAATAAAAGAATATAAGTTCAACC401TTAAACCTTCTAAAAACAACCTAAAGTATAAAAGAAAAGTTTAAGATTTA451TTCAATTGGGGTACAGCCCAATTGAAAAAGGATACAACCAATAACGGAGG501ACAAAACCCCAAAAATAACAACCGTAGGCCTTAAAGCAGCCACCAACTAA551GAAAGCGTCAAAGCCCACCCGCTATTAAATAGTAACAAAAAATTTTTTCC601CCTAATAATATTGAGCTACTCCATTTAAATAGAGGAATTAATGCTAAAAT651GAGTAACAAGAAGACAAAACTTCTCTAATACGCTAGCTTAAGTCATAACA701GATAAACTACTGACTATTAACATCCATCACTATAAAACAACAACATTTTA751AATCACCCTATAGACCTAACTGTTAACCCAACACAGGAGCGTATACAAGA801AAGATTAAAATTTGTAAAAGGAACTAGGCAAATAAACGAGCCCGACTGTT851TACCAAAAACATAGCCCCTAGCAACACAAGTATTAGGGGTAATGCCTGCC901CAGTGACACTGTTAAACGGCCGCGGTATCCTAACCGTGCAAAGGTAGCGT951AATCACTTGTCTTTTAAATAAAGACTAGAATGAATGGCCAAACGAGGTTC1001TACCTGTCTCTTACAAATAATCAGTGAAATTGATCTTCCTGTGCAAAAGC1051AGGAATAACATTATAAGACGAGAAGACCCTGTGGAACTTCAAATACAAAT1101CAACTATTATCAACACCCACCTACGGGTCCACATCAAACTAGCATCTGAC1151TTATATTTTCGGTTGGGGCGACCTCGGAGTAAAATAAAACCTCCGAAAAA1201GAATTTCCTTTTAAACCTAGACCTACAGCCCACAGTGCTTCCAGCAAAAC1251GATCCAATATACTTGATCAACGAACCAAGCTACCCCAGGGATAACAGCGC1301AATCCCATCCTAGAGTTCCTATCGACGATGGAGTTTACGACCTCGATGTT1351GGATCAGGACATCCTGATGGTGCAACTGCTATCAAGGGTTCGTTTGTTCA1401ACGATTAACAGTCCTACGTGATCTGAGTTCAGACCGGAGCAATCCAGGTC1451GGTT