免疫组库测序技术介绍

1、immuno-seq: exploring the diversity of immune system,jiankui he south university of science and technology of china(sustc), shenzhen, china,the diversity of invaders . . .,bacteria viruses parasites such as fungi, protista, 5(171):171,接受流感疫苗后,immuno-seq,immuno-seq技术可以观察和分析t细胞和b细胞，达到前所未有的深度和特异性。在疾病监控

2、，抗体生产，疫苗研究，健康体检等领域有广泛的应用。,现在临床上有许多种检测可以监控你的健康状况， 但是却没有一种被广泛接受的方法可以检测你免疫系统的各个方面。 我们怎么可以得知一个人的免疫系统是很强健还是很虚弱？ 在人们受哮喘，过敏，关节炎折磨时，如何量化免疫系统的错误？ 斯坦福大学的一个研究团队在开发这样的检测方面迈出了第一步， 他们的方法是：给免疫系统“拍照”。,there are numerous tests to gauge the health of your heart. but no such widely accepted test exists for the many pa

3、rts of the immune system. how can we tell if the immune system is strong or weak? or quantify how badly its malfunctioning when we suffer from asthma, allergies, or arthritis?,-francis collins, nih director 2013年2月13日,immune response to flu vaccine,day 0,day 7,day 28,flu vaccine: tiv & laiv,collect

4、5ml blood,experiment flow,broad vdj gene segment usage pattern,v,d,j,a snapshot of immune system,day 7, after vaccination. elderly subject,we amplified antibody sequences from single sorted plasma b cell . we then expressed monoclonal antibodies according to these sequences and verified their bindin

5、g to each of the three virus strains in the vaccine,immune reperotire is a dynamics system,day 0,day 7,day 28,vaccine specific antibody lineage 13% of total sequences,vaccine specific antibody lineage 0.01% of total sequences,elderly subject,reduced antibody diversity in elderly,age 8-17,age 18-32,a

6、ge 70-100,mutation,each data point represent a lineage,comparison of 3 subjects,sequences in a lineage,reduced antibody diversity in elderly,70100 year old volunteers had a significantly less diverse collection of antibodies than the younger volunteers. that is, as people aged, their immune system h

7、ad a more limited repertoire of antibodies to offer,lab members,postdoc: zhoufang li technician: luwen ning meng zhang students: jiajie guo yin tong ying xu peng li yao chen ronghai cheng,collaborators: pinghua liu (boston u), hengye man (boston u) zhigang liu (shenzhen u), zhanhui wang (smu), zhiming cai (2nd hospital of shenzhen), xiao liu (bgi) xiao