新闻翻译：香菇基因组序列信息 中英文

1、边银丙教授团队完整公布香菇基因组序列信息Prof. Bian Yinbing Team Completely Releases GenomeSequence of Lentinula edodes (Shiitake)2016-09-01 11:36 植物科学技术学院南湖网讯（通讯员 向姑）近日，我校边银丙教授研究团队在 PLoS ONE（国家公共科学图书馆 综合）上在线发表了题为 “Genome sequence of the edible cultivated mushroom Lentinula edodes (shiitake) reveals insights into Lignoc

2、ellulose degradation”的研究论文。这是中国独立完成并完整公布的香菇基因组参考序列和注释信息，初步明确了香菇降解木质纤维素的遗传物质基础，对提高香菇的栽培基质转化利用效率和开发新型栽培基质具有重要的参考价值。博士生陈连福为论文第一作者，边银丙教授为通讯作者。本研究得到国家自然科学基金资助。Recently, Prof. Bian Yinbing research team in our university online published an article titled Genome sequence of the edible cultivated mushroom

3、Lentinula edodes (shiitake) reveals insights into Lignocellulose degradation on PLoS ONE. It marks that Chinas independent release of genome reference sequence and annotation of L. edodes which preliminarily reveals insights into the genetic material basis of L. edodes lignocellulose degradation. Th

4、e article offers a better understanding of improving the transforming and using efficiency of L. edodes cultivation medium and developing new cultivation medium. Ph.D candidate Chen Lianfu is the first author, and Prof. Bian Yinbing is the corresponding author. The study was supported by the Nationa

5、l Natural Science Foundation of China.香菇是我国产量最高、出口量最大和栽培历史最悠久的食用菌，在全国各地广泛栽培，被称为“国菇”。由于其具有独特的风味和较高的营养保健价值，深受亚洲各国消费者喜爱。目前香菇人工栽培需要以木屑为主要原料，对森林资源依赖性较高，开发秸秆类栽培基质十分必要。L. edodes is a kind of edible fungus with longest cultivation history and largest yield and exports in China. Its known as National Mushroo

6、m as it is widely cultivated across the country. L. edodes possesses unique aroma components, rich nutrition and medicinal value, which render shiitake overwhelmingly popular with consumers in Asian countries. However, L. edodes cultivation is mostly dependent on forests resources as its main cultiv

7、ation material is wood sawdust, so it is very necessary to develop cultivation medium like straw.本研究选用香菇单核体菌株 W1-26 进行了基因组测序，完成了基因组组装、注释和分析，预测香菇基因组大小为 41.8 Mb，包含约 14,889 个基因。通过基因注释和功能预测表明，香菇基因组中包含 102 个参与木质纤维素降解过程的蛋白酶类基因，明确了木质纤维素降解相关酶基因种类构成及基因数量，进一步结合转录组数据发现，2 个纤维素酶基因和 16 个转录因子基因在纤维素培养基质中表达量有明显提升，并以

8、此为基础正在进一步开展香菇降解纤维素的分子机制研究。同时开展了香菇交配型 A 位点和 B 位点的结构分析。还鉴定出与香菇风味物质代谢相关的 7 个-谷酰胺转酞酶（ggt）基因和 5 个半胱氨酸脱硫酶（csl）基因，这类基因数量远多于其它真菌物种。另外系统发育分析表明，香菇与裸脚菇 c 亲缘关系最近。The team completed genome assembly, annotation and analysis by selecting Shiitake monokaryotic strain W1-26 for genome sequencing. The team reported 4

9、1.8Mb genome, encoding 14,889 predicted genes or so. Genes annotation and its function predication shows that sL. edodes genome consists of 102 protease genes involving in the degradation of ligncellulose, and defines the species composition and quantities of enzyme genes in the degradation of lignc

10、ellulose. Due to the discovery of significant increase of the expression level of two cellulase genes and sixteen transcription-factor genes in cellulase cultivation medium by combing with transcriptome data, both molecule mechanism research on L. edodes degraded cellulose and the structure analysis

11、 on L. edodes mat A and matB loci are being further conducted. Furthermore, seven -ggt genes and five csl genes relating to metabolic shiitake-aroma components, far more than other fungus species, have been verified. In addition, phylogeny analysis indicates that L. edodes has the closest genetic re

12、lationship with gymnopus luxurians.香菇基因组信息的公布为香菇功能基因组研究提供了较好的生物信息学平台并为培育高产优质香菇新品种、提高栽培基质利用效率和开发新型栽培基质等方面提供了参考信息，也有利于进一步开展食用菌相关领域的科学研究工作。The publication of L. edodes genome provides better bioinformatic platform for functional genome research of L. edodes. It also offers a better understanding of cultivating new type L. edodes with high yield and good quality, and improving utiliz