IWP-4 _Wnt 抑制剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEIWP-4Cat. No.: HY-12879CAS No.: 686772-17-8分式: CHNOS分量: 496.62作靶点: Wnt作通路: Stem Cell/Wnt储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 5 mg/mL (10.07 mM; Need ultrasonic and warming)Mass S

2、olvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 2.0136 mL 10.0681 mL 20.1361 mL5 mM 0.4027 mL 2.0136 mL 4.0272 mL10 mM 0.2014 mL 1.0068 mL 2.0136 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 IWP-4种分 Wnt 抑制剂，其 IC50 值为 25 nM。IC50 & Target IC50: 25 nM (Wnt) 1体外研究IWP-4 is a small mo

3、lecule Wnt inhibitor with an IC50 of 25 nM. IWP-4 induces the expression of cardiacmarkers, including cardiac troponin I (CTNI) and cardiac myosin heavy chain bright cells (MYHhi +). IWP-4also results in the appearance of beating foci (0.440.10 SEM beats per second), which is absent in all1/2 Master

4、 of Small Molecules 您边的抑制剂师www.MedChemEcultures not receiving IWP-4. Further, flow cytometric analysis shows that there are significantly more MYHlo+ cells in IWP-4 treated cultures (P 1. Mesenchymal precursor cells (MPCs) treated with IWP-4 show nosignificant changes in the expression of AXIN2, CTN

5、NB1 and GSK3B as compare to osteogenic mediumalone on day 7, but MPCs treated with IWP-4 express elevates levels of DKK1 and GSK3 on day 21. IWP-4also causes a significant down regulation of SPARC and COL1A1 2.PROTOCOLCell Assay 1 hESC cultures are obtained in mTeSR-1 medium and expanded with daily

6、medium exchange until coloniesreach the desired level of conuence (70% to 80%). At this time (marked day 0), mTeSR-1 is replaced witha basal medium comprised of RPMI 1640 medium supplemented with 2% B27 supplement and 1%penicillin/streptomycin. 20 ng/mL BMP-4 and/or 6 ng/mL activin A are added to th

7、e basal medium forprimitive streak induction, and exchanged daily until day 3. Then, basal media with or without 5 mM IWP-4 isadded to the cells and exchanged every 2 days dimethyl sulfoxide (DMSO) at the same concentration isused as a vehicle control until day 15, after which basal medium is suppli

8、ed every 2 days 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Hudson J, et al. Primitive cardiac cells from human embryonic stem cells. Stem Cells Dev. 2012 Jun 10;21(9):1513-23.2. Frith JE, et al. Microbioreactor array screening of Wnt modulators and microenvironmental factors in osteogenic differentiation ofmesenchymal progenitor cells. PLoS One. 2013 Dec 23;8(12):e82931.McePdfHeightCaution: Product has not been fully validated for medical appli