专业医院03.培训lovo介绍中文版

1、 Copyright Fresenius Kabi AGJune 9, 2017FORTUNE GLOBAL 500 ！Fresenius Kabi Deutschland GmbHBad Homburg v. d. H., GermanyCOM.TECVersatile Multi-Procedural Apheresis PlatformLovo 在 多种细胞处理技术，多环节广泛应用Lovo用途自动化细胞高效去除培养液，上清液，药物，杂质，血小板等保存液，液替换/置换细胞浓缩，产品体积压缩纯化用于科研，生物制品生产加工，制药，生物技术等广泛领域在多环节，广泛使用Lovo特性业界独家使用“旋

2、转膜滤过” 【Spinning Membrane Filtration】 ，非离心式提取原理（业界独家）温和处理技术，减少细胞损伤，不但没有高速离心造成的细胞损伤，“微旋流”保护细胞（业界独家）细胞始终“始终保持悬浮”状态，无需再悬浮 离散处理（业界独家）自动“多高速加滤过技术”，首次90%纯度产品，2次99%，3次99.9%，4次99.99%. （业界独家）分离，高效，快速，自动化，大幅减少人工干预，无需人工看管（业界最优）Lovo在多种细胞处理技术，多环节广泛应用T-Cell with CARCAR-T带有特异性肿瘤识别功能的T淋巴细胞添加 CARCAR（chimeric antigen

3、receptor）嵌合抗原受体一种能够识别特异性肿瘤抗原的特殊受体COM.TECMouclear CellVersatile Multi-Procedural Apheresis Platform细胞产品洗涤纯化产品分类国际又名“硬分离清洗技术 ”国际又名“软分离清洗技术 ”6COM.TECVersatile Multi-Procedural Apheresis PlatformFresenius Cell Washing Concept旋转式滤膜系统用于分离细胞及基质液体，杂质，学校板等 Spinning membrane technology used inplasmapheresis磁驱

4、动转子滤过法清除基质液体，而非离心分离法，因而没有细胞凝聚效应 Remove supernatant by filtration; no初产品pelletization膜式分离按照分子大小区隔，去除所有小于4um 细胞基质液及杂质 Membra“size discrimination”; exlection allows forle:Generally, retain cell content 4um; Remove cellcontent 4um后浓缩细胞出口连续式处理技术，没有容量大小限制 Automatedpros without volume limiionsFenwal Overvi

5、ew Presen ion | 6/9/20177洗涤废液出口Polycarbonate Membrane Filtration Utilizes Cell Size DiscriminationPolycarbonate Membrane4.0 m pore size10 m thicknessEtched, cylindrical poreRetains WBCs and someRBCsApproximate Scale:SEM of Polycarbonate Membrane= RBC= WBC=PLTFenwal Overview Presen ion | 6/9/20178NOV

6、EL CELL WASHING DEVICE USINGSPINNINGMEMBRANE FILTRATION独家“旋转膜滤过”技术 带来的独特优势Improve mouclear cell recovery单个核细胞恢复率Minimizing Cell loss大大降低细胞损伤丢失Avoid cell damage caused by high-speed centrifugation完全杜绝离心技术导致的细胞损伤-Pelletizing to Cells无离心技术造成的细胞eed Cell Resuspen无需细胞离散在悬浮处理效应Speed ! Automated !高效，全自动Lovo

7、 is the only cell prosing system usingspinning membrane technology !June 9, 2017 Copyright Fresenius Kabi AGKey Profile of Lovo10Lovo 连续式处理技术，高效，不受液体量限制 Lovo 细胞损伤小，细胞恢复率高 Lovo细胞保持悬浮状态，无需再悬浮处理Lovo 多Lovo全自动，无需人工干预，避免看管技术加滤过Automate with LOVOSee！Lovo 连续式处理技术，高效，不受液体量限制 Copyright Fresenius Kabi AGJune 9

8、, 2017Procedure Start:500 mL Source Product500 mL Source Product Copyright Fresenius Kabi AGJune 9, 2017FiltrateWash MediaWash Cycle: Concentration Phase50 mL In-pro (Concentrated)s Copyright Fresenius Kabi AGJune 9, 2017FiltrateWash Cycle: Dilution Phase500 mL In-pro(Diluted)s Copyright Fresenius K

9、abi AGJune 9, 2017FiltrateWash MediaUser-Defined FinalProduct VolumeUser-defined Product Volume Copyright Fresenius Kabi AGJune 9, 2017FiltratePump Speeds Combined with Fluid Management Results in Fast ProsingSle Prosing Times*Approximate Source VolumeFinalVolumeWashoutProsingCell TypeTimeFresh PBMC

10、s200 mL150 mL99%7 minsCryop240 mL150 mL99%15 minsPBMCsCD4+ T-Cells2000 mL150 mL99%30 minsJurkat Cells1200 mL100 mL99%25 mins* Data areended to be directional only. Multiplevariables includingcellconcentration can effect prosing times. Data on File. Copyright Fresenius Kabi AGJune 9, 2017Key Profile

11、of LovoLovo 连续式处理技术，高效，不受液体量限制Lovo细胞保持悬浮状态，无需再悬浮处理Lovo 多加滤过技术17Lovo全自动，无需人工干预，避免看管Lovo 细胞损伤小，细胞恢复率高Automate with LOVO独家“旋转膜滤过”技术 细胞保护的独特优势细胞损伤小细胞回收率高细胞活性保持佳无细胞，反而更充分悬浮离散WBCRBCPLTD ev i c e W a l lM em b r a n e F i l t er LOVO 细胞保护之 MNC 恢复率 Data Presenion at ISCT Copyright Fresenius Kabi AGWashed Pr

12、oduct % RecoverySpinning Membranet-Wash MNCRecovery (n=22)100%90%80%70%WBC60%RBC50%40%PLT30%Sup20%10%0%Lovo Prototype Instrument:High supernatant removal with good TNC recovery1t-Wash RecoveriesAdditionalt-Prosing Detail100%Fresh PBMC Products92% TNC Recovery92.3%80%74.4%86% Plaet Removal60%25% RBC

13、Removal99% Supernatant Removal7 min (200 mL to 150 mL)40%20%14.2%(prosing only)1.3%0%TNCRBCPlaetpernatantt-wash cell recoveries from a 2 wash cycle protocol using 10:1 concentrationratio. Recoveries are shown as percent of source cell suspenwashed product.recovered in1Presented at the 19nnualernatio

14、nal Society for Cell Therapies Meeting, April 2013. Dollected using prototype instrument.Wegener C, Heber C, Min K (2013). Novel Cell Washing Device Using Spinning Membrane Filtration. Cytotherapy. 15(4) S27. Abstract 86. Copyright Fresenius Kabi AGJune 9, 2017Lovo Prototype Instrument:Viabilities b

15、etn washed and unwashed cells not different13-Day Lymphocyte Apoptosis Levels(n=5)Day-3 Lymphocyte Proliferation(n=4)908070605040302010014.0012.0010.008.006.004.002.000.000123DayUnwashedWashedUnwashedWashed1Presented at the 19nnualernational Society for Cell Therapies Meeting, April 2013. Dollected

16、using prototype instrument.Wegener C, Heber C, Min K (2013). Novel Cell Washing Device Using Spinning Membrane Filtration. Cytotherapy. 15(4) S27. Abstract 86. Copyright Fresenius Kabi AGJune 9, 2017% Annexin V+% Lymphocyte ProliferationKey Profile of LovoLovo 连续式处理技术，高效，不受液体量限制Lovo 细胞损伤小，细胞恢复率高Lovo

17、 多加滤过技术22Lovo全自动，无需人工干预，避免看管Lovo细胞保持悬浮状态，无需再悬浮处理Automate with LOVO旋转滤过膜技术细胞保持“悬浮离散状态”，并在“微旋流”中受到保护旋转技术转膜外侧产生“微旋流”，杂质在外被“吸除”，而“白细胞/单个核细胞”在最内侧包绕而“受到保护”，并一直处于离散悬浮状态Typical Cell Wash传统离心式细胞纯化方式 细胞经过离心压缩，还需再悬浮处理！传统离心式细胞纯化方式而Lovo完全不需要!Pelletize细胞离心压缩Decant弃除分离液Manual prosRequires centrifugationNot conveni

18、ent for large volumesResuspend再离散悬浮Fenwal Overview Presen ion | 6/9/201724Key Profile of LovoLovo 连续式处理技术，高效，不受液体量限制Lovo 细胞损伤小，细胞恢复率高Lovo细胞保持悬浮状态，无需再悬浮处理Lovo 多加滤过技术 25Lovo全自动，无需人工干预，避免看管Automate with LOVOLovo 多加滤过技术纯度的产品 获得指数级Overview of a 2-Wash Cycle Pros10:1Concentration1:10Dilution1Supernatant C

19、oncentration Supernatant Volume Wash Media Volume Cell ConcentrationNumber of Cells100%100 mL0 mL 1x 50100%10 mL0 mL 10 x 5010%90 mL1x 5010:1Concentration1:10Dilution2Supernatant Concentration Supernatant Volume Wash Media Volume Cell ConcentrationNumber of Cells10%90 mL1x 501%9 mL10 x 501%99 mL1x 5

20、010:1 Concentration特有“多加滤过技术” 首次90%清除，2次99%，3次99.9%，4次99.99%.Number of Wash Cycles99% supernatant reduction！ in under 7 minutes！ in a 200 mL source product“多加滤过技术”为Fresenius Lovo 特有传统的离心浓缩技术，由于离心力造成细胞损伤，无法多重处理.*Lovo is not able to perform a 1-cycle wash. Washouts for 1-cycle are included for illustr

21、ative pures only. Copyright Fresenius Kabi AGJune 9, 2017Most commonly usedwashoutsConcentration1*23456Ratio10:190%99%99.99%99.999%99.9999%99.9%5:180%96%99.6%99.96%99.996%99.9996%2:150%75%99.5%99.95%99.995%99.9995%Key Profile of LovoLovo 连续式处理技术，高效，不受液体量限制Lovo 细胞损伤小，细胞恢复率高Lovo细胞保持悬浮状态，无需再悬浮处理Lovo 多加

22、滤过技术Lovo全自动，无需人工干预，避免看管29Automate with LOVO技术原理决定使用便捷便利操作，无需过多人工看管操作安装管路，设定好几项简单参数后，无需人工看管！液晶触摸控制屏全自动化处理管路安装极简 Copyright Fresenius Kabi AGJune 9, 2017NOVEL CELL WASHING DEVICE USINGSPINNINGMEMBRANE FILTRATION独家“旋转膜滤过”技术 带来的独特优势Improve mouclear cell recovery单个核细胞恢复率Minimizing Cell loss大大降低细胞损伤丢失Avoid

23、 cell damage caused by high-speed centrifugation完全杜绝离心技术导致的细胞损伤-Pelletizing to Cells无离心技术造成的细胞eed Cell Resuspen无需细胞离散在悬浮处理效应Speed ! Automated !高效，全自动Lovo is the only cell prosing system usingspinning membrane technology !June 9, 2017 Copyright Fresenius Kabi AGLOVO Cell Prosing SystemAutomated Cell ProWhite Blood Cells:sing forSupernatant or Culture MediaExchangeSupernatant Volume Reduction/ CellConcentrationReduction of PlaetsLovo is For Laboratory Use Onlyand does nove 510(k) clearanceEnables closed system prosingLovo is