组织胚胎学blood and hemopoiesis 课件

组织胚胎学课件

七年制英文医学班专用中国医科大学基础医学院组胚—英文教学组组织胚胎学课件

七年制英文医学班专用中1Chapter5

BloodandHemopoiesis

Chapter5

BloodandHemopoie21.

Components:---formedelements:45%redbloodcell-erythrocytewhitebloodcell-leukocyteplatelets---plasma:55%,PH7.3-7.4,90%watercontain:plasmaprotein(albumin,globulin,fibrinogen),lipidprotein,enzymes,hormone,vitamin,inorganicsaltandproductsofmetabolism*serum:isplasmainwhichthefibrinogenhasbeenremovedbyclotting1.

Components:3Bloodsmear:WrightorGiemsastainmethodsBloodsmear:WrightorGiemsa41.

Cells

1.

Cells

51)erythrocyte,redbloodcell---normalnumber:(4.2-5.5)X1012/Linmale(3.5-5.0)X1012/Linfemale---structure:biconcavediscsinshape,7.5-8.5umindiameter,1-2umthicknonucleusandorganellafilledwithhemoglobin,Hb:about1/3-normalnumber:120-150g/Linmale105-135g/Linfemale-combineandtransferO2andCO21)erythrocyte,redbloodcell6erythrocytemembraneskeleton:-changeshape-maincomponentisspectrinandactinbloodtypeantigen:A,B:-membraneprotein-ABObloodtype-hemolysis→erythrocyteghost*anemia:RBC<3.0X1012/LorHb<100g/L组织胚胎学bloodandhemopoiesis课件7*reticulocyte:0.5-1%oftotalerythrocytepopulationor3-6%innaonateimmaturecellremainedribosome-delicatenetworkstructurewhenstainedwithbrilliantcresylbluewilldisappearafter1-3days---lifespan:120days组织胚胎学bloodandhemopoiesis课件82)leukocyte---agroupoflargecellswithnucleus---involveindefenseandimmunereaction---normalnumber:(4.0-10)X109/L---classification:granulocytes:/neutrophil/eosinophil/basophilagranulocytes:/lympocyte/monocyte2)leukocyte9

①

neutrophil:

---normalpercentage:50-70%oftotalleukocytepopulation---structure:LM:round,10-12umindiameterrob-likedorpolymorphousnucleus,2-5lobesfineneutrophilicgranules-pinkcytoplasm组织胚胎学bloodandhemopoiesis课件10EM:specificgranule:80%small,0.3-0.4umovoidorirregularinshapecontain:lysozyme,phagocytin(defensin)

azurophilicgranule:20%large,roundorovoid,0.6-0.7umelectrondenselysosome:acidphosphatase,peroxidase,acidichydrolase组织胚胎学bloodandhemopoiesis课件11---function:phagocytosebacterium:specificg.-killBazurophilicg.-digestB---lifespan:inblood6-8hours,inCT2-3days---function:phagocytosebacte12

②

eosinophil:---normalpercentage:0.5-3%oftotalleukocytepopulation---structure:LM:round,10-15umindiameter2lobesnucleusroughbrightredgranules-acidophilicgranules组织胚胎学bloodandhemopoiesis课件13EM:granules:roundorovoidwithcube-likedelectrondensecrystalcontain:-ACPase-histaminase-peroxidase---function:counteracttheinfectionofparasitereduceallergicreaction---lifespan:inblood6-8hour,inCT8-12daysEM:granules:14③

basophil:---normalpercentage:0-1%oftotalleukocytepopulation---structure:LM:less,round10-12umlobe,“S”shapedorirregularnucleusbasophilicg.:containsheparin,histamineandacidophilchemotacticfactorcytoplasm:containleukotriene---function:involveinallergicreaction---lifespan:10-15days③

basophil:15④

monocyte:---normalpercentage:3-8%oftotalleukocytepopulation---structure:LM:largeround,14-20umnucleusappearaskidney,horse-shoeorovoidinshapecytoplasm:gray-blueincolor,containazurophilicg.④

monocyte:16EM:azurophilicgranule:lysosome-containperoxidase,ACPase,non-spacialesteraseandlysozyme---function:activelymobileandchemotaxis---lifespan:inblood1-5days,inCTwhichbecomeintomacrophage-mononuclearphagocyticsystem,MPS组织胚胎学bloodandhemopoiesis课件17

⑤lymphocyte:---normalpercentage:20-30%oftotalleukocytepopulation---structure:LM:round,smallLC6-8um,medium-sizedLC9-12um,largeLC13-20umindiameterroundnucleuswithindentation,chromatinappearsasspot-likedandelectron-densecytoplasm:/basophilic:brightblueincolor/less/azurophilicgranule⑤lymphocyte:18EM:/freeribosome/mito./RER---classification:TC:thymusdependentlymphocyte,75%,involveincellularimmunereactionandregulateimmuneresponseBC:bonemarrowdependentlymphocyte,10-15%,becomeintoplasmacell,involveinhumoralimmuneresponselargegranularcell:KC(killercell)andNKC(naturekillercell),10%---function:involveinimmuneresponse组织胚胎学bloodandhemopoiesis课件193)bloodplatelet:cytoplasmicfragmentofmegakaryocyteinbonemarrow---normalnumber:100-300X109/L---structure:LM:disc-liked,2-4um→irregularinshapeStimulationingroupsGranulomerehyalomere3)bloodplatelet:cytoplasmic20EM:/cellcoat:glycosaminoglycanandglycoprotein/tubularsystems:openingtubulesystem-increasingtheexchangearea,facilitatetheintakingandreleasingdensetubulesystem-SER,collectingCa2+andsynthesizingprostaglandin/granules:specificgranule:mediateelectrondensity,withdensecore,containsclottingfactor,fibrinogen,acidichydrolasedensegranule:electrondense,containsserotonin(5-hydroxytryptamine),ADP,ATP,Ca2+andadrenalinEM:21组织胚胎学bloodandhemopoiesis课件22---function:involveinclottingandstoppingthebleed:-aggregation-releasecomponentsofgranule,makethrombinogenbecomeintothrombin,thenlatermakefibrinogenbecomeintofibrinprotectingendotheliumandtakepartinrepairingofendothelium---function:233.

Bonemarrowandhemopoiesis1)Placesforhemopoiesisandstructureofbonemarrow①Placesforhemopoiesis:yalksac(3W)→liver(6W)→spleen(4M)→bonemarrow3.

Bonemarrowandhe24②Structureofbonemarrow:redbonemarrow---hemopoietictissue:reticularT.hemopoieticcell,macrophage,fibroblast,lipid-ladencellandmesenchymalcell---sinusoid:cap.组织胚胎学bloodandhemopoiesis课件25*hemopoieticinductivemicroenvironment(HIM):

Theenvironmentforhemopoieticcellgrowthanddevelopment,bonemarrowHIMincludesNofbonemarrow,BV,fiber,extracellularGSandhemopoieticstromalcells*hemopoieticinductivemicroen26*stromalcells:---includingreticularcell,fibroblast,macrophage,endothelialcellandlipid-ladencell---function:supportingsecretecellularfactors,regulatetheproliferationanddifferentiatingofhemopoieticcells*stromalcells:272)Hemopoieticstemcellandhemopoieticprogenitorcell①hemopoieticstemcell(HSC):multipotentialstemcell---originatedyalksac→redbonemarrowconstitutesabout0.5%oftotalbonemarrowcells---similartosmallLC---feature:strongpotentialtoproliferation,butgenerallyinGostillstatemultidifferentiatedabilityabilitytocopyitself:keepcertainnumber2)Hemopoieticstemcellandh28②hemopoieticprogenitorcell(HPC):committedstemcell---originatedfromhemopoieticstemcell---formingcolonyformingunit(CFU)undertheregulateofcolonystimutatingfactor(CSF):CFU-GEMMBFU-E,CFU-ECFU-GMCFU-MKCFU-TL,CFU-BL②hemopoieticprogenitorcell(H293)Generalregulationofhemopoieticprocesses:---mainsteps:promordialstageimmaturestagematurestage3)Generalregulationofhemop30---regulations:sizeofcellbecomesmallerandsmallerNucleusbecomesmallerandsmallerordisappearschromatin:fromfineandloosetoroughanddensecytoplasm:-moreandmore-basophilic:weakerandweaker-specificgranule:moreandmoreabilitytodivision:fromhavetono,butkeepthepotentials---regulations:31组织胚胎学课件

七年制英文医学班专用中国医科大学基础医学院组胚—英文教学组组织胚胎学课件

七年制英文医学班专用中32Chapter5

BloodandHemopoiesis

Chapter5

BloodandHemopoie331.

Components:---formedelements:45%redbloodcell-erythrocytewhitebloodcell-leukocyteplatelets---plasma:55%,PH7.3-7.4,90%watercontain:plasmaprotein(albumin,globulin,fibrinogen),lipidprotein,enzymes,hormone,vitamin,inorganicsaltandproductsofmetabolism*serum:isplasmainwhichthefibrinogenhasbeenremovedbyclotting1.

Components:34Bloodsmear:WrightorGiemsastainmethodsBloodsmear:WrightorGiemsa351.

Cells

1.

Cells

361)erythrocyte,redbloodcell---normalnumber:(4.2-5.5)X1012/Linmale(3.5-5.0)X1012/Linfemale---structure:biconcavediscsinshape,7.5-8.5umindiameter,1-2umthicknonucleusandorganellafilledwithhemoglobin,Hb:about1/3-normalnumber:120-150g/Linmale105-135g/Linfemale-combineandtransferO2andCO21)erythrocyte,redbloodcell37erythrocytemembraneskeleton:-changeshape-maincomponentisspectrinandactinbloodtypeantigen:A,B:-membraneprotein-ABObloodtype-hemolysis→erythrocyteghost*anemia:RBC<3.0X1012/LorHb<100g/L组织胚胎学bloodandhemopoiesis课件38*reticulocyte:0.5-1%oftotalerythrocytepopulationor3-6%innaonateimmaturecellremainedribosome-delicatenetworkstructurewhenstainedwithbrilliantcresylbluewilldisappearafter1-3days---lifespan:120days组织胚胎学bloodandhemopoiesis课件392)leukocyte---agroupoflargecellswithnucleus---involveindefenseandimmunereaction---normalnumber:(4.0-10)X109/L---classification:granulocytes:/neutrophil/eosinophil/basophilagranulocytes:/lympocyte/monocyte2)leukocyte40

①

neutrophil:

---normalpercentage:50-70%oftotalleukocytepopulation---structure:LM:round,10-12umindiameterrob-likedorpolymorphousnucleus,2-5lobesfineneutrophilicgranules-pinkcytoplasm组织胚胎学bloodandhemopoiesis课件41EM:specificgranule:80%small,0.3-0.4umovoidorirregularinshapecontain:lysozyme,phagocytin(defensin)

azurophilicgranule:20%large,roundorovoid,0.6-0.7umelectrondenselysosome:acidphosphatase,peroxidase,acidichydrolase组织胚胎学bloodandhemopoiesis课件42---function:phagocytosebacterium:specificg.-killBazurophilicg.-digestB---lifespan:inblood6-8hours,inCT2-3days---function:phagocytosebacte43

②

eosinophil:---normalpercentage:0.5-3%oftotalleukocytepopulation---structure:LM:round,10-15umindiameter2lobesnucleusroughbrightredgranules-acidophilicgranules组织胚胎学bloodandhemopoiesis课件44EM:granules:roundorovoidwithcube-likedelectrondensecrystalcontain:-ACPase-histaminase-peroxidase---function:counteracttheinfectionofparasitereduceallergicreaction---lifespan:inblood6-8hour,inCT8-12daysEM:granules:45③

basophil:---normalpercentage:0-1%oftotalleukocytepopulation---structure:LM:less,round10-12umlobe,“S”shapedorirregularnucleusbasophilicg.:containsheparin,histamineandacidophilchemotacticfactorcytoplasm:containleukotriene---function:involveinallergicreaction---lifespan:10-15days③

basophil:46④

monocyte:---normalpercentage:3-8%oftotalleukocytepopulation---structure:LM:largeround,14-20umnucleusappearaskidney,horse-shoeorovoidinshapecytoplasm:gray-blueincolor,containazurophilicg.④

monocyte:47EM:azurophilicgranule:lysosome-containperoxidase,ACPase,non-spacialesteraseandlysozyme---function:activelymobileandchemotaxis---lifespan:inblood1-5days,inCTwhichbecomeintomacrophage-mononuclearphagocyticsystem,MPS组织胚胎学bloodandhemopoiesis课件48

⑤lymphocyte:---normalpercentage:20-30%oftotalleukocytepopulation---structure:LM:round,smallLC6-8um,medium-sizedLC9-12um,largeLC13-20umindiameterroundnucleuswithindentation,chromatinappearsasspot-likedandelectron-densecytoplasm:/basophilic:brightblueincolor/less/azurophilicgranule⑤lymphocyte:49EM:/freeribosome/mito./RER---classification:TC:thymusdependentlymphocyte,75%,involveincellularimmunereactionandregulateimmuneresponseBC:bonemarrowdependentlymphocyte,10-15%,becomeintoplasmacell,involveinhumoralimmuneresponselargegranularcell:KC(killercell)andNKC(naturekillercell),10%---function:involveinimmuneresponse组织胚胎学bloodandhemopoiesis课件503)bloodplatelet:cytoplasmicfragmentofmegakaryocyteinbonemarrow---normalnumber:100-300X109/L---structure:LM:disc-liked,2-4um→irregularinshapeStimulationingroupsGranulomerehyalomere3)bloodplatelet:cytoplasmic51EM:/cellcoat:glycosaminoglycanandglycoprotein/tubularsystems:openingtubulesystem-increasingtheexchangearea,facilitatetheintakingandreleasingdensetubulesystem-SER,collectingCa2+andsynthesizingprostaglandin/granules:specificgranule:mediateelectrondensity,withdensecore,containsclottingfactor,fibrinogen,acidichydrolasedensegranule:electrondense,containsserotonin(5-hydroxytryptamine),ADP,ATP,Ca2+andadrenalinEM:52组织胚胎学bloodandhemopoiesis课件53---function:involveinclottingandstoppingthebleed:-aggregation-releasecomponentsofgranule,makethrombinogenbecomeintothrombin,thenlatermakefibrinogenbecomeintofibrinprotectingendotheliumandtakepartinrepairingofendothelium---function:543.

Bonemarrowandhemopoiesis1)Placesforhemopoiesisandstructureofbonemarrow①Placesforhemopoiesis:yalksac(3W)→liver(6W)→spleen(4M)→bonemarrow3.

Bonemarrowandhe55②Structureofbonemarrow:redbonemarrow---hemopoietictissue:reticularT.hemopoieticcell,macrophage,fibroblast,lipid-ladencellandmesenchymalcell---sinusoid:cap.组织胚胎学bloodandhemopoiesis课件56*hemopoieticinductivemicroenvironment(HIM):

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