益髓生血颗粒对辐射损伤小鼠骨髓细胞c-ebpα、gm―csf、gm―csfrα、mafbmrna表达的影响

-精选财经经济类资料- -最新财经经济资料-感谢阅读- 1 益髓生血颗粒对辐射损伤小鼠骨髓 细胞 C/EBP、GMCSF、GMCSFR 、MAFBmRNA 表达的影响 kg d）灌胃。采用 60Co- 射线全身 一次性照射造模。造模第 2 天，G-CSF 组按 30 g/（ kg d）皮下注射 G-CSF 注射液，益髓生血颗粒高、中、低剂量 组给药方法同预防给药，其余组均给予 等量蒸馏水，连续 14 d。进行小鼠骨髓 细胞计数；采用实时荧光定量 PCR（Q- PCR）检测骨髓细胞 C/EBP、GM- CSF、GM-CSFR 、MAFB mRNA 表达。 结果 益髓生血颗粒能明显提高辐射损 伤小鼠骨髓细胞计数（均 P 中国论文 -精选财经经济类资料- -最新财经经济资料-感谢阅读- 2 网 /6/view-12955606.htm 关键词 益髓生血颗粒；辐射损 伤；CCAAT 增强子结合蛋白 ；粒-单 系祖细胞集落刺激因子；粒-单系祖细 胞集落刺激因子受体 ；MAFB；基因 表达 中图分类号 R818.02 文献标识 码 A 文章编号 1673-7210（2017） 09（a）-0017-06 Abstract Objective To study the influence of Yisui Shengxue Granules on CCAAT/enhancer binding protein （C/EBP） ， granulocyte-macrophage colon stimulating factor （GM-CSF） ， granulocyte-macrophage colon stimulating factor receptor alpha （GM-CSFR） and transcription factor MAFB mRNA expression in bone marrow cells of mice irradiated by 60Co- rays， and to explore its mechanism of promoting bone marrow hematopoiesis. Methods Seventy- eight male Kunming mice were randomly -精选财经经济类资料- -最新财经经济资料-感谢阅读- 3 divided into normal control group， model group， granulocyte-colony stimulating factor （G-CSF） group， low-dose， middle-dose and high-dose Yisui Shengxue Granules group. Two days before modeling， mice in low-dose， middle-dose and high-dose Yisui Shengxue Granules group received 12， 6， 3 g/（ kg d） Yisui Shengxue Granules ig respectively. On modeling day except normal control group， mice were established by 60Co- ray in other five groups. The 2nd day after modeling， mice in G-CSF group received 30 g/（kg d） G-CSF by injection， mice in normal control group and model group received the same volume distilled water， mice in low-dose， middle-dose and high-dose Yisui Shengxue Granules group received the same way as before modeling， the course lasted 14 days. Bone marrow cells were counted. The -精选财经经济类资料- -最新财经经济资料-感谢阅读- 4 mRNA expression of C/EBP， GM- CSF， GM-CSFR and MAFB in BM cells was analyzed by real-time fluorescent quantitative polymerase chain reaction. Results The data showed that， Yisui Shengxue Granules could increase the count of bone marrow cells of mice irradiated by 60Co- rays （all P 0.05） ； 益髓生血颗粒低剂量组小鼠骨髓细胞 GM-CSF mRNA 表达明显高于正常组及 模型组（均 P 0.05） ，而对 GM- CSF、GM-CSFR 、MAFB mRNA 表达 具有上调作用（均 P 0.05） 。 综上所述，本研究