JIS-K-3602-1990-ENG.pdf

jajis udc 628.322.3:541 .i 35.5:53.082.9 japanese industrial standard apparatus for the estimation of biochemical oxygen demand (bod,) with microbial sensor jis k 3 6 0 2 - 1 9 9 0 translated and published by japanese standards association printed in japan copyright japanese standards association provided by ihs under license with jsalicensee=ihs employees/1111111001, user=wing, bernie not for resale, 03/12/2007 02:52:31 mdtno reproduction or networking permitted without license from ihs -,-,- i j i s k83602 70 4733608 0086324 7 = in the event of any doubt arising, the original standard in japanese is to be final authority. copyright japanese standards association provided by ihs under license with jsalicensee=ihs employees/1111111001, user=wing, bernie not for resale, 03/12/2007 02:52:31 mdtno reproduction or networking permitted without license from ihs -,-,- udc 628.312.3:541.135.5:53.082.9 japanese industrial standard j i s apparatus for the estimation of biochemical oxygen demand (bods) with microbial sensor k 3602-1990 1. scope this japanese industrial standard specifies the apparatus for estimation (hereafter referred to as the “apparatus for estimation“) of the value of biochemical oxygen demand (hereafter referred to as the “bods“) in the waste water of fermentation waste water, sewage, etc. by utilizing the microbial sensor of combining an immobilized microbial membrane with an oxygen electrode. , remarks: applicable standards in this standard are shown in attached table 1. 2. definitions for the main terms used in this standard the definitions in jis k 0211, jis k 3600, and jis z 8103 apply, and the rest of the terms shall be as follows. ( 1 ) immobilized microbial membrane be the yeast trichosporon cutaneum, ifo-10466 sealed in a membrane under a state wherein its physiological function is kept. an immobilized microbial membrane shall (2) oxygen electrode measurement of dissolved oxygen specified in 7.3 (1) (electrode) of jis k 0803. an oxygen electrode shall be an electrode for (3) microbial sensor a microbial sensor shall be constructed by combining an immobilized microbial membrane with an electrode which detects the variation of the substance concentration to be generated when its microbe has decomposed and ingested organic substance, for example, the decrease of oxygen concentration following the increase of the respiration activity of microbe. 3. structure of microbial sensor and measuring system 3.1 immobilized microbial membrane an immobilized microbial membrane shall be of the type which covers the surface of an oxygen electrode. 3.2 oxygen electrode either of the following electrodes. the oxygen electrode shall be in accordance with ( 1) diaphragm polarograph type oxygen electrode (2) diaphragm galvanic cell type oxygen electrode 3.3 measuring method of bods value the measuring method of a bods value shall be in accordance with -either of the following systems. (1) batch system (2) flow system copyright japanese standards association provided by ihs under license with jsalicensee=ihs employees/1111111001, user=wing, bernie not for resale, 03/12/2007 02:52:31 mdtno reproduction or networking permitted without license from ihs -,-,- j i s k83602 90 4933b08 ooab32b o m 2 k 3602-1990 3.4 calculation system of bods value the calculation system of bods value shall be in accordance with either of the following systems. (1) a system of calculation from the maximum variation amount of oxygen electrode current. (2) a system of calculation from the variation amount after a specific time of oxygen electrode current. 4 . rated voltage and rated frequency the rated voltage of an apparatus for estimation shall be single phase ac 100 v and the rated frequency thereof shall be 50 hz, 60 hz, or common 50 hz/60 hz. 5, performance an apparatus for estimation shall conform to the following performances. (1) repeatability the deviation shall be f 10 % of the mean value. when a test is performed by the testing method of 7.3 ( i ) , (2) response time (2), the response time shall be within 20 min. when a test is performed by the testing method of 7.3 (3) stability to voltage fluctuation method of 7.3 (3), the variation of its indication value shall be 4 3 %. when a test is performed by the testing (4) insulation resistance 7.3 (4), its value shall be 5 ms1 or over. when a test is performed by the testing method of (5) withstand voltage (5), any abnormality shall not be generated, when a test is performed by the testing method of 7.3 6. construction 6.1 general in construction the construction of an apparatus for estimation shall conform to the following respective items. the shape shall be regular, and the assembly and finish of each part shall be good and strong. there is no fear for risks under an ordinary operation state, and the apparatus for estimation shall operate safely and smoothly. each part shall be so constructed as not to cause mechanical and electrical troubles and not to generate risks. the apparatus for estimation shall be so constructed not to cause the disturb of the operation of the apparatus for estimation due to water leak, water splash, dew condensation, etc. the apparatus for estimation shall be so constructed as to operate easily in maintenance and inspection and to be free from dangers. copyright japanese standards association provided by ihs under license with jsalicensee=ihs employees/1111111001, user=wing, bernie not for resale, 03/12/2007 02:52:31 mdtno reproduction or networking permitted without license from ihs -,-,- 3 k 3602-1990 6.2 structure part, a supply part of buffer solution or the like, a discharge part, and an indication recording part. fig. 1. the apparatus for estimation shall be composed of a detection structure of apparatus for estimation (an example) i ! detection part a : microbial sensor 8 : measuring ce11 - : flow of sample - : signal 6.3 detection part a detection part shall keep a microbial sensor and sample solution under a state of being in contact with each other, and shall supply a signal to be generated to an indication part stably, which is composed of the microbial sensor, a measuring cell, etc. (1) microbial sensor a microbial sensor shall be composed of an immobilized microbial membrane (the immobilized membrane of trichosporon cutaneum ifo-10466) with oxygen electrode, and the immobilized microbial membrane shall be so constructed as to be easily interchangeable. (2 6.4 measuring cell a measuring cell shall keep a microbial. sensor and sample solution under a state of being in contact with each other, and shall be.so constructed as to keep the sample solution at a suitable temperature and oxygen concentration, and as not to generate any influence on the flow of the sample solution. supply part of buffer solution or the like a supply part of buffer solution or the like shall be able to send buffer solution or the like to a detection part. 6.5 discharge part a discharge part shall be able to discharge and reserve the solution after measurement from the detection part. copyright japanese standards association provided by ihs under license with jsalicensee=ihs employees/1111111001, user=wing, bernie not for resale, 03/12/2007 02:52:31 mdtno reproduction or networking permitted without license from ihs -,-,- jis k*3602 90 w 4933b08 0086328 4 4 k 3602-1990 6.6 indication recording part an indication recording part shall be able to indicate or print a bods value or its equivalent value, otherwise an index based on the output current of microbial sensor. 6.7 attachment the following devices may be annexed as attachment if the occasion arises. (1) sample solution feeder a sample solution feeder sends some preliminarily prepared samples to a detection part following the determined order to be measured, it should preferably have a function capable of calibrating a microbial sensor by sending the bods calibrating solution of 7.1 (5) on occasion. (2) output current recorder record the output current of a microbial sensor. an output current recorder shall be able to 7. tests 7.1 reagents reagents to be used for tests shall be as follows. water water for analysis specified in jis k 0050. glucose l-glutamic acid glucose specified in jis k 8824. l-glutamic acid specified in jis k 9047. bods calibration stock solution bods calibration stock solution is prepared by dissolving 150 mg of glucose and 150 mg of l-glutamic acid, with water in a 1000 ml volumetric flask, to the mark line. value of this calibration stock solution in accordance with the measuring method for biochemical oxygen demand (bod) specified in 21. of jis k 0102. shall be confirmed to be 220 f 10 mg/. this calibration stock solution shall be used as soon as possible after preparation. the bod bods calibration solution weighing out the bods calibration stock solution so as to have the bod value described in an instruction manual into a volumetric flask and adding water to the mark line. bods calibration solution is prepared by further, the solution which is prepared in accordance with this method and is supplied by the manufacturer may be taken as the bods calibra tion solution. buffer solution buffer solution described in an instruction manual. 7.2 testing conditions the testing conditions shall be as follows. (1) measuring cell temperature a measuring cell temperature described in an instruction manual. (2) (3) relative humidity 65 f 20 % (4) power supply voltage rated voltage ambient temperature 20c or over up to and including 3ooc. copyright japanese standards association provided by ihs under license with jsalicensee=ihs employees/1111111001, user=wing, bernie not for resale, 03/12/2007 02:52:31 mdtno reproduction or networking permitted without license from ihs -,-,- j i s k*3602 90 = 4933608 0086329 b _ _ _ _ _ _ 5 k 3602-1990 (5) preliminary running preliminary running shall be performed by the method described in an instruction manual. (6) calibration after preliminary running, calibration shall be performed by the method described in an instruction manual by using bods calibration solution. 7.3 testing methods the testing methods shall be as follows. o (3) (4) (5) repeatability in an instruction manual, and obtain the mean value of the obtained data and the deviation of the mean value from each measured value. feed five times the bods calibration solution described response time reached a specific value under a state wherein buffer solution is sent into a measuring cell, feed bods calibration solution, record an output current, and obtain the time (response time) until the difference of output current before and after feeding the solution reaches 90 % of an final value. measure by a batch system for an apparatus for estimation capable of operating by the batch system, and make the feeding amount of bods calibration solution to the specified amount. after an output current of a microbial sensor has further, for an apparatus for estimation capable of operating only by a flow system, measure by the flow system, and continue to send the specified amount of bods calibration solution at a constant flow rate while measuring. stability to voltage fluctuation rated voltage, and send bods calibration solution by the method of (2). keep the power supply voltage at the . . after the indicating value has reached the constant“va1ue (the final value), vary the power supply voltage by f 10 % of the rated voltage. take the ratio in percentage of the variation amount of the indicating value following the voltage variation and the indicating value when kept at the rated voltage as the variation of the indicating value. insulation resistance under a state wherein the electric circuit of an apparatus for estimation is closed, measure the insulation resistance between power supply terminals connected together and casing (earthing terminal) with a dc 500 v megger specified in jis c 1302. withstand voltage between power supply terminals connected together and casing for 1 min under a state wherein the electric circuit of an apparatus for estimation is closed, and confirm non-presence of abnormality. impress 1000 v ac voltage of rated frequency 8. markine: the apparatus for estimation shall be marked by an indelible method with the following information. (1) name and the type name or type of an apparatus for estimation determined by manufacturer. copyright japanese standards association provided by ihs under license with jsalicensee=ihs employees/1111111001, user=wing, bernie not for resale, 03/12/2007 02:52:31 mdtno reproduction or networking permitted without license from ihs -,-,- j i s k83b02 90 = 4933b08 008b330 2 m 6 k 3602-1990 (2) classification and capacity of power supply (3) class of transmission output (if necessary) (4) manufacturers name (or its registered trademark) (5) year and month of manufacture (or its abbreviation) or manufacturing number. 9. instruction manual the following items shall be described in an instruction manual. (1) items concerning use method selection of setting position and use ambient temperature range (ambient temperature range guaranteed by manufacturer) outline of the construction, wiring, and tubing of an apparatus for estimation. the preparation and reservation method of reagents, the reservation method of immobilized microbial membrane (when all of reagents or the like are supplied by manufacturers; the preparation method may be not described), and sanitary consideration concerning treating an immobilized micobial membrane, etc. items concerning: measurement activation of an immobilized microbial membrane preparation of measurement method and frequency of calibration measuring method treatment when measurement is stopped items concerning measurement such as the range wherein the linearity of an apparatus for estimation is effected and the considerable points on interfering substance, etc. (2) items concerning maintenance (a) standard frequency of exchange of immobilized microbial membrane or microbial sensor. (b) exchange of immobilized microbial membrane and assembly method of microbial sensor, or exchange method of microbial sensor. (c) cleaning and maintenance of detection part, tubing, etc. (d) countermeasure in trouble copyright japanese standards association provided by ihs under license with jsalicensee=ihs employees/1111111001, user=wing, bernie not for resale, 03/12/2007 02:52:31 mdtno reproduction or networking permitted without license from ihs -,-,- j i s k*3b