乳腺癌中RUNX3、cyclinD1和p27kip1蛋白的表达及其意义.doc

乳腺癌中RUNX3、cyclin D1和p27kip1蛋白的表达及其意义【摘要】 目的 探讨乳腺癌组织中RUNX3、cyclin D1和p27kip1基因的表达及其临床意义。方法 采用免疫组织化学SP法检测88例乳腺癌、40例乳腺纤维腺瘤、40例乳腺增生病中RUNX3、cyclin D1和p27kip1的表达。结果 （1）RUNX3和p27kip1在乳腺癌中的阳性表达率分别为35.23%和51.14%，明显低于在乳腺纤维腺瘤(85%和82.5%)及乳腺增生病(87.5%和85%)中的表达率（P<0.05）。乳腺癌中cyclin D1的表达率（63.63%）明显高于良性病变中表达。（2）RUNX3 和p27kip1蛋白表达与乳腺癌的临床分期、淋巴结转移呈负相关，p27kip1蛋白表达与病理分级呈负相关。cyclin D1蛋白表达与病理分级呈正相关。（3）乳腺癌中RUNX3与p27kip1蛋白的表达呈正相关性（P<0.05），与cyclin D1的表达呈负相关性（P>0.05）。结论 RUNX3、cyclin D1和p27kip1在乳腺癌的发生发展中起重要作用。检测乳腺癌组织中RUNX3、cyclin D1和p27kip1基因的表达对于评价患者的预后有一定价值。 【关键词】 乳腺肿瘤 Expressions of RUNX3, cyclin D1 and p27kip1 in Breast Cancer and Their Significance Abstract： Purpose To investigate the expressions of RUNX3, cyclin D1 and p27kip1 in breast cancer and their significance. Methods Expressions of RUNX3, cyclin D1 and p27kip1 in 88 cases with breast cancer, 40 cases with breast fibroadenoma and 40 cases with mazoplasia were detected by immunohistochemical SP method. Results (1)The positive expression of RUNX3 and p27kip1 in breast cancer was 35.23% and 51.14% respectively. They were significantly lower than those in breast fibroadenoma (85% and 82.5%) and mazoplasia (87.5% and 85%)(P<0.05).The positive expression of cyclin D1 in breast cancer (63.63%) was significantly higher than that in the benign tissues (P<0.05). (2)The positive expression of RUNX3 and p27kip1 in breast cancer was negatively correlated to the clinical staging or node metastasis（P<0.05）. The expression of p27kip1 was negatively correlated to the histologic grade, but cyclin D1 expression showed positive correlation with histologic grade（P<0.05）. (3) The expression of RUNX3 was positively correlated with the expression of p27kip1, and negatively correlated with cyclin D1. Conclusion RUNX3, cyclin D1 and p27kip1 may play an important role in the carcinogenesis and progression of breast cancer. The detection of RUNX3, cyclin D1 and p27kip1 protein expression has a definite value in evaluating prognosis for breast cancer. Key words: breast neoplasms; RUNX3; cyclin D1; p27kip1; immunohistochemistry RUNX3基因是最近研究发现的一个抑癌基因，定位于人类染色体1p36.1区域1，参与许多肿瘤的发生过程，其在人乳腺癌中的表达及其与cyclin D1和p27kip1表达的相关性少见报道。我们应用免疫组化的方法检测RUNX3蛋白在乳腺癌中的表达情况，分析其与临床病理参数的关系，以及与抑癌基因p27kip1和癌基因cyclin D1蛋白表达的相关性，分析RUNX3基因在乳腺癌中的作用及其与其他肿瘤相关基因之间的关系。 1 材料与方法 1.1 标本来源 收集哈尔滨医科大学附属第二医院病理科19971998年乳腺手术标本168例，其中乳腺癌88例、乳腺纤维腺瘤40例、乳腺增生病40例。乳腺癌患者年龄2783岁，中位年龄52岁。原位癌18例，浸润性癌70例。其中导管癌57例(导管原位癌11例，浸润性导管癌46例)，小叶癌31例（小叶原位癌7例，浸润性小叶癌24例）。临床分期：期52例，期36例。 1.2 免疫组化方法 采用免疫组织化学SP法，即链霉素抗生物素蛋白过氧化物酶法检测乳腺癌组织中RUNX3蛋白的表达。实验步骤按说明书进行。浓缩型RUNX3兔抗人多克隆抗体购自Active Motif公司，鼠抗人cyclin D1、p27kip1单克隆抗体、羊抗兔二抗、羊抗鼠二抗及DAB试剂均购自福州迈新生物技术公司。采用PBS液代替一抗作为阴性对照，采用已知的阳性片作为阳性对照。 1.3 结果判定标准 RUNX3蛋白在乳腺癌、乳腺纤维