人骨肉瘤中5个新基因片段在不同骨肿瘤中的差异表达.doc

5 :;PCR; :.5RT-PCR376819.375:ZOL0392.6%1C8289.4%1C7473.9%2G1266.5%2A2153.7%;23:ZOL036.5%1C8214.7%1C748.3%2G1211.9%2A2112.2%;29:ZOL038.3%1C8215.6%1C7412.5%2G1213.8%2A215.4%;16:ZOL039.4%1C8214.3%1C747.5%2G126.8%2A216.2%;19:ZOL0301C826.4%1C7402G124.8%2A213.9%;5P<0.01;P<0.01.ZOL031C821C742G122A2155. Keywords:osteosarcoma;differentialexpressedgene;RT-PCR;geneexpression Abstract:AIMToscreenseveralgenesdifferentiallyex-pressedinhumanosteosarcomawithhigherexaminationratesanddiscussitsclinicalsignificance.METHODSByusingZOL031C821C742G12and2A21asresearchtargets37specimensofhumanosteosarcomaand68specimensofothermusculoskeletaltumorsaswellas19specimenswerescreenedbyquantitativeRT-PCR.RESULTSIn37speci-mensofosteosarcomapercentagesofthe5fragmentswithhigherexaminationratewere92.6%ZOL0389.4%1C8273.9%1C7466.5%2G12and53.7%2A21sequentially.In23specimensofosteochondromapercent-agesofthe5fragmentswithhigherexaminationratewere6.5%ZOL0314.7%1C828.3%1C7411.9%2G12and12.2%2A21respectively.In29specimensofgiantcelltumorofbonethepercentagesofthe5fragmentsinturnwere8.3%ZOL0315.6%1C8212.5%1C7413.8%2G12and5.4%2A21.In16specimensofchondrosarcomathepercentagesinsuccessionwere9.4%ZOL0314.3%1C827.5%1C746.8%2G12and6.2%2A21.Theexpressionratesofthe5fragmentsbetweenosteosarcomaandothermusculoskeletaltumorsweresignificantlydifferentP<0.01.Andin19specimensofnormalbonetissuetheexpressionratesofthe5frag-mentswere0ZOL036.4%1C8201C744.8%2G12and3.9%2A21.Theexpressionratesofthe5fragmentsbetweenosteosarcomaandnormalbonetissueweresignificantlydifferentP<0.01.CONCLUSIONThe5fragmentsofdifferentiallyexpressedgenesisolatedfromhu-manosteosarcomatissuemaybecorrelatedwithoccurrenceandprogressofosteosarcoma. 0 .ZOL031C821C742G122A21PCRDD-PCRRT-PCR 1 1.1:37;68232916;19./HE.:PCR.:MMLVPromegaPE5700PCRPE. 1.2.PromegaPolyATtractRSystem1000mRNA0.1g・L-1mRNA:oligodT18RNase10.4L5Buffer4.0LdNTP250mol・L-11.6LmRNA0.1g・L-11.0LoligodT182.0L;:655min3760min755minMMLV200mU・L-13710min.2L:10PCRBuffer3L2.5mmol・L-1dNTPs3L1L10pmol1L10pmolddH2O10L2.5UTaq;PCR:9436s551min7235s35727min.PCR-actin.PCR10g・L-120minGQS-96002/. 2 2.1375:ZOL0392.6%1C8289.4%1C7473.9%2G1266.5%2A2153.7%.5PCRFig1. 2.223:ZOL036.5%1C8214.7%1C748.3%2G1211.9%2A2112.2%.29:ZOL038.3%1C8215.6%1C7412.5%2G1213.8%2A215.4%.16:ZOL039.4%1C8214.3%1C747.5%2G126.8%2A216.2%.5. 15 2.3195:ZOL0301C826.4%1C7402G124.8%2A213.9%.5. /5P<0.01;P<0.01.5. 3 2;.3-5.123112GenBank.11PCR5;5P<0.015. : 1CuiDXYanXJZhangLZhaoJRJiangMGuoYHZhangLXBaiXPSuCZ.Screeninganditsclinicalsignificanceof6fragmentsofhighlyexpressinggenesingastriccancerandpre-cancerousmucosaJ.ShijieHuarenXiaohuaZazhiWorldChinJDigestol1999;79:770-772. 2LiLZWangYJLiYLiZY.Clinicalsignificanceofexpres-sionoftumorsuppressorgenesandproliferativeactivityinos-teosarcomaJ.JClinOrthop1999;23:164-166. 3CuiDXYanXJFanQYSuCZJiCH.Studiesonrelation-shipbetweenmusculoskeletalneoplasmandoncogenemdm2metastastissuppressorgenenm23-H1J.Di-siJunyiDaxueXuebaoJFourthMilMedUniv1997;181:20-23. 4LiDCuiDXSuHCYanXJFanQY.Alterationofmdm2andp53inmusculoskeletaltumorsanditsclinicalsignificanceJ.Di-siJunyiDaxueXuebaoJ