喉癌组织中基质金属蛋白酶MMP┐2及其抑制剂TIMP┐2的表达.doc

MMP2TIMP2 :; :MMP-2TIMP-2.LSAB645MMP-2TIMP-2.MMP-274%39%TIMP-252%24%27.1800P<0.055.0420P<0.05.MMP-2TIMP-2.MMP-2TIMP-2. Keywords:laryngealneoplasms;matrixmetalloproteinase;immunohistochemical;lymphaticmetastasis Abstract:AIMTostudytheexpressionsofmatrixmetallo-proteinase-2andtissueinhibitorofmetalloproteinase-2inhu-manlaryngealsquamouscancerandtherelationshipbetweentheexpressionsandclinicalfeatures.METHODSMMP-2andTIMP-2wereexaminedwithimmunohistochemicalSABmethodintheparaffinembeddedspecimensfrom64caseswithlaryngealsquamouscellcarcinomaLSCCand5normalswhoservedasthecontol.RESULTSInthegroupofcervicallymphnodeinvolvementandnon-cervicallymphn-odeinvolvementtheirpositiveratesofMMP-2were74%and39%andtheirpositiveratesofTIMP-2were52%and24%MMP-2s2=7.1800P<0.05;TIMP-2s2=5.0420P<0.05whichshowedasignificantcorrelationbetweenthem.MMP-2andTIMP-2hadnorelationshipwiththesituationandmalignantdegree.BothMMP-2andTIMP-2wereexpressedmorestronglyincarcinomawithcervicallymphnodeinvolvementgroupthaninnon-cervicallymphn-odeinvolvementgroupbutthehighTIMP-2expressioncouldnotcontroltheactivityofMMP-2.Nonbalancebe-tweenMMP-2andTIMP-2borenorelationshiptohumanla-ryngealsquamouscellcarcinomainvasionandmetastasis.CONCLUSIONThedetectionofMMP-2andTIMP-2ex-pressionsmaycontributetodiagnosisandprognosisofLSCC. 0 matrixmetalloproteinasesMMPsextracellmatrixECM;tissueinhibitorofmetalloteinasesTIMPs12.MMPsTIMPs.MMPSTIMPS64MMP-2TIMP-2. 1 1.11997/19996453114175.142921.:I18II33III13.2341.1987T1T243T3T421.540g・L-1. 1.24m4m.30mL・L-1H2O210min.PBS5min3.:MMP-240g・L-13740min;TMP-210min9098.PBS5min3.100mL・L-13710min1100MMP-2mAb1100TIMP-2mAb4PBS5min311003730minPBS5min31:1003730minPBS5min3DAB20min.5<10%>10%. :23. 2 MMP-2TIMP-2.MMP-2TIMP-2Fig1. 1 1MMP-2TIMP-2 3 MMPs4.3.IVIV.MMP-2MMPsAIV.TIMPsMMPsTIMPsTIMP-1TIMP-2MMP-211TIMP-2MMP-2TIMP-1710TIMP-2MMP-2ECMTIMP-2MMP-2. MMP-256.TIMP-2TIMP-2.MMP-2TIMP-2.MMP-274%17/2339%16/41.TIMP-252%12/2324%10/41P<0.05.TIMP-2MMP-2.MMP-2-TIMP-2.MMP-2TIMP-2. : 1XieYMNieQHZhouYXKangWZZhuXHWangYM.TIMP-1and-2relatedantigensexpressioninlivercarcinomatis-suesJ.Di-siJunyiDaxueXuebaoJFouthMilMedUniv2001;2219:1787-1790. 2XieYMNIEQHZhouYXZhuXHWangJPDuanGR.Tissueinhibitorsofmetalloproteinase-1and-2TIMP-1andTIMP-2relatedantigensexpressioninlivertissuesofpatientswithlivercirrhosisJ.Di-siJunyiDaxueXuebaoJFourthMilMedUniv2000;217:790-792. 3LCLLiaoBJFanJP.ThedetectionofHPVinfectionP16expressioninlaryngealsquamouscancerandthestudyoftheirrelationshipwithclinicalcasesJ.ClinExpPathol1999;155:382-384. 4HeSYXinXYWangT.Expressionofmatrixmetallopi-oteinase-2andCD147inovarianepithelialtumorJ.Di-siJun-yiDaxueXuebaoJFourthMilMedUniv2001;225:435-438. 5LiuDCChenBQWangH.Expressionandsignificanceofvas-cularendothelicalgrowthfactorinbladdertransitionalcellcarci-nomaJ.Di-siJunyiDaxueXuebaoJFourthMilMedUniv2000;2111:1346-1348. 6DuPWeiJGLiHPWangYXWuQZHuangJ.Expres-sion