立克次体属汇总.ppt

Rickettsia,徐桂香 王莹 赵云鹏 张爱娟 张璐娴 朱玉,Howard Ricketts,立克次体属 (Rickettsia) 柯克斯体属 立克次体目 东方体属 埃立克体属 巴通体属,Reckettsiales,Common characters,Obligated intracellular parasites; Related to arthropod vector.; Zoonosis.,Morphology & stains,Arranged irregularly; polymorphism Small G- bacilli; Giemsa stain: violet or blue;,Cultural properties,Binary fission Have both DNA and RNA Culture :susceptible animal (guinea pig), yolksac of chick embryo, cell culture.,Resistance,weak, sensitive to antibiotic Survive in low temperature and dry condition Sulfanilamide can stimulate reckettsias proliferation,Antigenic sturcture,Group specific antigen Endotoxin: similarly to endotoxin of bacteria Specy specific antigen membrane protein: Weil-Felix reaction(外斐反应）: Detect anti-rickettsial antibodies in a patients serum by agglutination of the proteus organisms. ( rickettsia posses Ag that cross-react with Ab of the O antigen of certain proteus),Pathogenic mechanism,Pathogenic material,Pathogenic mechanism,Local（ small blood vessels in lymphoid tissue ）,vascular endothelial cells,Fever, headache, rash,(First bacteremia),(Second bacteremia),vessels,Endotoxin 、 Phospholipase A,Bite or feces of arthropod vector,Pathogenic sites,Pathogenicity,Pathogenic materials Endotoxin: similarly to endotoxin of bacteria Phospholipase A: lysis the cell membrane and lysosome membrane of the host cell, causing hemolysis Pathogenic mechanism: invade the epithelial cells of small blood vessule,Pathogenic mechanism,Pathogenic material,Pathogenic mechanism,Local（ small blood vessels in lymphoid tissue ）,vascular endothelial cells,Fever, headache, rash,(First bacteremia),(Second bacteremia),vessels,Endotoxin 、 Phospholipase A,Bite or feces of arthropod vector,Pathogenic sites,Infective route,Source:Rickettsiae normally enter the body (mammalian reservoir) through the bite or feces of an infected arthropod vector.-arthropod vector: mite, tifck, louse, flea Pathogenic mechanism: invade the epithelial cells of small blood vessule,Pathogenic mechanism,Pathogenic material,Pathogenic mechanism,Local（ small blood vessels in lymphoid tissue ）,vascular endothelial cells,Fever, headache, rash,(First bacteremia),(Second bacteremia),vessels,Endotoxin 、 Phospholipase A,Bite or feces of arthropod vector,Pathogenic sites,主要病原性立克次体,种类 传染源 媒介昆虫 主要临床表现 免疫力 普氏立克次体 人 人体虱 发热、头痛、皮疹 持久 （流行性斑疹伤寒） 神经系统、心血管系统损伤 斑疹伤寒立克次体 鼠 鼠蚤 发热、头痛、皮疹 持久 （地方性斑疹伤寒） 鼠虱 恙虫病东方体 鼠 恙螨 局部黑色焦痂 持久 （恙虫热） 发热、头痛、皮疹 内脏损害 贝纳柯克斯体 家畜 蜱 发热、头痛 一定程度 （动物间） 心内膜炎,Epidemic typhus (R.prowazekii) 普氏立克次体,Louse-borne; substandard living condition, poor sanitaion; overwhelming bacterimia,Human,Human louse,Human,Human louse,Epidemic typhus (R.prowazekii) 普氏立克次体,Fever (temperature rises to 40C; Treatment: sanitation, eradication of human lice; vaccine, tetracycline,主要病原性立克次体,种类 传染源 媒介昆虫 主要临床表现 免疫力 普氏立克次体 人 人体虱 发热、头痛、皮疹 持久 （流行性斑疹伤寒） 神经系统、心血管系统损伤 斑疹伤寒立克次体 鼠 鼠蚤 发热、头痛、皮疹 持久 （地方性斑疹伤寒） 鼠虱 恙虫病东方体 鼠 恙螨 局部黑色焦痂 持久 （恙虫热） 发热、头痛、皮疹 内脏损害 贝纳柯克斯体 家畜 蜱 发热、头痛 一定程度 （动物间） 心内膜炎,Endemic typhus (R. mooseri) 斑疹伤寒立克次体,Transmitted way:,rat,rat flea,rat louse,rat flea,rat,human louse,human,human,human louse,fever;headack:maculopapular rash,主要病原性立克次体,种类 传染源 媒介昆虫 主要临床表现 免疫力 普氏立克次体 人 人体虱 发热、头痛、皮疹 持久 （流行性斑疹伤寒） 神经系统、心血管系统损伤 斑疹伤寒立克次体 鼠 鼠蚤 发热、头痛、皮疹 持久 （地方性斑疹伤寒） 鼠虱 恙虫病东方体 鼠 恙螨 局部黑色焦痂 持久 （恙虫热） 发热、头痛、皮疹 内脏损害 贝纳柯克斯体 家畜 蜱 发热、头痛 一定程度 （动物间） 心内膜炎,Immunity,Mainly depends on CMI Strong immunity after recovery,Microbiological examination,Specimen : blood Isolation and culture: guinea pig Serological test: Weil-Felix reaction,Principle of prevention and treatment,Control a