DNA固定在纳米颗粒表面的方法.ppt

磷酸化的DNA Biotin-DNA 炔基修饰的DNA 巯基修饰的DNA 氨基修饰的DNA,金银纳米颗粒 碳纳米管、石墨烯 二氧化硅纳米颗粒 量子点 上转换纳米颗粒,磷酸化的DNA与TiO2 -堆积，如单链DNA与石墨烯，碳纳米管 静电吸附,1、Biotin-DNA,2、炔基修饰的DNA及其他生物分子,3、NH2-DNA,氨基 羧基,氨基 羧基 氨基 氨基,1）EDC和NHS,For best results in two-step reactions, perform the first reaction in MES buffer (or other non-amine, non-carboxylate buffer) at pH 5-6, then raise the pH to 7.2-7.5 with phosphate buffer (or other non-amine buffer) immediately before reaction to the amine-containing molecule,氨基 羧基,DCC（N,N-Dicyclohexylcarbodiimide）,氨基-氨基,1）BS(PEG)n PEG Crosslinkers - Amine Reactive,2）TSAT (Tris-succinimidyl aminotriacetate),Water,Water,NHS esters:pH 7-9,3）DSS (disuccinimidyl suberate),4）BSOCOES (bis2-(succinimidyloxycarbonyloxy)ethylsulfone),5）DSG (disuccinimidyl glutarate),6）DTSSP (3,3-Dithiobissulfosuccinimidylpropionate),Water,Water,Water,DMSO or DMF,8）BS (Bissulfosuccinimidyl suberate),9）EGS ethylene glycolbis(succinimidylsuccinate),10）Sulfo-EGS ethylene glycolbis(sulfosuccinimidylsuccinate),7）DST (disuccinimidyl tartrate),Water,Water,DMSO or DMF,DMSO or DMF,4、巯基化的DNA,1 Au-S Ag-S 2 巯基 氨基 3 巯基 羧基 4 巯基 羟基 5 巯基 巯基,巯基-氨基,1）AMAS (N-maleimidoacetoxy succinimide ester),NHS esters:pH 7-9；Maleimides:pH 6.5-7.5；Usually 7.2-7.5。Two steps reaction,DMSO or DMF,2）BMPS (N-maleimidopropyloxy succinimide ester),DMSO or DMF,3）EMCS (N-maleimidocaproyloxysuccinimide ester),DMSO or DMF,4）Sulfo-EMCS (N-maleimidocaproyloxysulfosuccinimide ester),Water,6）Sulfo-GMBS (N-maleimidobutyryloxysulfosuccinimide ester),5）GMBS (N-maleimidobutyryloxysuccinimide ester),DMSO or DMF,Water,7）BMPA (N-maleimidopropionic acid),EDC，0.1mol MES pH4.7 Maleimides react with sulfhydryls (SH) at pH 6.5-7.5,8）LC-SMCC (Succinimidyl-4-N-maleimidomethylcyclohexane-1-carboxy-6-amidocaproate),DMSO or DMF,10）Sulfo-SMPB (sulfosuccinimidyl 4-p-maleimidophenylbutyrate),9）SMPB (succinimidyl 4-p-maleimidophenylbutyrate),DMSO or DMF,Water,11）Sulfo-LC-SPDP, LC-SPDP, SPDP,巯基-羧基,巯基-羟基,异氰酸脂在水溶液中很容易水解，所以最好用有机溶剂如DMSO、DMF等。如在水相中，则要使用pH8.5的碱性缓冲溶液，避免使用含羟基的缓冲液如tris缓冲液，且要注意含羟基的分子不能有氨基和巯基，因为pH7.5顺丁烯二酰亚胺失去对巯基的特异性，会同时与氨基和巯