psPAX2慢病毒载体使用说明.pdf

psPAX2 psPAX2 载体基本信息载体基本信息： 载体名称载体名称: psPAX2 质粒类型质粒类型: Lentiviral Packaging Plasmids(慢病毒包装质粒）慢病毒包装质粒） 高拷贝高拷贝/低拷贝低拷贝: 高拷贝高拷贝 启动子启动子: - 克隆方法克隆方法: 多克隆位点，限制性内切酶多克隆位点，限制性内切酶 载体大小载体大小: 10703bp 5 测序引物及序列测序引物及序列: - 3 测序引物及序列测序引物及序列: - 载体标签载体标签: - 载体抗性载体抗性: 氨苄氨苄 筛选标记筛选标记: - 备注备注: 慢病毒包装质粒慢病毒包装质粒 稳定性稳定性: 稳定稳定 组成型组成型: 非组成型非组成型 病毒病毒/非病毒非病毒: 病毒病毒 psPAX2 载体质粒图谱和多克隆位点信息载体质粒图谱和多克隆位点信息： 编号编号 载体名称载体名称 北京华越洋北京华越洋 VECT231272 psPAX2 psPAX2 载体序列载体序列： ORIGIN 1 GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA 61 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC 121 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG 181 GGACTTTCCA TTGACGTCAA TGGGTGGACT ATTTACGGTA AACTGCCCAC TTGGCAGTAC 241 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG 301 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG 361 TATTAGTCAT CGCTATTACC ATGGGTCGAG GTGAGCCCCA CGTTCTGCTT CACTCTCCCC 421 ATCTCCCCCC CCTCCCCACC CCCAATTTTG TATTTATTTA TTTTTTAATT ATTTTGTGCA 481 GCGATGGGGG CGGGGGGGGG GGGGGCGCGC GCCAGGCGGG GCGGGGCGGG GCGAGGGGCG 541 GGGCGGGGCG AGGCGGAGAG GTGCGGCGGC AGCCAATCAG AGCGGCGCGC TCCGAAAGTT 601 TCCTTTTATG GCGAGGCGGC GGCGGCGGCG GCCCTATAAA AAGCGAAGCG CGCGGCGGGC 661 GGGAGTCGCT GCGTTGCCTT CGCCCCGTGC CCCGCTCCGC GCCGCCTCGC GCCGCCCGCC 721 CCGGCTCTGA CTGACCGCGT TACTCCCACA GGTGAGCGGG CGGGACGGCC CTTCTCCTCC 781 GGGCTGTAAT TAGCGCTTGG TTTAATGACG GCTCGTTTCT TTTCTGTGGC TGCGTGAAAG 841 CCTTAAAGGG CTCCGGGAGG GCCCTTTGTG CGGGGGGGAG CGGCTCGGGG GGTGCGTGCG 901 TGTGTGTGTG CGTGGGGAGC GCCGCGTGCG GCCCGCGCTG CCCGGCGGCT GTGAGCGCTG 961 CGGGCGCGGC GCGGGGCTTT GTGCGCTCCG CGTGTGCGCG AGGGGAGCGC GGCCGGGGGC 1021 GGTGCCCCGC GGTGCGGGGG GGCTGCGAGG GGAACAAAGG CTGCGTGCGG GGTGTGTGCG 1081 TGGGGGGGTG AGCAGGGGGT GTGGGCGCGG CGGTCGGGCT GTAACCCCCC CCTGCACCCC 1141 CCTCCCCGAG TTGCTGAGCA CGGCCCGGCT TCGGGTGCGG GGCTCCGTGC GGGGCGTGGC 1201 GCGGGGCTCG CCGTGCCGGG CGGGGGGTGG CGGCAGGTGG GGGTGCCGGG CGGGGCGGGG 1261 CCGCCTCGGG CCGGGGAGGG CTCGGGGGAG GGGCGCGGCG GCCCCGGAGC GCCGGCGGCT 1321 GTCGAGGCGC GGCGAGCCGC AGCCATTGCC TTTTATGGTA ATCGTGCGAG AGGGCGCAGG 1381 GACTTCCTTT GTCCCAAATC TGGCGGAGCC GAAATCTGGG AGGCGCCGCC GCACCCCCTC 1441 TAGCGGGCGC GGGCGAAGCG GTGCGGCGCC GGCAGGAAGG AAATGGGCGG GGAGGGCCTT 1501 CGTGCGTCGC CGCGCCGCCG TCCCCTTCTC CATCTCCAGC CTCGGGGCTG CCGCAGGGGG 1561 ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT GTGACCGGCG 1621 GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA 1681 ACGTGCTGGT TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCGGGCCG GCCGCGTTGA 1741 CGCGCACGGC AAGAGGCGAG GGGCGGCGAC TGGTGAGAGA TGGGTGCGAG AGCGTCAGTA 1801 TTAAGCGGGG GAGAATTAGA TCGATGGGAA AAAATTCGGT TAAGGCCAGG GGGAAAGAAA 1861 AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC TAGAACGATT CGCAGTTAAT 1921 CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC TGGGACAGCT ACAACCATCC 1981 CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA CAGTAGCAAC CCTCTATTGT 2041 GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT TAGACAAGAT AGAGGAAGAG 2101 CAAAACAAAA GTAAGAAAAA AGCACAGCAA GCAGCAGCTG ACACAGGACA CAGCAATCAG 2161 GTCAGCCAAA ATTACCCTAT AGTGCAGAAC ATCCAGGGGC AAATGGTACA TCAGGCCATA 2221 TCACCTAGAA CTTTAAATGC ATGGGTAAAA GTAGTAGAAG AGAAGGCTTT CAGCCCAGAA 2281 GTGATACCCA TGTTTTCAGC ATTATCAGAA GGAGCCACCC CACAAGATTT AAACACCATG 2341 CTAAACACAG TGGGGGGACA TCAAGCAGCC ATGCAAATGT TAAAAGAGAC CATCAATGAG 2401 GAAGCTGCAG AATGGGATAG AGTGCATCCA GTGCATGCAG GGCCTATTGC ACCAGGCCAG 2461 ATGAGAGAAC CAAGGGGAAG TGACATAGCA GGAACTACTA GTACCCTTCA GGAACAAATA 2521 GGATGGATGA CACATAATCC ACCTATCCCA GTAGGAGAAA TCTATAAAAG ATGGATAATC 2581 CTGGGATTAA ATAAAATAGT AAGAATGTAT AGCCCTACCA GCATTCTGGA CATAAGACAA 2641 GGACCAAAGG AACCCTTTAG AGACTATGTA GACCGATTCT ATAAAACTCT AAGAGCCGAG 2701 CAAGCTTCAC AAGAGGTAAA AAATTGGATG ACAGAAACCT TGTTGGTCCA AAATGCGAAC 2761 CCAGATTGTA AGACTATTTT AAAAGCATTG GGACCAGGAG CGACACTAGA AGAAATGATG 2821 ACAGCATGTC AGGGAGTGGG GGGACCCGGC CATAAAGCAA GAGTTTTGGC TGAAGCAATG 2881 AGCCAAGTAA CAAATCCAGC TACCATAATG ATACAGAAAG GCAATTTTAG GAACCAAAGA 2941 AAGACTGTTA AGTGTTTCAA TTGTGGCAAA GAAGGGCACA TAGCCAAAAA TTGCAGGGCC 3001 CCTAGGAAAA AGGGCTGTTG GAAATGTGGA AAGGAAGGAC ACCAAATGAA AGATTGTACT 3061 GAGAGACAGG CTAATTTTTT AGGGAAGATC TGGCCTTCCC ACAAGGGAAG GCCAGGGAAT 3121 TTTCTTCAGA GCAGACCAGA GCCAACAGCC CCACCAGAAG AGAGCTTCAG GTTTGGGGAA 3181 GAGACAACAA CTCCCTCTCA GAAGCAGGAG CCGATAGACA AGGAACTGTA TCCTTTAGCT 3241 TCCCTCAGAT CACTCTTTGG CAGCGACCCC TCGTCACAAT AAAGATAGGG GGGCAATTAA 3301 AGGAAGCTCT ATTAGATACA GGAGCAGATG ATACAGTATT AGAAGAAATG AATTTGCCAG 3361 GAAGATGGAA ACCAAAAATG ATAGGGGGAA TTGGAGGTTT TATCAAAGTA GGACAGTATG 3421 ATCAGATACT CATAGAAATC TGCGGACATA AAGCTATAGG TACAGTATTA GTAGGACCTA 3481 CACCTGTCAA CATAATTGGA AGAAATCTGT TGACTCAGAT TGGCTGCACT TTAAATTTTC 3541 CCATTAGTCC TATTGAGACT GTACCAGTAA AATTAAAGCC AGGAATGGAT GGCCCAAAAG 3601 TTAAACAATG GCCATTGACA GAAGAAAAAA TAAAAGCATT AGTAGAAATT TGTACAGAAA 3661 TGGAAAAGGA AGGAAAAATT TCAAAAATTG GGCCTGAAAA TCCATACAAT ACTCCAGTAT 3721 TTGCCATAAA GAAAAAAGAC AGTACTAAAT GGAGAAAATT AGTAGATTTC AGAGAACTTA 3781 ATAAGAGAAC TCAAGATTTC TGGGAAGTTC AATTAGGAAT ACCACATCCT GCAGGGTTAA 3841 AACAGAAAAA ATCAGTAACA GTACTGGATG TGGGCGATGC ATATTTTTCA GTTCCCTTAG 3901 ATAAAGACTT CAGGAAGTAT ACTGCATTTA CCATACCTAG TATAAACAAT GAGACACCAG 3961 GGATTAGATA TCAGTACAAT GTGCTTCCAC AGGGATGGAA AGGATCACCA GCAATATTCC 4021 AGTGTAGCAT GACAAAAATC TTAGAGCCTT TTAGAAAACA AAATCCAGAC ATAGTCATCT 4081 ATCAATACAT GGATGATTTG TATGTAGGAT CTGACTTAGA AATAGGGCAG CATAGAACAA 4141 AAATAGAGGA ACTGAGACAA CATCTGTTGA GGTGGGGATT TACCACACCA GACAAAAAAC 4201 ATCAGAAAGA ACCTCCATTC CTTTGGATGG GTTATGAACT CCATCCTGAT AAATGGACAG 4261 TACAGCCTAT AGTGCTGCCA GAAAAGGACA GCTGGACTGT CAATGACATA CAGAAATTAG 4321 TGGGAAAATT GAATTGGGCA AGTCAGATTT ATGCAGGGAT TAAAGTAAGG CAATTATGTA 4381 AACTTCTTAG GGGAACCAAA GCACTAACAG AAGTAGTACC ACTAACAGAA GAAGCAGAGC 4441 TAGAACTGGC AGAAAACAGG GAGATTCTAA AAGAACCGGT ACATGGAGTG TATTATGACC 4501 CATCAAAAGA CTTAATAGCA GAAATACAGA AGCAGGGGCA AGGCCAATGG ACATATCAAA 4561 TTTATCAAGA GCCATTTAAA AATCTGAAAA CAGGAAAATA TGCAAGAATG AAGGGTGCCC 4621 ACACTAATGA TGTGAAACAA TTAACAGAGG CAGTACAAAA AATAGCCACA GAAAGCATAG 4681 TAATATGGGG AAAGACTCCT AAATTTAAAT TACCCATACA AAAGGAAACA TGGGAAGCAT 4741 GGTGGACAGA GTATTGGCAA GCCACCTGGA TTCCTGAGTG GGAGTTTGTC AATACCCCTC 4801 CCTTAGTGAA GTTATGGTAC CAGTTAGAGA AAGAACCCAT AATAGGAGCA GAAACTTTCT 4861 ATGTAGATGG GGCAGCCAAT AGGGAAACTA AATTAGGAAA AGCAGGATAT GTAACTGACA 4921 GAGGAAGACA AAAAGTTGTC CCCCTAACGG ACACAACAAA TCAGAAGACT GAGTTACAAG 4981 CAATTCATCT AGCTTTGCAG GATTCGGGAT TAGAAGTAAA CATAGTGACA GACTCACAAT 5041 ATGCATTGGG AATCATTCAA GCACAACCAG ATAAGAGTGA ATCAGAGTTA GTCAGTCAAA 5101 TAATAGAGCA GTTAATAAAA AAGGAAAAAG TCTACCTGGC ATGGGTACCA GCACACAAAG 5161 GAATTGGAGG AAATGAACAA GTAGATGGGT TGGTCAGTGC TGGAATCAGG AAAGTACTAT 5221 TTTTAGATGG AATAGATAAG GCCCAAGAAG AACATGAGAA ATATCACAGT AATTGGAGAG 5281 CAATGGCTAG TGATTTTAAC CTACCACCTG TAGTAGCAAA AGAAATAGTA GCCAGCTGTG 5341 ATAAATGTCA GCTAAAAGGG GAAGCCATGC ATGGACAAGT AGACTGTAGC CCAGGAATAT 5401 GGCAGCTAGA TTGTACACAT TTAGAAGGAA AAGTTATCTT GGTAGCAGTT CATGTAGCCA 5461 GTGGATATAT AGAAGCAGAA GTAATTCCAG CAGAGACAGG GCAAGAAACA GCATACTTCC 5521 TCTTAAAATT AGCAGGAAGA TGGCCAGTAA AAACAGTACA TACAGACAAT GGCAGCAATT 5581 TCACCAGTAC TACAGTTAAG GCCGCCTGTT GGTGGGCGGG GATCAAGCAG GAATTTGGCA 5641 TTCCCTACAA TCCCCAAAGT CAAGGAGTAA TAGAATCTAT GAATAAAGAA TTAAAGAAAA 5701 TTATAGGACA GGTAAGAGAT CAGGCTGAAC ATCTTAAGAC AGCAGTACAA ATGGCAGTAT 5761 TCATCCACAA TTTTAAAAGA AAAGGGGGGA TTGGGGGGTA CAGTGCAGGG GAAAGAATAG 5821 TAGACATAAT AGCAACAGAC ATACAAACTA AAGAATTACA AAAACAAATT ACAAAAATTC 5881 AAAATTTTCG GGTTTATTAC AGGGACAGCA GAGATCCAGT TTGGAAAGGA CCAGCAAAGC 5941 TCCTCTGGAA AGGTGAAGGG GCAGTAGTAA TACAAGATAA TAGTGACATA AAAGTAGTGC 6001 CAAGAAGAAA AGCAAAGATC ATCAGGGATT ATGGAAAACA GATGGCAGGT GATGATTGTG 6061 TGGCAAGTAG ACAGGATGAG GATTAACACA TGGAATTCTG CAACAACTGC TGTTTATCCA 6121 TTTCAGAATT GGGTGTCGAC ATAGCAGAAT AGGCGTTACT CGACAGAGGA GAGCAAGAAA 6181 TGGAGCCAGT AGATCCTAGA CTAGAGCCCT GGAAGCATCC AGGAAGTCAG CCTAAAACTG 6241 CTTGTACCAA TTGCTATTGT AAAAAGTGTT GCTTTCATTG CCAAGTTTGT TTCATGACAA 6301 AAGCCTTAGG CATCTCCTAT GGCAGGAAGA AGCGGAGACA GCGACGAAGA GCTCATCAGA 6361 ACAGTCAGAC TCATCAAGCT TCTCTATCAA AGCAGTAAGT AGTACATGTA ATGCAACCTA 6421 TAATAGTAGC AATAGTAGCA TTAGTAGTAG CAATAATAAT AGCAATAGTT GTGTGGTCCA 6481 TAGTAATCAT AGAATATAGG AAAATGGCCG CTGATCTTCA GACCTGGAGG AGGAGATATG 6541 AGGGACAATT GGAGAAGTGA ATTATATAAA TATAAAGTAG TAAAAATTGA ACCATTAGGA 6601 GTAGCACCCA CCAAGGCAAA GAGAAGAGTG GTGCAGAGAG AAAAAAGAGC AGTGGGAATA 6661 GGAGCTTTGT TCCTTGGGTT CTTGGGAGCA GCAGGAAGCA CTATGGGCGC AGCCTCAATG 6721 ACGCTGACGG TACAGGCCAG ACAATTATTG TCTGGTATAG TGCAGCAGCA GAACAATTTG 6781 CTGAGGGCTA TTGAGGCGCA ACAGCATCTG TTGCAACTCA CAGTCTGGGG CATCAAGCAG 6841 CTCCAAGCAA GAATCCTAGC TGTGGAAAGA TACCTAAAGG ATCAACAGCT CCTAGGGATT 6901 TGGGGTTGCT CTGGAAAACT CATTTGCACC ACTGCTGTGC CTTGGAATGC TAGTTGGAGT 6961 AATAAATCTC TGGAACAGAT CTGGAATCAC ACGACCTGGA TGGAGTGGGA CAGAGAAATT 7021 AACAATTACA CAAGCTTAAT ACACTCCTTA ATTGAAGAAT CGCAAAACCA GCAAGAAAAG 7081 AATGAACAAG AATTATTGGA ATTAGATAAA TGGGCAAGTT TGTGGAATTG GTTTAACATA 7141 ACAAATTGGC TGTGGTATAT AAAATTATTC ATAATGATAG TAGGAGGCTT GGTAGGTTTA 7201 AGAATAGTTT TTGCTGTACT TTCTATAGTG AATAGAGTTA GGCAGGGATA TTCACCATTA 7261 TCGTTTCAGA CCCACCTCCC AATCCCGAGG GGACCCGACA GGCCCGAAGG AATAGAAGAA 7321 GAAGGTGGAG AGAGAGACAG AGACAGATCC ATTCGATTAG TGAACGGATC CTTGGCACTT 7381 ATCTGGGACG ATCTGCGGAG CCTGTGCCTC TTCAGCTACC ACCGCTTGAG AGACTTACTC 7441 TTGATTGTAA CGAGGATTGT GGAACTTCTG GGACGCAGGG GGTGGGAAGC CCTCAAATAT 7501 TGGTGGAATC TCCTACAATA TTGGAGTCAG GAGCTAAAGA ATAGTGCTGT TAGCTTGCTC 7561 AATGCCACAG CCATAGCAGT AGCTGAGGGG ACAGATAGGG TTATAGAAGT AGTACAAGGA 7621 GCTTGTAGAG CTATTCGCCA CATACCTAGA AGAATAAGAC AGGGCTTGGA AAGGATTTTG 7681 CTATAAGCTC GAAACAACCG GTACCTCTAG AACTATAGCT AGCAGATCTT TTTCCCTCTG 7741 CCAAAAATTA TGGGGACATC ATGAAGCCCC TTGAGCATCT GACTTCTGGC TAATAAAGGA 7801 AATTTATTTT CATTGCAATA GTGTGTTGGA ATTTTTTGTG TCTCTCACTC GGAAGGACAT 7861 ATGGGAGGGC AAATCATTTA AAACATCAGA ATGAGTATTT GGTTTAGAGT TTGGCAACAT 7921 ATGCCATATG CTGGCTGCCA TGAACAAAGG TGGCTATAAA GAGGTCATCA GTATATGAAA 7981 CAGCCCCCTG CTGTCCATTC CTTATTCCAT AGAAAAGCCT TGACTTGAGG TTAGATTTTT 8041 TTTATATTTT GTTTTGTGTT ATTTTTTTCT TTAACATCCC TAAAATTTTC CTTACATGTT 8101 TTACTAGCCA GATTTTTCCT CCTCTCCTGA CTACTCCCAG TCATAGCTGT CCCTCTTCTC 8161 TTATGAAGAT CCCTCGACCT GCAGCCCAAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC 8221 TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG 8281 TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC 8341 CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCGGATCCGC ATCTCAATTA GTCAGCAACC 8401 ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT 8461 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT 8521 GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTA 8581 ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA 8641 ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT 8701 ATCATGTCTG GATCCGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG 8761 TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG 8821 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 8881 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 8941 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 9001 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 9061 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 9121 CCCTTCGGGA AGCGTGGCGC TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 9181 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 9241 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 9301 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 9361 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT 9421 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 9481 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 9541 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 9601 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 9661 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 9721 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 9781 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 9841 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 9901 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 9961 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 10021 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 10081 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 10141 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 10201 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 10261 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 10321 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 10381 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 10441 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 10501 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 10561 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 10621 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 10681 ATTTCCCCGA AAAGTGCCAC CTG / psPAX2 其他相关慢病毒载体：其他相关慢病毒载体： Tet-pLKO-neo Tet-pLKO-puro pPACKH1-GAG pMD2.G pCMV-dR8.2-dvpr pLKO.1-GFP-shRNA pLKO.1-TRC control pLKO.1-hygro pLKO.1-TRC pCDH-MSCV-MCS-EF1-copGFP pCDH-MSCV-MCS-EF1-copGFP-T2A-Puro FUW-tetO-hOKMS FUW-tetO-hOCT4 FUW-tetO-hSOX2 FUW-tetO-hKLF4 FUW pLVX-AcGFP1-N1 pLVX-AcGFP1-C1 pLVX-AmCyan1-N1 pLVX-DsRed-Express2-C1 pLVX-DsRed-Express2-N1 pLVX-DsRed-Monomer-N1 pLVX-PAmCherry-C1 pLVX-PAmCherry-N1 pLVX-ZsGreen1-N1 pLVX-IRES-ZsGreen1 pLVX-IRES-mCherry pLVX-mCherry-C1 pLVX-mCherry-N1 pLVX-tdTomato-C1 pLKO.1-puro pLentilox 3.7 pLVX-Tet-On-Advanced pLVX-IRES-Puro pLVX-IRES-Neo pLVX-IRES-Hyg pLVX-EF1-DsRed-Monomer-C1 pLVX-EF1-AcGFP1-N1 pLVX-EF1-AcGFP1-C1 pLVX-EF1-mCherry-C1 pLVX-EF1-IRES-mCherry pLVX-EF1-IRES-ZsGreen1 pLVX-MetLuc Control pLVX-MetLuc pLVX-Hom-Mem1 pLVX-Het-2 pLVX-DD-AcGFP1-Actin pPRIME-TET-GFP-FF3 pSIH1-H1-CopGFP pCDH-EF1-MCS-T2A-Puro pCDH-CMV-MCS-EF1-Puro pCDF1-MCS2-EF1-copGFP pLOX-CWBmi1 pLOX-CW-CRE pRSV-rev pMDLg-pRRE pLL3.7 pLVX-DD-AmCyan1 Control pLVX-DD-AmCyan1 Reporter pLVX-DD-tdTomato Reporter pLVX-DD-tdTomato Control pLVX-PTuner-Green pLVX-CherryPicker2 pLVX-TetOne-Puro-Luc pLVX-Tet