小单孢菌属和拟诺卡氏菌属.ppt

稀有放线菌的分离与培养,报告人：张晓敏,报告内容,分离培养基的选择小单孢菌形态与生理生化特征拟诺卡氏菌形态与生理生化特征小单孢菌、拟诺卡氏菌与链霉菌区别,分离培养基的选择,淀粉-酪蛋白硝酸盐琼脂培养基溶胶几丁质琼脂培养基甘露醇-利福平琼脂培养基,淀粉-酪蛋白硝酸盐琼脂培养基,starch-caseinnitrateagar：starch10g，sodiumcaseinate0.3g，KNO32g，agar15g，PH7.0-7.4.37gnovobiocin，223gmycostatin30培养21天Micromonosporachaiyaphumensissp.novMicromonosporaeburneasp.nov,基础培养基：K2HPO40.7g，KH2PO40.3g，MgSO47H2O0.5g，FeSO47H2O0.01g，ZnSO40.001g，琼脂10克，蒸馏水1.0L.PH7.0121蒸汽灭菌20min,每100mL加20mL胶体几丁质悬浮液和5mg放线菌酮,溶胶几丁质琼脂培养基,甘露醇-利福平琼脂培养基,mannitol-rifampicinagar：甘露醇10g，酵母提取物7g，酪蛋白水解物2g，蛋白胨1g，NaCl1g，CaCO30.2g，制霉菌素100mg,利福平5mg27培养6周Nocardiaacidivoran,返回,Micromonospora,gyrB基因序列分析和DNADNA杂交15species:Micromonosporaaurantiaca,Micromonosporacarbonacea,Micromonosporachalcea,M.chersina,Micromonosporacoerulea,Micromonosporaechinospora,Micromonosporagallica,Micromonosporahalophytica,Micromonosporainositola,M.matsumotoense,Micromonosporanigra,M.olivasterospora,Micromonosporapallida,MicromonosporapurpureochromogenesandM.rosaria.,小单孢菌形态与生理生化特征,Aerobic,Gram-positive,mesophilic,non-motileactinomycetewell-developedandbranchedsubstratehyphae.Aerialmyceliumwasabsent.Sporechainswereabsent.Mycolicacidswereabsent.Acyltypeofcellwallmuramicacidisglycolyl.Fatty-acidtype:3b.Thepredominantmenaquinone:MK-9（H4）、MK-9（H4H6H8）Characteristicwhole-cellsugarsarexyloseandarabinose.Phospholipids:P-II型.Cell-wallpeptidoglycan:CW-II(glutamicacid,alanineandmeso-diaminopimelicacid).DNAG+C(mol%):71%-73%.,well-developedandbranchedsubstratehyphae.Aerialmyceliumwasabsent.Atmaturity,sporesweresphericaltooval,appearedtoberoughandwerenon-motile(Fig.1).colourofthevegetativemyceliumonISP2mediumisorange,turningtogreyishblackorblackuponsporulation.,growthatpH4-8and25-30,nogrowthabove45Brown,diffusiblepigmentpositivefortheutilizationofL-arabinose,D-fructose,D-galactose,D-glucose,lactose,melibiose,salicinandD-xylose.negativefornitratereductionandtheutilizationofD-mannitolandrhamnose.,Micromonosporachaiyaphumensis,Single,non-motile,sphericalspores(0.60.9mm)wereformedonsubstratehyphae.Aerialmyceliumisabsent.Thesporesurfaceappearswarty.Sporesarenon-motile.,Thecolourofthespulatingcolonieswasyellowishwhitetopaleorange.Ayellow,solublepigmentUtilizesL-arabinose,D-fructose,D-galactose,D-glucose,lactose,D-ribose,salicinandD-xylose;weakutilizationofcellobiose,notutilizeglycerol,D-mannitol,melibiose,raffinoseorL-rhamnose.,Micromonosporapattaloongensis,well-developedandbranchedsubstratehyphae.Single,non-motilesporesareformedonsubstratehyphae，0.45mmindiameter.Aerialmyceliumisabsent.Thespore-surfaceappearsroughandnodular.ColoniesareyellowishwhiteandturngreyishblackaftersporulationPaleyellowsolublepigmentpositivefornitratereduction,UtilizesD-glucose,L-rhamnose,D-melibiose,D-rafnose,glycerol,salicin,lactose,cellobioseandD-xylose;weaklyutilizesD-mannitolandD-galactosebutnotL-arabinose,D-fructoseorD-ribose.,Micromonosporaeburnea,生理生化区别,返回,拟诺卡氏菌属,原拟诺卡氏菌属包括7个种2个亚种.Grund等根据化学分类和数值分类研究