人类疱疹病毒1精美生物医学.ppt

人类疱疹病毒,Humanherpesviruses,ContainsseveralofthemostimportanthumanpathogensAspectrumofdiseasesLifelongpersistentinfectionPeriodicreactivation,Mainproperties,Virion:spherical,150-200nmcore+capsid+envelopedsDNA,encodesatleast100proteins35proteinsstructureofvirusReplication:InthenucleusBudfromnuclearmembraneEarlyreplicationandlatereplication,CPE:apartfromEBV,otherscancauseCPEincellcultureFormofinfectionReplicationinfection:primary,reactivatedLatentinfectionCongenitalinfection(先天性感染)Celltransformation,Herpessimplexvirus(HSV),PropertiesTwoserotypes:HSV1andHSV2HSVgenome:encodesatleast70proteinssomeassociatedwithviralreplicationsomeenvelopeproteinsassociatedwithimmunereactionviralpenetration,Herpessimplexvirus（HSV）,Pathogenicity,SourceofinfectionpatientsandcarriersRoutesoftransmissionHSV1：contactHSV2：sexually/maternalgenitalinfectionPathology:cytolyticinfection,Primaryinfectionmostareinapparent,butresultinantibodyproductionandlatentinfection,HSV1Gingivitis(齿龈炎):mostfrequentlyKeratoconjunctivitis(角膜结膜炎)SkininfectionEncephalitisLatentinfection:inthetrigeminalganglia(三叉神经节),HSV2Genitalherpes:commonseenSkinherps:asHSV1Neonatalherpes:duringbirth,isaseverediseaseskin,eye,monthlesionencephalitismultipleorgandisseminated(多器官感染)Latentinfection：sacralganglia(骶神经节),LatentandrecurrenceinfectionHSV1,HSV2neuronvirusreactivate,replicationaxon(神经纤维)peripheralsites,skinormucousmembraneHSV1：口唇部疱疹HSV2：genitalherpes,Varicella-zostervirus（VZV）水痘带状疱疹病毒,similarasHSV,VZVnaked,VZVenveloped,PathogenicitySourceofinfection：patientsRoutesofinfection:respiratorytractDisease：水痘(Varicella/chickenpox)：儿童：斑丘疹，水疱，偶见并发症孕妇：症状较重，可致胎儿畸形，流产，死胎免疫低下：重症水痘Latentinfectiondorsalrootganglia(脊髓后根神经节),Zoster(带状疱疹)SeeninadultsSeverepainandvesicle,ofteninchest,Cytomegalovirus(CMV)巨细胞病毒,ThelargestgeneticcontentofHHVVeryspeciesspecificandcellspecific(种属和细胞特异性)CharacteristicCPE:multinucleatedcellsandintranuclearinclusionIsspreadcelltocell,Pathogenicity,Sourceofinfection:patients/inapparentpatientsRoutesofinfectionClosecontactMothertochild,Diseases,NormalhostMostaresubclinicalMononucleosislikesyndrome(单核细胞增多症)amildillnessLatentinfectionImmunosuppressedhostMuchmoresevereDisseminatedpneumoniaInAIDS:胃肠炎，视网膜炎,Congenitalandperinatedinfection先天和围产期感染MaybesevereFetalandnewbornCytomegalicinclusiondisease(巨细胞包涵体病)DeathoffetusC.N.Sdefect:耳聋，智力发育迟缓Animportantpublicproblem,Epstein-BarrvirusEBV,ViralantigensLatentphaseAgEBNA1:latentinfectionEBNA2:transformationofcellsLMP:latentinfectiontransformcellsReplicationAgEarlyAg:viralreplicationLateAg:structureproteins,Pathogenecity,Spearedbycontact:Primaryinfection:moreunapparentLatentinfectionCelltransformation,DiseasesInfectiousmononucleosisTransmittedbyinfectedsalivaMoreseeninadolescentsandyoungadultsFever,sorethroat,WBCincraesedDistinguishwithleukemiaheterophilagglutination(异嗜凝集试验)：heterophilAbcanagglutinatesheepRBCBurkittslympho