大鼠颈静脉采血PPT课件.ppt

1,JugularBloodCollectioninaConsciousRat,.,2,Calmtheratdownbeforerestrainingbygentlystrokingtheanimal.,3,4,Restraintheratbygraspingtheskinoverthenapeoftheneckandshoulderregionwithyourthumbandindexfinger(scruffing).,5,6,Onceyouhavescruffedtheneck,gentlyturnyourhandoversotheratislyingcomfortlyinthepalmofyourhand.Donotsqueezetherataroundthethoraxregionbecausethatcouldcausedifficultybreathing.Makesuretheratsbodyisstraightandnotbentorcurledinyourpalm.,7,8,Withyourotherhand,useindexfingertolightlyholdheadoutofway.Donotputtomuchpressureonratsheadorneck.Withthumb,holdforelimbsback,whileanothertechnicianshavesthearea.,9,10,11,Haveothertechnicianwipedowntheshavedareawithanalcoholswab.,12,Withyourotherhand,useindexfingertolightlyholdhead.Donotputtomuchpressureonratsheadorneck.Withthumb,applyslightpressureonveinproximaltothecollectionsitesoitwilldistend.Thiswillmakethejugularveinmoreprominentforeasiervisualization.Thebottomhalfofratisstilljustrestinginpalmofhand.,13,Havethetechniciandrawingbloodgetthesyringeandneedleready.Weusea1mlsyringewitha23gaugeneedle.,14,Insertneedlethroughskinrightaroundthemiddlepointbetweenthesternumandshoulderarea,whereyoushouldseethevein.Advanceslowly.Createaslightvacuumwithinthesyringe,whileadvancingyourneedle.,15,Onceyouhitthevein,asmalldropofbloodwillleakintothehuboftheneedle.Trynottoletyoursyringemovearoundandkeepdrawingbackontheplunger.Donotpullbacktoofastbecauseitmightcausetheveintocollapse.,16,17,Onceyouhave