生物化学 复习资料 重点+试题 第三章 酶与辅酶.doc

SETpHAIKmKmVmaxAsp102His57Ser195 1Km2substrate specificity3prosthetic group4monomeric enzyme5oligomeric enzyme6multienzyme system7activator8inhibitor inhibiton9allosteric enzyme10isozyme11induced enzyme12zymogen13enzymatic compare energy14active center1NAD+nicotinamide adenine dinucleotide2FADflavin adenine dinucleotide3THFAtetrahydrofolic acid4NADP+nicotinamide adenine dinucleotide phosphate5FMNflavin mononucleotide6CoAcoenzyme A7ACPacyl carrier protein8BCCPbiotin carboxyl carrier protein9PLPpyridoxal phosphate1 2 3 4 5 6 71 2 VS 8 9 10Asn-His-Lys-Asp-Phe-Glu-Ile-Arg-Glu-Tyr-Gly-Arg 11 12 13TRCechS.Alman 198914 15EC1.1.1.1EC 4 16 17 18 19 20 21 22pH 23 24 -1- 25Lineweaver-Burk 26 27 28_29_30B1_31B2_32B3_33B5_34_35B12_36C_1A BC DE2A B CD E3A BC DE4A B CD E5SA B CD E6AVmaxKm BVmaxKmCVmaxKm DVmaxKmEVmaxKm7A B C D E 8A D 9H+H+AHis BLys CArgDCys ETrp1050%I=Ki A BC D E11ACoA BCoQ CPLP DFH2 EFMN12ABCBDB13AB1 BB2 CB5DB6 EB714AB1 BB2 CB5DB6 EB715AB1 BB2 CB5DB6 EB716ACoA BATP C D17A B C D E18ACoA BFMN CFAD DNAD+ ENADP+19B6A B CD E 1 2 3Km 4V-SS 5 6 7Vmax 8-SH 9 10 11 12 13 14Km=610-6mol/LKm=210-3mol/L 15Km 16KmKm 17Km 18Km 19SKm VVmaxEV 20pHpH 21 22 23 24 251g10mg100 26pH 27B 28B 29 30 31 32E123CechAltmanRNA41NH2CONH2NH2CONHCH32-D-D-3R1COOR24L-L-D-56-1-525mg25mL2mL0.2mg0.1mL1500g11g126VmaxVSVV/SS/VSVmaxKm7128S mmol/L 2.0 3.0 4.0 10.0 15.0 mol 13.9 17.9 21.3 31.3 37.0mol 8.8 12.1 14.9 25.7 31.39-3-Mr 150 000Cys5mL1.2mg/mL3.010 -2mgMr 18510KmKmKm11121S=KmV=35mol/minVmaxmol/min2S=210 -5mo/LV=40mol/minKm3IKI=410-5mol/LS=310-2mol/LI=310-5mol/LV4IKISI3V2S=1.010-6mol/LS=1.010-1mol/Lv3S=2.010-3mol/LS=2.010-6mol/L5min44KmVmax13His22BABCDEFG1B1 2B2 3B3 4B5 5B6 6B7 7B11 8B12()FMN()FAD()NAD+()NADP+()CoA()PLP()PMP()FH2FH4()TPP1KmmmVVmaxMmM23413,00035,000535 000678910111213= mg14 1NAD+ nicotinamide adenine dinucleotide2FADflavin adenine dinucleotide3THFAtetrahydrofolic acid4NADP+nicotinamide adenine dinucleotide phosphate5FMNflavin mononucleotide6CoAcoenzyme AA7ACPacyl carrier protein8BCCPbiotin carboxyl carrier protein9PLPpyridoxal phosphate123ESpHTIA4Ser195His57Asp102Ser195567S8VB69DHFATHFA10111213RNA1415-CHOHNAD+NADP+1161718195%202122232425-1/Km1/Vmax2627282930TPP3111032-3-AM