遗传学实验报告.doc

abstract: in this experiment, we chose allium sativum as the material. we got a lot of activity floristic cells through cultivating the roots of allium sativum. then we disposed the cells in order to observe the whole processes of the mitosis.key words: mitosis interphase prophase metaphase anaphase telophaallium sativum 2n=16carnoy(95%31)1mol/lhcl1. 42.0cm1000,14h2. 1mol/lhcl60,.4min5hcl3. 1/35min -1interphase-1(a)prophase-1(b)metaphase-1(c)anaphase-1(d) telophase-1(e)190011001500170024min31.5mm456 abstract: in this experiment, we chose zea mays as the material. we got a lot of cells in meiosis. then we disposed the cells in order to observe the whole processes of the meiosis.key words: meiosis leptonema zygonema pachynema diplonema diakineses(zea mays 2n=20)carnoy(95%31)1 8301030200400carnoy2 ,3 4%424h0.5%424h254 45%5-21 2 3 4 5 6 abstract: in this experiment, we chose allium sativum as the material. we got a lot of activity floristic cells through cultivating the roots of allium sativum. then we put colchicine into the water. after about 36 hours, we disposed the polyploid cells in order to observe themselves. key words: polyploidy colchicineallium sativum 2n=16carnoy(95%311mol/lhcl 1 2.0cm0.13648h2 3 5min-3a (b)-3(a)2n=16(b)4n=321 2 322n324n323 4 9001100150017005 4min6 1.5mm7 8 9 drosophila melanogaster2n=8 abstract: drosophila melanogaster is a classic material of studying genetics. as we all know, drosophila melanogaster has a 2n number of 8, which means we could easy to observe it. especially for its larval salivary chromosome, it is very huge and clear. so it is called huge chromosome. in this experiment through the observation of the salivary chromosome to explore the structure of chromosome.key words: drosophila melanogaster salivary chromosome100-200,drosophila melanogasterhcl(1mol/l)1 2325,2 1/33 1mol/lhcl234 345 56 51. -11 2 1/33 4 drosophila melanogaster10142n=8 abstract: drosophila melanogaster is a classic material of studying genetics. firstly, it is easy for us to collect and to feed. secondly, as we all know, drosophila melanogaster has a 2n number of 8, which means we could easy to observe it, furthermore, it also has numerous of gene mutations. finally, its biocycle is very shorttherefore it can generate a generation in only 10-14 days. in this experiment we performed the three-point mapping in drosophila melanogaster.key words: drosophila melanogaster three-point mappingdrosophila melanogaster+wmsn1 782 3 5 ()3 35254 78f15 f1 45 f16 f13525787 f2f2 1 f2f2862 m-sn sn-w m-w+ + + 102 0 0 0+ + sn 10 10 10 0+ m + 29 29 0 29+ m sn 22 0 22 22w m sn 42 0 0 0w + + 42 0 42 42w + sn 27 27 0 27w m + 19 19 19 08593120 2932 1 f12 3 4 mus musculus 2n=40 abstract: mus musculus , whose cells in bone marrow have a powerful energy in division,is a classic material in learning genetics. in this experiment, we chose the bone-marrow-cell as the material. we got a lot of activity cells from its bone marrow. then we disposed theh cells in order to observe the structure of the chromosome.key words: bone marrowchromosomemus musculusgiemsa1:11 10mg100ml8.5gnacl1000ml0.85%5.59gkcl1000ml0075%2 5g2h3 2%2%2%4 1000r/min10min5 5ml3720min6 1000r/min10min15min,7 1:12-38 21/32/39 1:10giemsa10min10 -12n=401 2 1m3 4 15min giemsa abstract: there are a lot of homeocyte in human blood, which can access to cell division when given a irritation. in this experiment, we chose human blood cell as the material. we got a lot of activity cells from it. then we disposed theh cells in order to observe the structure of the chromosome.key words: blood cell chromosomepha giemsapbspha, , , , m199, -(31 1 pha2 m199 4ml1ml0.04ml3-4pha0.3ml3 0.2-0.3ml4 3772h5 68h44h6 1000r/ 8370.075m kcl,37207 1ml-311000r/78 5ml151000r/79 10 1-311 giemsa1-2giemsa1:1015-22n=461 1m2 3 15min4 5 g abstract: the technology of detaching chromosome band is an important method to identify each chromosome or c