支原体污染的预防.ppt

BIOLOGICALINDUSTRIES DOYOUSUSPECTMYCOPLASMA Prevention DetectionandTreatmentofMycoplasmaContamination支原体污染的预防 检测与治疗 BiologicalIndustriesoffersyou MYCOPLASMADETECTION支原体检测 EZ PCRMycoplasmaTestKitCat 20 700 10 10assays Cat 20 700 20 20assays ReadytousePCRmixforthedetectionofmycoplasmaincellculture即用型细胞培养中支原体检测PCR试剂盒 EZ PCRMycoplasmaTestKitisdesignedtodetectthepresenceofmycoplasmacontaminatingbiologicalmaterials suchasculturedcells EZ PCR支原体检测试剂盒用来检测生物材料 如培养的细胞中支原体的存在 Mycoplasmadetectionbythedirectcultureprocedureistime consumingandsomemycoplasmaspeciesaredifficulttocultivate 直接培养方法检测耗时且有的种类支原体很难培养 WithPCRtesting resultsareobtainedwithinafewhours sincethepresenceofcontaminantmycoplasmacanbeeasilydetectedsimplybyverifyingthebandsofamplifiedDNAfragmentsinelectrophoresis 用PCR检测 几小时可出结果 支原体DNA片段可通过电泳条带确认 Theprimersetallowsdetectionofvariousmycoplasmaspecies withhighsensitivityandspecificity 系列引物可以高特异性和高灵敏度地检测很多种支原体 TREATMENTOFMYCOPLASMA INFECTEDCELLSWITHANTIBIOTICS用抗生素治疗支原体污染的细胞 Thecontaminationofcellswithmycoplasmaisaverycommonproblem eventhoughitoftengoesunnoticedsincenocloudinessappearsinthecellculture 支原体污染细胞培养是很常见的问题 常常因其不造成混浊而不被注意到 Sincemycoplasma infectedcellscannotalwaysbediscarded manycomplicatedmethodshavebeensuggestedfortheeliminationofthemycoplasma 支原体污染的细胞有时不能废弃 有许多复杂的方法可以用来去除污染 BiologicalIndustriesisnowofferingacombinationofantibiotics whichhavebeenshowntobeeffectiveintheeliminationofmycoplasmaspeciesthataccountfor90 ofthecontaminationfoundincellcultures BiologicalIndustries的抗生素系列产品能有效杀灭细胞培养中的90 以上的支原体 BIOMYC 3 cat 03 038 1 BIOMYC 1 cat 03 036 1 BIOMYC 2 cat 03 037 1 BIOMYC 1isbasedontheantibiotictiamutin whichisproducedbythefunguspleurotusmutilus 成分为泰妙菌素 BIOMYC 2isbasedonminocycline whichisatetracyclinederivative 二甲胺四环素 四环素衍生物 Thesetwoantibioticsolutionsaregenerallyusedsequentiallyincombination 以上抗生素溶液一般按顺序联合使用 BIOMYC 1 BIOMYC 2 BIOMYC 3 BIOMYC 3isbasedontheciprofloxacinantibiotic whichisamemberofthefluoroquinolonegroup ManymycoplasmaspecieshavebeenfoundtobesensitivetoBIOMYC 3 BIOMYC 3成分为氟喹酮类的环丙沙星 多种支原体对其敏感 PREVENTION PHARMACIDAL cat IC 110100 Disinfectantsolutionforincubatorsandsterilework benchesincellculture andmolecularbiologylaboratories用于消毒容器的表面 AQUAGUARD 1 cat 01 867 1 DisinfectingofCO2incubatorwaterbaths二氧化碳培养箱水槽专业抗支原体污染试剂 AQUAGUARD 2 cat 01 916 1E DisinfectingofordinaryWaterbaths一般培养箱水槽抗支原体处理试剂 PHARMACIDAL Theproblemofcontaminationinincubatorsand orsterilework benchesisoftenaseriousone leadingtoextensivedamage Non toxicandbiodegradable andthereforecanbeusedforsprayingincubatorswhilethecellsremaininsidePreventscontaminationwithandgrowthoffungi andspores bacteria andspores mycoplasmaandviruses includingHIVandHepatitisB Activeingredients QuarternaryBenzylammoniumcompounds Thesolutiondoesnotcontainmercury formaldehyde phenoloralcohol 培养箱 无菌台的污染常导致更广泛的破坏 无毒和可降解性 可喷洒正进行细胞培养的培养箱 防止真菌 芽孢 细菌 芽孢 支原体和病毒 HIV HBV 污染 活性成分为四苯甲基化合物 不含汞 甲醛 苯酚 AQUAGUARD 1FordisinfectingCO2incubatorwaterbaths Thewaterrequiredtocreatethehumidityisasourceofcontaminationwhichdispersesintheincubator InordertodisinfectthewaterwerecommendAquaguard 1Solution whichcontainsadisinfectantthatdoesnotcausedamagetothestainlesssteeltray isnon toxic non volatile andextremelyeffective Thewatershouldbereplacedwithsterilewatereverytwotofourweeks adding50mlofAquaguard 1per5litersofwater 保持湿度的培养箱中的水是污染来源之一 消毒培养箱中的水 建议使用Aquaguard 1溶液 它不会腐蚀不锈钢盘 无毒 不挥发且极有效 水应每两到四周更换一次 每5升水加入50mlAquaguard 1溶液 AQUAGUARD 2Forpreventionofmicrobialgrowthinordinarywaterbaths用于防止普通水浴槽的微生物生长 Aquaguard 2isintendedfordisinfectingvariouskindsofwaterbathsfrombacteriaandfungi Aquaguard 2用来消毒各种水浴箱Itisrecommendedtouse2mlofAquaguard 2foreachliterofwaterinthebath andtorepeattheprocedureevery1 2weeks After4 6weeks thebathshouldbeemptiedandrefilledwithwatercontainingAquaguard 2 使用浓度为2ml L 每1 2周重复一次 4 6周后彻底更换含Aquaguard 2的水TheactiveingredientinAquaguard 2issafetohumansanddoesnotcauseanyirritatingeffectstotheskinwhenusedintherecommendedconcentration Aquaguard 2isbiodegradable Aquaguard 2的有效成分在推荐使用浓度时对人体安全 且可降解 MYCOPLASMAFAQ s常见问题 Whyisroutinemycoplasmatestingimportant 为什么定期支原体检测很重要 Thecellfunctionsofmycoplasma infectedcellsareaffected Sinceitisdifficulttodetectinfectionreliablywithothermethods itispossiblethatresultsofexperimentsarebeingmisinterpreted 支原体可改变细胞功能 用其他方法很难检测出感染 进而导致实验结果被曲解 DoIhavetoculturemycellsintheabsenceofantibioticsbeforetesting 检测前细胞培养中不能使用抗生素吗 Ifitisnotanurgentcase itisrecommendedtoculturewithoutantibioticsforseveraldays inordertomaximizethestrengthofthesignal thatisobservedinPCR 建议在没有抗生素的情况下培养几天 以便最大化PCR观测信号 CanItestcellsimmediatelyaftertheyhavebeenremovedfromliquidnitrogen 细胞从液氮中取出后能够立即检测吗 Itisrecommendedtoletthemfirstgrowuntil90 100 confluencewithoutantibiotics 建议在无抗生素条件下生长至90 100 融合后 HowfrequentlyshouldItestmycells 检测频率 WhenevernewcellsenterthelabBeforeeachliquidnitrogenstorageRoutinelyevery2 3monthsWeeklyaftercontaminationhasbeenfoundinthelaboratoryWhenyoubelievethatcellscharacteristicshavebeenmodified新细胞进入实验室液氮保存前定期每2 3个月发现污染后每周发现细胞特性发生改变 Whichantibiotic s shouldIusetotreatinfectedcells 抗生素的选择 Biomyc 1 Biomyc 2sequentially orBiomyc 3aloneBiomyc 1 Biomyc 2按顺序联合使用 Biomyc 3单独使用Biomyc 1 Biomyc 2 Biomyc 3togethercover90 ofthespecies三种同时能去除90 支原体Thereisnosingleantibioticthatcoversthewholespectrum没有一种抗生素能去