骨髓间充质干细胞修复椎动脉型颈椎病的血管损伤.doc

骨髓间充质干细胞修复椎动脉型颈椎病的血管损伤张夏梦1，寿折星21辽宁医学院附属第一医院中医科，辽宁省锦州市 1210002华中科技大学同济医学院协和医院中医科，湖北省武汉市 430022Bone marrow mesenchymal stem cells repair vascular injury after cervical spondylotic vertebral arteriopathyZhang Xia-meng1, Shou Zhe-xing21Department of Traditional Chinese Medicine, First Affiliated Hospital of Liaoning Medical University, Jinzhou 121000, Liaoning Province, China2Department of Traditional Chinese Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China全文: PDF (1970 KB) 输出: BibTeX | EndNote (RIS) 摘要 背景：炎症刺激是椎动脉型颈椎病发病的重要因素，骨髓间充质干细胞具有很强的免疫调节作用，能有效抑制炎症，具有治疗椎动脉型颈椎病的潜能。目的：探讨椎动脉型颈椎病血管损伤机制及骨髓间充质干细胞的治疗作用。方法：40只日本大耳兔随机分为4组：空白组不作任何处理；模型组、丹参酮组、干细胞组采用硬化剂注射法制作椎动脉型颈椎病模型。造模24 h后，空白组，模型组不进行干预，丹参酮组和干细胞组经耳缘静脉注射丹参酮A磺酸钠溶液10 mL和骨髓间充质干细胞混悬液10 mL，干预2周后取材。结果与结论：与模型组相比，干细胞组管壁中膜平滑肌细胞肥大增生明显受到抑制，血管内皮皱折较匀称；干细胞组与丹参酮组比较无明显差别。与模型组相比，干细胞组椎动脉血管cathepsin B、白细胞介素6、肿瘤坏死因子表达显著下降，差异有显著性意义(P 0.05)。结果可见炎症反应可能是椎动脉血管受到损伤的机制之一；骨髓间充质干细胞能有效抑制椎动脉血管炎症反应，修复受损椎动脉血管。中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程全文链接：窗体顶端关键词 ： 干细胞； 骨髓干细胞； 骨髓间充质干细胞； 椎动脉型颈椎病； cathepsin B； 白细胞介素6； 肿瘤坏死因子； Abstract： BACKGROUND: Inflammation is an important factor in cervical spondylotic vertebral arteriopathy, bone marrow mesenchymal stem cells have the potential to treat cervical spondylotic vertebral arteriopathy because of the immunomodulatory effects to inhibit inflammation.OBJECTIVE: To investigate the injury mechanism of vascular injury in the model of cervical spondylotic vertebral arteriopathy and to study the therapeutic effects of bone marrow mesenchymal stem cells on it. METHODS: Forty Japanese big ear rabbits were randomly divided into four groups: control group, model group, tanshinone group, and stem cell group. After modeling, the control and model groups were not given intervention, while the tanshinone and stem cell groups were injected with tanshinone II A sodium sulfonate solution (10 mL) and bone marrow mesenchymal stem cell suspension (10 mL) along the ear vein, respectively. After 2 weeks, the routine pathological examination was done to observe the vascular morphological changes, immunofluorescence staining was done to observe the cathepsin B expression in the vertebral artery, and ELISA was used to detect the expression of tumor necrosis factor- and interleukin-6 in the vertebral artery.RESULTS AND CONCLUSION: Compared with the model group, the arterial smooth muscle cell hypertrophy and hyperplasia was obviously restrained in stem cell group, and vascular endothelial fold was in symmetry, while no significant difference was found between stem cell group and tanshinone group. Compared with the model group, expressions of cathepsin B, interleukin 6 and tumor necrosis factor- expression were reduced significantly in the stem cell group (P 0.05). Inflammatory reaction may be one of mechanisms for vertebral artery damage, and bone marrow mesenchymal stem cells can effectively inhibit inflammation of the vertebral artery and improve vascular remodeling.中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程全文链接：Key words： bone marrow; mesenchymal stem cells; cervical vertebrae; interleukin-6; tumor necrosis factor-alpha; cathepsin B; 窗体底端 中图分类号:R394.2 通讯作者