非小细胞肺癌 NLK 细胞周期 增殖 凋亡.doc

非小细胞肺癌论文：NLK-shRNA对H1299肺腺癌细胞增殖抑制的实验研究【中文摘要】肿瘤是一类细胞周期病。细胞周期调控失常、细胞增殖失控被认为是恶性肿瘤发生的重要机制；前期研究认为,NLK与多种肿瘤的增殖与凋亡密切相关。本研究拟证实在非小细胞肺癌中阻断内源性NLK的表达对NSCLC细胞生物学行为的影响,为进一步研究NSCLC发病分子机理提供科学依据。方法：Westemblotting验证NLK在NSCLC细胞系：A549、H1299、NCI-H1581、NCI-H522及SK-MES-1中的表达情况；利用慢病毒载体系统构建NLK-shRNA干扰载体,感染H1299肺腺癌细胞系,获得NLK干扰细胞系,并以此为研究对象,利用real-time PCR验证慢病毒感染后NLK的表达水平；细胞增殖实验、克隆形成实验、流式细胞周期检测及凋亡实验研究NLK与NSCLC细胞增殖、凋亡的生物学行为的关系。结果：Westemblotting结果显示：NLK在A549、H1299、NCI-1581、NCI-H522及SK-MES-1五株非小细胞肺癌细胞系中均有表达；NLK-shRNA慢病毒干扰载体感染NSCLC H1299细胞系后,real-time PCR检测结果显.【英文摘要】:Aberration of cell cycle regulation and cell proliferation are considered to be the main cause of malignant tumors. Previous studies have indicated that nemo-like kinase (NLK) is closely related to cell proliferation in different kinds of human cancers. However, the underlying mechanism is still unclear. The aim of current study is to confirm whether targeted disruption of NLK would inhibit cell proliferation and promote apoptosis of NSCLC cells, which would provide scientific basis for a furt.【关键词】非小细胞肺癌 NLK 细胞周期 增殖 凋亡【英文关键词】non-small cell lung cancer NLK cell cycles proliferation apoptosis【索购全文】联系Q1：138113721 Q2：139938848【目录】NLK-shRNA对H1299肺腺癌细胞增殖抑制的实验研究中文摘要5-7英文摘要7-8缩略词表9-10NLK-shRNA对H1299肺腺癌细胞增殖抑制的实验研究10-41前言10-12材料12-15方法15-25结果25-33讨论33-37结论37-38参考文献38-41文献综述