药学专业外语丹参ppt课件.ppt

丹参 RadixSalviaeMiltiorrhizae MainContents OriginsPlantMorphologyCollectionandPreparationProductionandCommerceDescriptionMicroscopicalCharactersChemicalConstituentsPhysicalandChemicalIdentificationHighPerformanceLiquidChromatography 2 PlantMorphology Aperennialherb denselypubescentandglandularhairythroughout Rootcylindrical vermillion Stem4 angled branchedattheupperpart Leavesopposite pinnatelycompound leaflets5 7 ovateorelliptical ovate serratealongthemargin pubescentonbothsurfaces vermillion 朱红色serrate 锯齿状的 3 Flowersinterminaloraxillaryverticillatepseudo racemes calyxbell shaped corollabluish purple Theupperliperect slightlyfalcate retuseatapex thelowershorter 3 lobed themiddlelobelarger 2 cleft stamens2 filamentsshort connectiveslong theupperendoftheconnectivebearingafertileanther cell thelowerendwithanimperfectone ovarydeeply4 lobed stylegynobasic Nutlets4 ellipsoid verticillate 轮生angled 棱pseudo 假的retuse 浅凹形falcate 钩状的filament 花丝style 花柱anther 花粉囊gynobasic 着生于基部的 4 Description Rootcylindricalorconical usuallycurved branched 10 30cmlong 0 3 1 5cmindiameter Externallybrick red brownish redorpurplish brown rough longitudinallyfurrowedorwrinkled thebarkofoldrootsmostlyscalingoff sometimescrownedwithremainsofstembase conical 圆锥形的 5 Texturehardandfragile fracturefibrous barkdarkreddish browntopurplish brown woodshowingbundlesofvessels yellowish white arrangedradially Odour slight tasteslightlyastringent fragile 脆的astringent 收敛性的 6 Transversesection Corkconsistingofseverallayersofcells mostlycontainingorangeorpurplish browncontents Phellodermnarrow Phloemrelativelybroad Cambiumringdistinct Xylembundlesradial consistingofvessels xylemfibresandxylemparenchyma Xylemfibresoftenaccompaniedbyvessels Stonecellsscatteredinphellodermandphloem cortex 栓皮 皮层phelloderm 栓内层 7 Reddish brown Stonecellsoccurringsinglyandscatteredthroughout subrounded subrectangular subfusiformorirregular upto257 mlong 20 65 mindiameter someofthemcontainingbrowncontents Reticulatedandborderedpittedvessels andbundlesofxylemfibresfrequent Corkcellsyellowish brown subsquareorpolygonalinsurfaceview containingreddish brownpigments dissolvedonmountinginchloralhydratesolution Powder surfaceview 表面观mount 做标本chloralhydrate 水合氯醛 8 Tanshinone tanshinone A tashinone B cryptotanshinone dihydroisotanshinone hydroxytanshinone isotanshinone iso cryptotanshinone miltirone salviol etc 化学成分 丹参酮 丹参酮 A 丹参酮 B 隐丹参酮 二氢丹参酮 羟基丹参酮 异丹参酮 异隐丹参酮 丹参新酮 鼠尾草酚等 ChemicalConstituents 9 tanshinone A丹参酮 A salvianolicacidB丹酚酸B 10 1 Boil5gofthepowderin50mlofwaterfor15 20minutes coolandfilter Concentratethefiltrateonawaterbath dissolvetheextractin3 5mlofethanol filter Applyseveraldropsofthefiltratetoapieceoffilterpaper allowittodryandexamineunderultra violetlight 365nm abrightbluish greyfluorescenceisproduced Exposethefilterpapertoammoniavapourfor20minutes removethefilterpaperandexamineagainunderultra violetlight 365nm apalebluish greenfluorescenceisproduced PhysicalandchemicalIdentification 11 理化鉴别 1 置5g粉末于50ml水中煎煮15 20分钟 冷却后过滤 在水浴中浓缩滤液 用3 5ml乙醇溶解浓缩产物 过滤 将滤液在滤纸上点一些斑点 斑点挥干后在365nm波长紫外光下观察 可看到明亮的蓝灰色荧光 将滤纸置于氨气中20分钟后 在365nm波长紫外光下观察 可见淡蓝绿色荧光 12 2 To0 5mlofthefiltrateobtainedfromIdentification 1 add1 2dropsofferricchlorideTS adullgreencolorisproduced ferricchloride 三氯化铁TS testsolutiondull 暗的 2 从 1 的滤液中移取0 5ml 加1 2滴三氯化铁试液 可见滤液变为暗绿色 13 3 To1gofthepowderadd5mlofether shakeinastopperedtesttubeandstandfor1hourandfilter Evaporatethefiltratetodryness Dissolvetheresiduein1mlofethylacetateasthetestsolution PrepareasolutionofRadixSalviaeMiltiorrhizaereferencedruginthesamemanner usedasthereferencedrugsolution DissolveTanshinone ACRSinethylacetatetoproduceasolutionof2mgpermlasthereferencesolution astopperedtesttube 具塞试管standfor 静置residue 残渣referencedrug 对照药材CRS ChemicalReferenceStandard 14 3 取1g粉末加5ml石油醚于有塞试管中振摇 静置1小时后过滤 将滤液蒸干 残渣用1ml乙酸乙酯溶解后作为供试品溶液 用同样方法制备丹参对照药材的溶液 作为对照药材溶液 将丹参酮 ACRS溶解在乙酸乙酯中制成每ml中含2g丹参酮 A溶液 作为对照品溶液 15 Carryoutthemethodforthinlayerchromatography usingsilicagelGasthecoatingsubstanceandbenzene ethylacetate 19 1 asthemobilephase Applyseparatelytotheplate5 lofeachofthethreesolutions Afterdevelopingandremovaloftheplate dryitinair 16 Thespotsinthechromatogramobtainedwiththetestsolutioncorrespondinpositionandcolourtothespotsinthechromatogramobtainedwiththereferencedrugsolution andthedark redspotinthechromatogramobtainedwiththetestsolutioncorrespondsinpositionandcolourtothespotinthechromatogramobtainedwiththereferencesolution correspondsto 对应于 与一致 17 照薄层层析色谱方法 用硅胶G作为固定相 苯 乙酸乙酯 19 1 作为展开剂 将3份溶液分别点5 l于同一薄层板上 展开 晾干 供试品溶液的色谱中在与对照药材溶液色谱的相应位置上有相同斑点 并且供试品溶液色谱中在与对照品溶液色谱相应位置上有相同的深红色斑点 18 Pharmacology 1 Coronaryartery dilatingeffect Itcanincreasebloodflowbydilatingcoronaryarteries 2 Sedativeeffect 3 Antibacterialandantifungaleffects ItstronglyinhibitsStaphylococcusaureus andhasadefiniteinhibitoryactiononMycobacteriumtuberculosisandsomedermatophytes 药理作用 1 扩张冠状动脉作用 通过扩张冠状动脉可增加血流量 2 镇静作用 3 抗菌 抗真菌作用 能强烈抑制金黄色葡萄球菌 并能明显抑制肺结核杆菌和一些皮肤真菌 19 Action Topromotebloodcirculationandstimulatemenstrualdischarge toremovebloodstasisandrelievepain andtonourishtheheartandtranquilizethemild 功效 活血调经 祛瘀止痛 养心安神 20 Indication Menstrualdisorders amenorrhea dysmenorrheal postpartumabdominalpainduetobloodstasis neurasthenia anginapectorisincoronaryheartdisease hepatosplenomegaly carbunclesanderysipelas 适应症 月经不调 闭经 痛经 产后血液停滞引起的腹痛 神经衰弱 冠心病心绞痛 肝脾大 痈和丹毒 21 照高效液相色谱法 附录VID 测定色谱条件及系统适用性试验以十八烷基硅烷键合硅胶为填充剂 以甲醇 水 75 25 为流动相 检测波长为270nm 理论塔板数按丹参酮 A峰计算应不低于200