邻苯二甲酸二丁酯雄生殖分子作用机制.doc

RT-PCR(DBP)(MIS)(ABP)(inhibin)ELISADBP DBPMISDBP(1000mg/kg)ABP(inhibin)mRNA- DBPABPinhibinDBP (DBP)()12DBP(phthalates)DBPF1(T)(Androgen-binding protein,ABP)(inhibin)(MIS)-1 11 DBP(995%)-80(Q/CYDZ-162-97)(Biotech)1(zona cocludens1)ZO1)(Zymed Laboratories)(TRITCIgG)()12 3DBP502001000mg/kg1(0mg/kg)100g DBP1280500ml12808013 131 SPFSprague-Dawley(-)152NO.02-4-20100(20020)g50(30030)g132 (223)50%60%12h12h133 1d(gestation day 1,GD1)21d(postnatal day 21,PND21)105ml/(100gbw)F1PND70d134 SD11240d(GD0)2202d(GD1)(PND21)4d(PND4)(OECD)(No.414)38(44)81614F11421d5-70RNA!-empirenews.page-14 ELISAPND70d(Biotech)15 RNA TRIzolRNA16 RTPCR GenebankSDRNAPrimer5ABPInhibinMISactin(470bp):Sequence 5CGGATGTCCACGTCA CACTT3,antisequence 5GTTGCTATCCAGGCTGTGCT3;ABP(286bp):Sequence 5ATCAACTGCTTGGGGTTCAC3,antisequence 5GAGCCTGGTCAGAGGCATAG3;Inhibin(208bp):Sequence 5GCTCTACCAGGGAGCATGAG3,antisequence 5CACCTTCCTCCTAGCTGACG3;MIS(307bp):Sequence 5AGCAAGGCTTCCCTGACGGT3,antisquence 5CAGGGTATAGCACTAACAGG3;GAPDH(153bp):Sequence 5GTGCTGAGTATGTCGTGGAG3,antisequence 5TGTCATATTTCTCGTGGTTC-317 RTPCR RTPCR1l3lPCRPCR5030min942min94 30s5564 30sactinABP56inhibinMIS(Glyceraldehyde3Phosphate Dehydrogenase,GAPDH)5872 60s,30357210min5l1l1530minDNA18 SPSS1102 21 PND1d F1MIS(11) 304bpsMISDBP22 PND70d F1 DBPF1DBPF1(P>005)23 PND14PND21d F1ABPinhibin(231) 1000mg/(kgbw)PND14PND21ABPinhibin50200mg/(kgbw)ABPPND21PND14inhibinPND14PND211 DBPABPinhibinMISa PCL50mg/kg DBPM200mg/kg DBPH1000mg/kg DBP1 DBPPND1d F1MISCL50mg/(kgbw) DBPM200mg/(kgbw) DBPH1000mg/(kgbw) DBP!-empirenews.page-2 DBPPND14dABPinhibinCL50mg/(kgbw) DBPM200mg/(kgbw) DBPH1000mg/(kgbw) DBP3 DBPPND21dABPinhibin3 DBP4-7DBP(MBP)(AR)ARDBP(12 )DBPT8-12DBPTTTABPinhibin-FSH1000mg(kgbw)DBPinhibinmRNADBPABPi