TaKaRa 蛋白质分子量标准(低).doc

o Protocolso Standard Protocolo Manual/DatasheetCat.#ProductSizeNote3450Protein Molecular Weight Marker (Low) 200 lanes3451Protein Molecular Weight Marker (High) 200 lanes3452Protein Molecular Weight Marker (Broad) 200 lanesDescriptionProtein Molecular Weight Markers are designed for use in SDS-Polyacrylamide gel electrophoresis. There are 3 kinds of Protein Molecular Weight Marker, Low (Molecular weight range : 14.3 - 97.2 kDa) , High (Molecular weight range : 44.3 - 200 kDa) and Broad (Molecular weight range : 6.5 - 200 kDa).Each protein is proportioned to yield uniform band intensities when perform staining Coomassie Brilliant Blue R-250 after run on SDS polyacrylamide gel.5l of 20-fold diluted marker is loaded per lane of SDS-PAGE minigel for standard use. In this case, this marker is sufficient for 200 lanes.Electrophoresis ResultLow(Cat.# 3450)High(Cat.# 3451)Broad(Cat.# 3452)15% SDS-PAGE7.5% SDS-PAGE5-20% gradientSDS-PAGEStorageProtein Molecular Weight Marker, 1 M DTT:-20C5Loading Buffer:Store at room temperature after used.ComponentProtein Molecular Weight Marker50 l5Loading Buffer1 ml1 M DTT100 lConcentration12 g/l (Cat.# 3450)10 g/l (Cat.# 3451)18 g/l (Cat.# 3452)Form50 mMTris-HCl, pH6.81 mMEDTA200 mMNaCl50%glycerolComponent ProteinLow(Cat.# 3450)ProteinSourceM.W.(Da)Phosphorylase BRabbit muscle97,200Serum AlbuminBovine66,409OvalbuminHen egg white44,287Carbonic anhydraseBovine29,000Trypsin InhibitorSoybean20,100LysozymeHen egg white14,300High(Cat.# 3451)ProteinSourceM.W.(Da)MyosinPig200,000-galactosidaseE. coli116,000Phosphorylase BRabbit muscle97,200Serum AlbuminBovine66,409OvalbuminHen egg white44,287Broad(Cat.# 3452) ProteinSourceM.W.(Da)MyosinPig200,000-galactosidaseE. coli116,000Phosphorylase BRabbit muscle97,200Serum AlbuminBovine66,409OvalbuminHen egg white44,287Carbonic anhydraseBovine29,000Trypsin InhibitorSoybean20,100LysozymeHen egg white14,300AprotininBovine pancreas6,5005Loading Buffer200 mMTris-HCl , pH6.810%SDS0.05%BPB50%GlycerolNote All products are intended to be used for research purpose only. They are not to be used for drug or diagnostic purposes, nor are they intended for human use. They shall not to be used products as food, cosmetics, or utensils, etc Takara products may not be resold or transferred, modified for resale or transfer, or used to manufacture commercial products without written approval from TAKARA BIO INC. If you require licenses for other use, please call at +81 77 543 7247 or contact from our website at Please confirm the content about the license or patent used in this document that relates to the Takara Bio product by clicking the license mark.Moreover, please confirm the Li