Nanog基因论文Nanog和Oct-4基因在前列腺癌中的表达及.doc

Nanog基因论文：Nanog和Oct-4基因在前列腺癌中的表达及意义【中文摘要】检测Nanog和Oct-4基因在良性前列腺增生组织,前列腺上皮内瘤变组织和前列腺癌组织中的表达,并研究Nanog和Oct-4的表达与在良、恶性前列腺增生性疾病中的意义,分析其与临床病理因素的相关性。方法：培养肿瘤细胞株(LNCaP.和PC-3),收集30例良性前列腺增生组织、8例前列腺上皮内瘤变组织,及94例前列腺癌组织。根据前列腺癌组织Gleason评分分为三组,即Gleason评分8分。运用免疫组织化学方法检测Nanog和Oct-4在不同肿瘤细胞株,良性前列腺增生组织、前列腺上皮内瘤变组织以及不同Gleason评分的前列腺癌组织中的表达,研究年龄、血清TPSA因素与两种基因的表达相关性。结果：1、前列腺癌细胞爬片免疫荧光染色显示,LNCaP和PC-3细胞株中Nanog基因在细胞核中较高表达,少量表达于胞浆；Oct-4基因主要在细胞核中表达,也可见少量胞浆表达。2、Nanog基因与Oct-4基因在良性前列腺增生中的表达率分别为16.7%(5/30)与10.0%(3/10)；这两种基因在前列腺上皮内瘤变组织的表达率分别为25%(2/8)与50%(4/8)；这两种基因在前列腺癌中的表达率分别为100%(94/94)与95.7%(90/94)。3、随着前列腺癌Gleason评分的增加,肿瘤中干细胞相关基因Nanog,Oct-4的表达也明显增多。这两种基因的表达与患者年龄、血清TPSA无明显关系。结论：Nanog,Oct-4基因在良性前列腺增生中偶有微量表达；这两种基因在前列腺上皮内瘤变中低量表达；这两种基因在前列腺癌中均有表达,其表达水平与肿瘤的组织学分级正相关。【英文摘要】s:To investigate the expression of Nanog and Oct-4 gene in BPH,PIN and PCa, analysis its effect on the development of the disease and its clinical significance.Methods:In this study, expression of Nanog and Oct-4 was detected by immunohistochemistry analysis in 132 patients,30 had BPH,8 had PIN,94 had prostate cancer (PCa). And between these PCa patients, there are 23 of them GleasonScore below 4,42 of them GS between 5to 7,and 29 of them GS higher than 8. Associations with various clinical pathological prognostic variables were analyzed in 94 PCa patients.Results:1. According to PC-3 and LNCaP Immunofluorescence test, there are obvious red fluorescence in both PC-3 and LNCaP cell nucleus, we also observe weak red fluorescence in a few prostatic cell cytoplasm.2. Expression levels of Nanog and Oct-4 were higher in PCa (100% and 95.7%) tissues than in PIN (25% and 50%) tissues; Expression levels of Nanog and Oct-4 in PIN tissues were slightly stronger than BPH (16.7% and 10.0%) tissues.3. There are positive relationship between Gleason Score and the expression of Nanog or Oct-4. Associations with various clinical pathological prognostic variables such as patientsage and TPSA seems no obvious relationship with the expression of Nanog or Oct-4.Conclusions:Nanog and Oct-4 expression levels showed significantly differences according to different PCa stage. Our findings indicate that Nanog and Oct-4 may be involved in carcinogenesis of the prostate and progression of PCa.【关键词】Nanog基因 Oct-4基因 前列腺癌【英文关键词】Nanog PCa Oct-4【目录】Nanog和Oct-4基因在前列腺癌中的表达及意义摘要3-4ABSTRACT4第1章 前言8-10第2章 材料与方法10-172.1 主要仪器和试剂10-112.2 实验步骤11-162.2.1 主要工作液的配制112.2.2 免疫组化检测BPH、PIN、PCa组织中Oct-4及Nanog基因的表达11-142.2.3 免疫荧光法检测前列腺癌PC-3和LNCaP细胞株中Nanog基因和Oct-4基因的表达14-162.3 统计学处理16-17第3章 结果17-253.1 Nanog基因和Oct-4基因在PC-3和LNCaP前列腺癌细胞株的表达173.2 免疫组化检测Nanog基因和Oct-4基因在前列腺组织中的表达17-253.2.1 Nanog基因和Oct-4基因在BPH、PIN和PCa中的表达率及其与年龄、血清TPSA的关系17-203.2.2 Nanog基因在BPH和PIN中的表达程度比较20-213.2.3 Nanog基因在BPH和PCa中的表达程度比较21-223.2.4 Oct-4基因在BPH和PIN中的表达程度比较223.2.5 Oct-4基因在BPH和PCa中的表达程度比较22-233.2.6 Nanog基因和Oct-4基因在不同分化的前列腺癌中的表达程度比较23-25第4章 讨论25-304.1 Nanog基因和Oct-4基因在干细胞中的作用25-274.1.1 Oct-4基因、Nanog基因与细胞重编程25-264.1.2 Oct-4基因、Nanog基因与外源信号的交互信号通路26-274.2 Nanog基因和Oct-4基因在肿瘤中的表达274.3 Nanog基因和Oct-4基因在良性前列腺增生、前列腺上皮内瘤变和前列腺癌中的表达27-304.3