人端粒酶反转录酶基因转染人胚胎大脑皮质神经元的凋亡.docx

人端粒酶反转录酶基因转染人胚胎大脑皮质神经元的凋亡吴灵芝1，李水彬1，成钢卫1，汪华侨2，孔令平31中山大学附属梅州医院神经内二科，广东省梅州市 514031；2中山大学中山医学院解剖学教研室脑研究室，广东省广州市 510080；3广州医科大学卫生职业技术学院，广东省广州市 510925Apoptosis of human embryonic cerebral cortex neurons transfected with human telomerase reverse transcriptaseWu Ling-zhi1, Li Shui-bin1, Cheng Gang-wei1, Wang Hua-qiao2, Kong Ling-ping31Second Department of Neurology, Meizhou Affiliated Hospital of Sun Yat-sen University, Guangzhou 514031, Guangdong Province, China; 2Department of Anatomy and Brain Research, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China; 3School of Health Vocational Technology, Guangzhou Medical University, Guangzhou 510925, Guangdong Province, China摘要 背景：人端粒酶反转录酶重组腺病毒转染原代培养的人胚胎大脑皮质神经元，可以促进细胞生存，抑制凋亡。目的：观察人端粒酶反转录酶对淀粉样蛋白(amyloid -protein，A)诱导人胚胎大脑皮质神经元凋亡的影响。方法：人胚胎大脑皮质神经元原代培养后，分为3组：对照组、A25-35组和人端粒酶反转录酶组。A25-35组和人端粒酶反转录酶组细胞在培养144 h后，用A25-35 5 mol/L干预24 h。人端粒酶反转录酶组在A25-35 5 mol/L干预72 h进行人端粒酶反转录酶基因转染。结果与结论：原代培养的人胚胎大脑皮质神经元培养7 d后，出现凋亡细胞，而A25-35干预后使凋亡细胞数量增加，而人端粒酶反转录酶可防止A25-35诱导的人胚胎大脑皮质神经元的凋亡。中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程全文链接：窗体顶端关键词 ： 组织构建； 组织工程； 干细胞； 胚胎干细胞； 人端粒酶催化亚基； 诱导； 人胚胎大脑皮质神经元； 阿尔茨海默病； 凋亡； 端粒酶； 反转录酶； 转染； Abstract： BACKGROUND: Human telomerase reverse transcriptase recombinant adenovirus transfection could promote the survival and inhibit the apoptosis of primary cultured human embryonic cerebral cortex neurons.OBJECTIVE: To observe the effects of human telomerase reverse transcriptase on the apoptosis of human embryonic cerebral cortex neurons induced by amyloid -protein invention.METHODS: Primary cultured cerebral cortex neurons of human embryo were divided into three groups, namely control group, amyloid -protein (25-35) group, and human telomerase reverse transcriptase group. Except the control group, the cells were cultured for 144 hours and intervened with 5 mol/L amyloid -protein (25-35) for 24 hours. Furthermore, the cells in human telomerase reverse transcriptase group were transfected with human telomerase reverse transcriptase group at 72 hours after intervention of 5 mol/L amyloid -protein (25-35).RESULTS AND CONCLUSION: After human embryonic cerebral cortex neurons were cultured for 7 days, the apoptotic cells were found in three groups. The apoptosis of neurons was obviously increased after intervention of amyloid -protein (25-35). Human telomerase reverse transcriptase transfection could prevent the apoptosis of human embryonic cerebral cortex neurons induced by amyloid -protein (25-35).中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程全文链接：Key words： cerebral cortex; apoptosis; telomerase; telomerase-binding proteins; transfection; 窗体底端 中图分类号:R318 基金资助: 广东省教育厅科技创新项目(2012KJCX0089)通讯作者: 孔令平，博士，副教授，广州医科大学卫生职业技术学院，广东省广州市 510925 作者简介: 吴灵芝，女，1981年生，山东省滨州市人，汉族，硕士，主治医师，主要从事中枢神经退行性病变基础方面的研究。 引用本文: 吴灵芝,李水彬,成钢卫等. 人端粒酶反转录酶基因转染人胚胎大脑皮质神经元的凋亡J. 中国组织工程研究, 2014, 18(29): 4653-4657. Wu Ling-zhi,Li Shui-bin,Cheng Gang-wei et al. Apoptosis of human embryoni