小单孢菌属及拟诺卡氏菌属.ppt

稀有放线菌的分离与培养 报告人 张晓敏 报告内容 分离培养基的选择小单孢菌形态与生理生化特征拟诺卡氏菌形态与生理生化特征小单孢菌 拟诺卡氏菌与链霉菌区别 分离培养基的选择 淀粉 酪蛋白硝酸盐琼脂培养基溶胶几丁质琼脂培养基甘露醇 利福平琼脂培养基 淀粉 酪蛋白硝酸盐琼脂培养基 starch caseinnitrateagar starch10g sodiumcaseinate0 3g KNO32g agar15g PH7 0 7 4 37 gnovobiocin 223 gmycostatin30 培养21天Micromonosporachaiyaphumensissp novMicromonosporaeburneasp nov 基础培养基 K2HPO40 7g KH2PO40 3g MgSO4 7H2O0 5g FeSO4 7H2O0 01g ZnSO40 001g 琼脂10克 蒸馏水1 0L PH7 0121 蒸汽灭菌20min 每100mL加20mL胶体几丁质悬浮液和5mg放线菌酮 溶胶几丁质琼脂培养基 甘露醇 利福平琼脂培养基 mannitol rifampicinagar 甘露醇10g 酵母提取物7g 酪蛋白水解物2g 蛋白胨1g NaCl1g CaCO30 2g 制霉菌素100mg 利福平5mg27 培养6周Nocardiaacidivoran 返回 Micromonospora gyrB基因序列分析和DNA DNA杂交15species Micromonosporaaurantiaca Micromonosporacarbonacea Micromonosporachalcea M chersina Micromonosporacoerulea Micromonosporaechinospora Micromonosporagallica Micromonosporahalophytica Micromonosporainositola M matsumotoense Micromonosporanigra M olivasterospora Micromonosporapallida MicromonosporapurpureochromogenesandM rosaria 小单孢菌形态与生理生化特征 Aerobic Gram positive mesophilic non motileactinomycetewell developedandbranchedsubstratehyphae Aerialmyceliumwasabsent Sporechainswereabsent Mycolicacidswereabsent Acyltypeofcellwallmuramicacidisglycolyl Fatty acidtype 3b Thepredominantmenaquinone MK 9 H4 MK 9 H4H6H8 Characteristicwhole cellsugarsarexyloseandarabinose Phospholipids P II型 Cell wallpeptidoglycan CW II glutamicacid alanineandmeso diaminopimelicacid DNAG C mol 71 73 well developedandbranchedsubstratehyphae Aerialmyceliumwasabsent Atmaturity sporesweresphericaltooval appearedtoberoughandwerenon motile Fig 1 colourofthevegetativemyceliumonISP2mediumisorange turningtogreyishblackorblackuponsporulation growthatpH4 8and25 30 nogrowthabove45 Brown diffusiblepigment positivefortheutilizationofL arabinose D fructose D galactose D glucose lactose melibiose salicinandD xylose negativefornitratereductionandtheutilizationofD mannitolandrhamnose Micromonosporachaiyaphumensis Single non motile sphericalspores 0 6 0 9mm wereformedonsubstratehyphae Aerialmyceliumisabsent Thesporesurfaceappearswarty Sporesarenon motile Thecolourofthespulatingcolonieswasyellowishwhitetopaleorange Ayellow solublepigment UtilizesL arabinose D fructose D galactose D glucose lactose D ribose salicinandD xylose weakutilizationofcellobiose notutilizeglycerol D mannitol melibiose raffinoseorL rhamnose Micromonosporapattaloongensis well developedandbranchedsubstratehyphae Single non motilesporesareformedonsubstratehyphae 0 45mmindiameter Aerialmyceliumisabsent Thespore surfaceappearsroughandnodular Coloniesareyellowishwhiteandturngreyishblackaftersporulation Paleyellowsolublepigment positivefornitratereduction UtilizesD glucose L rhamnose D melibiose D raf nose glycerol salicin lactose cellobioseandD xylose weaklyutilizesD mannitolandD galactosebutnotL arabinose D fructoseorD ribose Micromonosporaeburnea 生理生化区别 返回 拟诺卡氏菌属 原拟诺卡氏菌属包括7个种2个亚种 Grund等根据化学分类和数值分类研究