Strukturbiochemie中子散射.ppt

PDDr RegineWillumeitGKSSForschungszentrumGeesthachtMax Planck Str 121502GeesthachtTel 04152871291Mobil 01704316430Email willumeit gkss de StrukturbiochemieI Donnerstag 17 00 19 00Uhr10 05 200724 05 200707 06 200721 06 200705 07 2007 ExkursionzurGKSS Termine Neutronen undR ntgenstreuung gel steSysteme 3hFluoreszenzspektroskopie verschiedeneVarianten 2hSurfacePlasmonResonance 1hInfrarot undCD Spektroskopie 2hMassenspektroskopie 1hZetaPotential 1h ThemenderVorlesung AllgemeineVorbemerkungen Strukturbiochemie wasistdas ErforschtdieStrukturvonProteinen Nukleins uren Lipiden Zucker Komplexen Ribosomen Membranen Zellen ErforschtdieFunktionundDynamikvonMolek lenUntersuchtdieWechselwirkungvonMolek len Protein strukturanalyse DiewichtigstenDatenbanken http www rcsb org pdb home home do EM NMR http www ncbi nlm nih gov BLAST http ndbserver rutgers edu NDB NDBATLAS URLfunktioniertgerademalnicht Literatur Google Wikipedia Grundlagen WechselwirkungmitMaterieKontrastvariationAufbaueinesInstrumentesDatenaufnahmeundAuswertungStrukturanalyseanBeispielen Neutronen undR ntgenstreuung gel steSysteme AccessableSizeRegimes IntroductiontoScattering electromagneticwaves electromagneticwaves shortwavelength particles chargedparticles OnlyElasticScattering Inelasticscattering Transferofenergy momentuminto fromthesampleatoms InteractionofRadiationwithMatter InteractionofRadiationwithMatter Interactionwithelectrons Interactionwithelectrons Scattering strength isproportionaltoZ Interactionwithelectronspinpossible Interactionwithnuclei protonsandneutrons Scattering strength doesnotvarysystematically Interactionwithnuclearspinpossible Interactionwithelectronsandelectronspinpossible AtomicScatteringfactors length H R Winter F Noll MethodenderbiophysikalischenChemie Teubner 1998 X Rays Neutrons atomicmass gmol 1 ComparisonNeutron andX ray scatteringlengthsomerelevantelements 10 12cm nX ray1H 0 370 282H0 670 2812C0 661 6814N0 941 9616O0 582 2431P0 514 232S0 284 4856Fe0 956 72 NeutronScatteringLengthofbiologicalrelevantelements 10 12cm F Sears 1986 H Gl ttliundM Goldmann 1987 X RaySpeciality AnomalousScattering ASAXS NeutronsX RaysIntensitylowhighH sensitivityhighnoneIsotope sensitivitystrongnoneHeavyelementslowhighSpin sensitivitystrongaveragePenetrationdepthhighlowSamplesize amountlargesmallMeasurementtimelongshortInteractionwithnucleielectronshellelectronshellunsystematicaZRadiationdamagenonehigh ToRemember ToRemember H Zabel Bochum ContrastVariation Whenthemonstercame Lola likethepepperedmothandthearctichare remainedmotionlessandundetected Haroldofcourse wasimmediatelydevoured TheConceptofContrastVariation Contrast DifferenceofScatteringLengthDensities Contrast DifferenceofScatteringLengthDensities p R rParticle R rLM R p R rParticle R rLM r R ScatteringLengthDensitiy SumofScatteringLengthofallAtomsinaVolume TheConceptofContrastVariation Contrast DifferenceofScatteringLengthDensities ChangeofsolventcompositionChangeofsamplecomposition IsotopicsubstitutionChangeofscatteringprocess Usually Combinationofthem Examples Polymer SurfactantInteraction JuhaMertaandPerStenius HelsinkiUniversityofTechnology Espoo FinlandVasilM Haramus Materials SyntheticCationicPolymer Poly diallyldimethylammoniumchloride PDADMACM 5 50 104 30 104 AnionicSurfactant Sodiumhexadecanoate NaPal C15H31COONa CAC 0 1mM CMC 1 6mM Kraftpoint 62oC Application Howarethetwocomponentsorganized InternalContrastVariation C15H31COONa NaPal H C15D31COONa NaPal D SolventD2O WhereisthesurfactantNaPalandwherethepolymerPDADMAC Examples Polymer SurfactantInteraction H D PDADMAC NaPal D2O Examples Polymer SurfactantInteraction InternalContrastVariation Examples Polymer SurfactantInteraction Conclusion ShellStructureofPDADMAC NaPal SurfactantCore PolymereShell J Merta V M Garamus R Willumeit P Stenius 2002 Langmuir 18 7272 7278 DistanceDistribution Compositionofbacterialribosomes R J nemannundK H Nierhaus NaturwissenschaftlicheRundschau10 1993 391 400 D I Svergunetal JMB271 1997 602 618 RibosomeStructures D I Svergunetal JMB271 1997 602 618 RibosomeStructures D I Svergunetal JMB271 1997 602 618 ShapeandRNA distribution Modelling RibosomeStructures NeutronScatteringLengthofbiologicalrelevantelements 10 12cm F Sears 1986 H Gl ttliundM Goldmann 1987 SpinContrastVariation deuterated isotopicsubstitution InsituStructuresof14