葡萄糖标准曲线.doc

2013/3/ 15:24 /Graph1 (2456363)Linear Regression through origin for Data1_B:Y = B * XParameterValueError-A0-B0.802030.01276-RSDNP-0.99820.0364690.0001-氮源种类lsd=t0.05*sx1-x2 sx1-x2=N=3 L0.05=45.877 L0.01=65.25334氮源用量L0.05=57.9393 L0.01=82.4102硫酸铵和豆饼粉含量L0.05=36.0044 L0.01=51.211硫酸铵和蛋白胨含量L0.05=38.3182 L0.01= 54.50201-葡萄糖苷酶CxC111结晶纤维素无定形纤维素纤维二糖葡萄糖纤维二糖EGI、EG II纤维糊精CBHI、CBH IICBHI、CBH II 纤维素 BG葡萄糖实验号因素酶活值（U/g）ABCD平均值11111276.502259.698291.778275.99221222301.122251.622348.146300.29731333267.357253.553244.109255.00642123238.445248.778294.082260.43552231332.598291.779302.362308.91362312288.606398.204405.511364.10773132377.461383.599329.895363.65283213444.975436.250438.841440.02293321477.430446.196423.886449.171K1831.296900.0811080.1220T=3017.599K2933.4561049.2331009.9040K31252.8461068.285927.5720277.098300.026360.0410311.152349.744336.6340417.615356.095309.1910R140.51656.06850.8490（2）正交实验结果表16 正交实验结果及分析Table16. Results and analysis of orthogonal test实验号因素酶活值（U/g）ABCD平均值11111276.502259.698291.778275.992 21222301.122251.622348.146300.29731333267.357253.553244.109255.00642123238.445248.778294.082260.43552231332.598291.779302.362308.91362312288.606398.204405.511364.10773132377.461383.599329.895363.65283213444.975436.250438.841440.02293321477.430446.196423.886449.171 K1831.296900.0811080.1220T=3017.599 K2933.4561049.2331009.9040K31252.8461068.285927.5720 277.098300.026360.0410 311.152349.744336.6340 417.615356.095309.1910 R140.51656.06850.8490本项目以绿色木霉为菌种，对绿色木霉产纤维素酶固态发酵培养基配方中氮源进行选择优化，提高产纤维素酶的活力。采用单因素实验分别研究了培养基生长氮源种类、生长氮源用量对产纤维素酶活力的影响，再通过研究培养基中有机氮源和无机氮源的不同组合对产纤维素酶活力的影响，最后通过正交实验三种不同氮源组合对培养基配方中氮源进行了优化，以确定最优氮源组合。实验结果表明培养基配方中最优氮源组合为：硫酸铵为1.2%，豆饼粉为0.4%，蛋白胨为0.4%。采用此培养基中最优氮源组合固态发酵培养绿色木霉产纤维素酶活力达到448.558U/g。The project is with green trichoderma viride strain, for green trichoderma viride produces cellulase nitrogen source in solid state fermentation culture medium formula selection optimization and enhance the vitality of cellulase production. Culture medium was studied by single factor experiment respectively growth nitrogen source type, dosage of nitrogen source on the influence of the cellulose enzyme activity, through the medium of the different combination of organic nitrogen source and inorganic nitrogen source to produce cellulose enzyme activity influence, finally through the orthogonal experiment of three different nitrogen source combination of nitrogen source in the culture medium formula has been optimized, in order to determine the optimum nitrogen source. Experiments showed that the optimal nitrogen source in the culture medium formula combination are: ammonium sulfate is 1.2%, bean cake powder is 0.4%, peptone 0.4%. Using the medium combination of the optimal nitrogen source in solid state fermentation culture green trichoderma viride produces cellulose enzyme activity reached 448.558 U/g.With the green trichoderma viride producing cellulose, the project selectes optimized nitrogen source of culture medium to enhance the vitality of cellulase production. Growth nitrogen source type and dosage of nitrogen source in culture medium is studied by experiment of single factor the influence of the cellulose enzyme activity,then the different combination of organic nitrogen source and inorganic nitrogen source of the culture medium is studied the influence of the cellulose enzyme activity which is produced by the green trichoderma viride. Finally,the optimized combination of organic nitrogen source and inorganic nitrogen source to produce the higher cellulose enzyme activity has been selected. Experiments showed that the optimal nitrogen source in the culture medium formula combination are: ammonium sulfate is 1.2%, bean cake powder is 0.4%, peptone 0.4%. The green trichoderma viride produces cellulose enzyme activity reached 448.558 U/g by using the combination of the optimal nitrogen source in solid fermentation culture medium.本项目以绿色木霉为菌种，对绿色木霉产纤维素酶固态发酵培养基中氮源进行选择优化，以提高纤维素酶活力。采用单因素实验对培养基中氮源种类、氮源浓度、两种氮源配比组合进行了选择，再运用正交实验对三种氮源配比组合进行了优化。实验结果表明培养基中最优氮源组合为硫酸铵1.2%、豆饼粉0.4%、蛋白胨0.4%。采用此最优氮源组合的培养基进行固态发酵培养绿色木霉产纤维素酶活力达到448.558U/g。This project is with green trichoderma viride strain, for green trichoderma viride produces cellulase choose nitrogen source in solid state fermentation medium optimization, in order to improve the cellulose enzyme activity. Using single factor experiment to medium kind of nitrogen source and nitrogen source concentration, ratio of two kind of nitrogen source combination selection, again with the orthogonal experiment of three nitrogen source matching combination is optimized. Experiments showed that the optimal nitrogen source in the culture medium composition for ammonium sulphate 1.2% 0.4%, peptone 0.4%, bean cake powder. Adopt the combination of the optimal nitrogen source of solid state fermentation culture medium for cultivating green trichoderma viride produces cellulose enzyme activity reached 448.558 U/g.With the green trichoderma viride producing cellulose, the project selectes optimized nitrogen source of culture medium to enhance the vitality of cellulase production. Nitrogen source type and nitrogen source concentration, ratio of two kind of nitrogen source combination were selected by using single factor experiment, then with the orthogonal experiment of three nitrogen source matching combination was optimized. The result of experiment showed that the optimal n