人工血管英文王维慈

Histocompatbility of Polyurethane-silk fibroin composite with controlled release of aspirin,王维慈 Weici Wang欧阳晨曦 Chenxi Ouyang协和医院血管外科Division of vascular surgery，Union Hospital,National Natural Science Foundation of China国家自然科学基金“小口径人造血管的仿生构建与性能研究”,1,973 Pre-study Program973计划前期研究专项“非水溶性丝素粉体与功能聚合物复合小口径人造血管的结构与性能研究”,2,Back ground,Embolization,Atherosclerosis,Arterial Injury,IschemicNecrosis,Back ground,Revascularization,Vascular replacement,Bypass surgery,Human vesselsMan-made graft ,Back ground,Dacron,Great vessels（10mm）,Expanded polytetrafluoroethylene，e-PTFE,Small vessels（6mm）,Mid size(610mm),Own vessels ？,Back ground,Ideal small diameter graft,Back ground,Poly-urethane(PU),Dacron,Silk,Modified Method,Tubular Fabric Reinforced,Pre-studies,3 Tier Sturcture（endometrial，middle，outer）,Pre-studies,SF-PU(1:1) Graft,Biocompatibility& Mechanical properties,Drug release system,Mechanical properties,SF：Biocompatibility，Drug adsorption and release,PU：Biocompatibility，Mechanical properties,Tubular Fabric Reinforced3 Tier Sturcture,Silk Fibroin Powder Modified,Pre-studies,IV. Low temperature plasma technology sulfonatedSF Anti-clotting ability,Pre-studies,Drug releaseHistocompatbility of Polyurethane-silk fibroin composite with controlled release of aspirin,Present studies,Histocompatbility of Polyurethane-silk fibroin composite with controlled release of aspirin,Drug releasedSF-PU(1:1),Dog carotid arteryBy pass,ATR-FTIR,Blend film structure,Hematological Examination,1w Histological EX,4w Histological EX,Drug release,Conclusion,Histocompatbility of Polyurethane-silk fibroin composite with controlled release of aspirin,Blend film structure,Pure PU,10%- SF-PU cross-section,5%-SF-PU,0%-SF-PU,ATR-FTIR of Aspirin-SF-PU (1:1) blend film,Histocompatbility of Polyurethane-silk fibroin composite with controlled release of aspirin,Aspirin release study,37 ，relationship of aspirin release and time (means.d.,n=3),1st phase:Inhibition of thrombosis after surgery2nd phase:Reduce the riskof thrombosis,Histocompatbility of Polyurethane-silk fibroin composite with controlled release of aspirin,1w Histological Examination,Control e-PTFE 0%-SF-PU 5%-SF-PU 10%-SF-PU,（A： Completely healed ；B：Materials implanted）,Histocompatbility of Polyurethane-silk fibroin composite with controlled release of aspirin,1w Histological Examination (Tissue sections),5%-SF-PU,10%-SF-PU,0%-SF-PU,e-PTFE,A：materialB1：Pseudo-membranousB2：borderC：Inflammatory cellsD：Aspirin Crystals,Histocompatbility of Polyurethane-silk fibroin composite with controlled release of aspirin,1w Histological Examination （SEM & TEM）,A:Inflammatory cells;B:loose structure of e-PTFE, no cells,Histocompatbility of Polyurethane-silk fibroin composite with controlled release of aspirin,Hematological ExaminationWhite blood cell (WBC),WBC counts of rat with different biomaterials implanted after 1 week and 4 weeks (mean s.d., n=3).(,Histocompatbility of Polyurethane-silk fibroin composite with controlled release of aspirin,Hematological ExaminationBlood platelet (PLT),PLT counts of rat with different biomaterials implanted in after 1 week and 4 weeks (mean s.d., *p0.05 vs.control, n=3).,Histocompatbility of Polyurethane-silk fibroin composite with controlled release of aspirin,ConclusionTraditional e-PTFE: WORST5%Aspirin-SF-PU，BETTER 10%Aspirin-SF-PU，Myelosuppression?Aspirin release in SF-PU(1:1): Phase 1 and Phase 2,返 回,Histocompatbility of Polyurethane-silk fibroin composite with controlled release of aspirin,Dog carotid artery-By pass,实验分组Dog0：DacronDog1：SF-PU (1:1)Dog2：5%Aspirin-SF-PU (1:1),Dog carotid artery-By pass,Dog1、2：Flexible textureNo bleeding,Dacron：Hard and bleeding,Dog carotid artery-By pass,4 months,Dog carotid artery-By pass,After 4 months，MRA for 0,1 Dogs,Dog0:DacronDog1:SF-PUA：graftB : occluded,Dog carotid artery-By pass,Long-term survivalDog 0：6 months，abnormal head movement； 14 months， diarrhea and dead.Dog 1、2：18 months， alive,Dog carotid artery-By pass,18 months， remove grafts,Structure keepedmechanical characterNo thrombus,Dog carotid artery-By pass,18 months， remove graftsDog 0 proximal end of Dacron graft, 8cm thrombus,Dog carotid artery-By pass,18 months， remove grafts,10%Heparin-5%Aspirin-SF-PU (1:1) smooth and no thrombus ，But Anastomotic stenosis,返 回,Conclusion,Pre-study,Pre-study,present,5%Aspirin-SF-PU: best histo-compatibility, drug release system.Doc carotid artery by pass: Long-t