淡水、海水培养基配方

SE Medium Working solution g l NaNO30 25g K2HPO4 3H2O0 075g MgSO4 7H2O0 075g CaCl2 2H2O0 025g KH2PO40 175g NaCl 0 025 Soil extract 40ml FeCl3 6H2O0 005 Fe EDTA1ml A5 solution 1 distilled water958 Soil Extract 土壤提取液 配置方法 取花园土未施过肥 0 5kg 置于烧杯或三角瓶中 加入蒸馏水 1000 毫升 瓶口用透气塞封口 在水浴中沸水加热 2 小时 冷却数小时 在无菌条件下过滤 取上清液 将灭菌蒸馏水加入上 清液至总体积 1000 毫升 土壤提取液保存在 4 C 备用 Composition of the A5 solution Add to 100 ml of distilled water H3BO3286 mg MnCl2 4H2O181 mg ZnSO4 7H2O22 mg CuSO4 5 H2O7 9 mg NH4 6Mo7O24 4H2O3 9 mg EDTA Fe 的配置方法 将 EDTA 和 FeCl3 6H2O 分别溶于水和 HCl 0 1N 混匀即可 Na2EDTA1g distilled water50ml FeCl3 6H2O81 mg HCl 0 1N 50ml f 2f 2 MediumMedium Guillard Ryther 1962 Guillard 1975 To 950 mL filtered seawater add QuantityQuantityCompoundCompound StockStock SolutionSolutionMolarMolar ConcentrationConcentration inin FinalFinal MediumMedium 1 mL NaNO3 75 g L dH2O 8 83 x 10 4 M 1 mL NaH2PO4 H2O 5 g L dH2O3 63 x 10 5 M 1 mL Na2SiO3 9H2O 30 g L dH2O 1 07 x 10 4 M 1 mL f 2 trace metal solution see recipe below 0 5 mLf 2 vitamin solution see recipe below Make final volume up to 1 L with filtered seawater and autoclave Note Note Autoclaved f 2 medium produces extensive silica precipitate We delete silicate when it is not required by the alga see f 2 Si medium below f 2f 2 TraceTrace MetalMetal SolutionSolution Guillard Ryther 1962 Guillard 1975 To 950 mL dH2O add QuantityQuantityCompoundCompound StockStock SolutionSolutionMolarMolar ConcentrationConcentration inin FinalFinal MediumMedium 3 15 g FeCl3 6H2O 1 x 10 5 M 4 36 g Na2EDTA 2H2O 1 x 10 5 M 1 mL CuSO4 5H2O 9 8 g L dH2O4 x 10 8 M 1 mL Na2MoO4 2H2O 6 3 g L dH2O 3 x 10 8 M 1 mL ZnSO4 7H2O 22 0 g L dH2O 8 x 10 8 M 1 mL CoCl2 6H2O 10 0 g L dH2O 5 x 10 8 M