常见的通用引物序列.doc

奥科公司测序部通用引物合成引物名称引物序列T7TAATACGACTCACTATAGGGSP65ATTTAGGTGACACTATAG3M13F5TGTAAAACGACGGCCAGT3M13R5CAGGAAACAGCTATGACC3T7（17BASE）5ACATCCACTTTGCCTTTCTC3T7TER5TGCTAGTTATTGCTCAGCGG3S.TAG5CGAACGCCAGCACATGGACA3T35ATTAACCCTCACTAAAGGGA3M13F（47）5CGCCAGGGTTTTCCCAGTCACGAC3M13R（48）5AGCGGATAACAATTTCACACAGGA3PGEX55GGGCTGGCAAGCCACGTTTGGTG3PGEX35CCGGGAGCTGCATGTGTCAGAGG3PEGFPC55CATGGTCCTGCTGGAGTTCGTG3PEGFPC35TATGGCTGATTATGATCAGT3PEGFPN55TGGGAGGTCTATATAAGCAGAG3PEGFPN35CGTCGCCGTCCAGCTCGACCAG3PBV220F5AAGAAGGGCAGCATTCAAAG3PBV220R5CTG CGT TCT GAT TTA ATC TG35AOX5GACTGGTTCCAATTGACAAGC33AOX5GGCAAATGGCATTCTGACAT3AFACTOR5TAC TAT TGC CAG CAT TGC TGC3M13(96)5CCCTCATAGTTAGCGTAACG3PCMVF-BD5TCTAAAAGCTGCGGAATTGT3PCMVR5TCCAAACTCATCAATGTATC3P171105TACCACTACAATGGATG3P125845TTTTCAGTATCTACGAT3PAS21F5 TCATCGGAAGCGAGTAG3PAS21R5 CGTTTTAAAACCTAAGAGTCAC33AD5 AGA TGG TGC ACG ATG CAC AG33BD5TAA GAG TCA CTT TAA AAT TTG TAT C3PQE30F5 TGA GCG GAT AAC AAT TTC AC3PQE30R5 GTT CTG AGG TCA TTA CTG G3S15CAA CGT GAA AAA ATT ATT ATT CGC3S65GTA AAT GAA TTT TCT GTA TGA GG3GLP15TGT ATC TTA TGG TAC TGT AAC TG3GLP25 CTT TAT GTT TTT GGC GTC TTC CA3RVP35 CTA GCA AAA TAG GCT GTC CC3RVP45GAC GAT AGT CAT GCC CCG CG3PTRC99CF5TTG CGC CGA CAT CAT AAC3PTRC99CR5CTG CGT TCT GAT TTA ATC TG3PIRES2EGFPF5GTA GGC GTG TAC GGT GGG AG3PIRES3EGFPR5AAC GCA CAC CGG CCT TAT TC3PCDNA3.0F25CTC TGG CTA ACT AGA GAA CC3PCDNA3.0R25GGC AAC TAG AAG GCA CAG TC3BAC15AAC CAT CTC GCA AAT AAA TA 3BAC25ACG CAC AGA ATC TAG CGC TT 3PB42ADF5CCAGCCTCTTGCTGAGTGGAGATG3PB42ADR5AAGCCGACAACCTTGATTGGAG3TRXF5CATATGAGCGATAAAATTATTCAC3TRXR5GGATCCCTTGTCATCGTCATCACCA3pTARGETSEQ5TTA CGC CAA GTT ATT TAG GTG ACA3pMALC2XF5TGC GTA CTG CGG TGA TCA AC3pMALC2XR5CTG CAA GGC GAT TAA GTT GG3pbdgal4f5GCCTCTAACATTGAGACAGC3pbdgal4r5AAGAGTTACTCAAGAACAAGAA3pbi121(35s)5GACGCACAATCCCACTATCC3PEFF5 TCA AGC CTC AGA CAG TGG TTC3PCEPF5 AGA GCT CGT TTA GTG AAC CG3EBVR5 GTG GTT TGT CCA AAC TCA TC3CMVF5CGCAAATGGGCGGTAGGCGTG3CMVR5GTTCACGGTGCCCTCC3PTRCHISAF5CAT GGT ATG GCT AGC ATG AC3PTRCHISAR5CAG GCT GAA AAT CTT CTC TC3pDC316 F5 -TAA CTT GTT TAT TGC AGC -3pDC316 A5 -TAT GCT TAC CGT AAC TTG -3BGH5TAG AAG GCA CAG TCG AGG3pDC316F5 acg tgg gta taa gag gcg 3pDC316R5 cga tgc tag acg atc cag 3pshuttle-cmv F5-ggT CTA TAT AAg CAg AgC Tg-3pshuttle-cmv R5-gTg gTA Tgg CTg ATT ATg ATC Ag-3GAL4BD5TCA TCG GAA GAG AGT AG 3TADH5GTA AAT TTC TGG CAA GGT AGA C 3pDsRed-n5GACTTTCCAAAATGTCGTAAT7-EEV5AAG GCT AGA GTA CTT AAT ACG A 3pfastBac-5GTC CGA AAC CAT GTC GTA CpfastBac-3CTG ATT ATG ATC CTC TAG TAC TTCPBAD5TAT CGC AAC TCT CTA CTG TTPBAD35 AGC CAA GCT GGA GAC CGT TT 35ADAAT ACC ACT ACA ATG GAT GAT GN-CMV-30ATA ACC CCG CCC CGT TGC-CMV-24TTA GGA CAA GGC TGG TGGpBABE_5ctt tat cca gcc ctc acpBABE_3accctaactgacacacattccP5TGC GTA CTG CGG TGA TCA ACP3CTG CAA GGC GAT TAA GTT GGDuetDOWN15-GATTATGCGGCCGTGTACAA-3PET UpstreamATG CGT CCG GCG TAG AGG AT