内切酶活性表.doc

EnzymeSupplied NEBuffer% Activity in NEBuffers1234Aat II405050100Acc I4505010100Acc65 I3 + BSA107510025Aci I3255010050Acl I4 + BSA10100100Afe I *SE-Y255025100Afl II2 + BSA5010025100Afl III3 + BSA257510050Age I1100501075Ahd I4 + BSA25750100Ale I4102010100Alu I210010075100Alw I45010010100AlwN I45010050100Apa I 25C4 + BSA25500100ApaL I4 + BSA10010010100Apo I 50C3 + BSA107510075Asc I401010100Ase I3NR75dd100NRAsiS I3 + BSA5010010050Ava I4107510100Ava II4507510100Avr II210010050100Bae I 25C2 + BSA + SAM501005075BamH IUdd + BSA751005075Ban I45010050100Ban II41001050100Bbs I21001002575Bbv I2 1001002575BbvC I45010010100BceA IUdd + BSA25NRNR75Bcg IU + SAM507510050BciV I41002510100Bcl I 50C350100dd10075Bfa I4755010100BfrB I4 + BSA102510100BfuA I 50C307510010BfuC I4 + BSA1005025100Bgl I3507510050Bgl II3107510010Blp I45010010100Bme1580 I2751007575BmgB I3 + BSA02510010Bmr I27510075100Bmt I *SE-W251001050Bpm I3 + BSA75100100100Bpu10 I*SE-K02510025BpuE I 25C2 + SAM751001075Bsa I 50C37575100100BsaA I3757510075BsaB I 60C275100100100BsaH I4 + BSA50100100100BsaJ I 60C2100100100100BsaW I 60C2 + BSA501005050BsaX I475 100 10 100 BseR I210010075100BseY I3105010050Bsg I4 + SAM507550100BsiE I 60C2 + BSA5010010100BsiHKA I 65C3 + BSA50100100100BsiW I 55C310010010025Bsl I 55C3105010075Bsm I 65C2751007575BsmA I 55C35010010050BsmB I 55C375100100100BsmF I 65C4 + BSA255050100BsoB I 65C21010010050Bsp1286 I4 + BSA252525100BspCN I 25C1 + BSA + SAM100751075BspD I4257550100BspE I30101000BspH I41010050100BspM I3NRNR100NRBsr I 65C305010010BsrB I250100100100BsrD I 60C2 + BSA501005075BsrF IU251007575BsrG I2 + BSA2510010100BssH II 50C3100100dd100100BssK I 60C3 + BSA05010050BssS I3050dd10010BstAP I * 60CSE-W252510025BstB I 65C4755025100BstE II 60C3507510075BstF5 I * 65CSE-Y755025100BstN I 60C2 + BSA1010010075BstU I 60C210010050100BstX I 55C32510010050BstY I 60C25010075100BstZ17 I3NRNR100NRBsu36 I3 + BSA0251000Btg I3 + BSA2550100100Bts II4 + BSA 100 50 50 100 Cac8 I3507510075Cla I4 + BSA105050100Dde I37510010075Dpn I410010075100Dpn IIUNRNRdd100NRDra I4757550100Dra III3 + BSA1007510025Drd I4255010100Eae I110010050100Eag I3102510010Ear I110010050100Eci I2 + BSA5010010050EcoN I410010075100EcoO109 I4 + BSA10010075100EcoR IUdd100100100100EcoR V3 + BSA507510050Fat I *SE-G101005050Fau I * 55CSE-B100251050Fnu4H I4102525100Fok I45010075100Fse I4 + BSA100750100Fsp I4107510100Hae II4 + BSA7510050100Hae III2501002575Hga I11007550100Hha I4 + BSA75100100100Hinc II3 + BSA75100100100Hind III2501001050Hinf I2751007575HinP1 I2100100100100Hpa I4255010100Hpa II11005010100Hph I4NR750100Hpy99 I4 + BSA10 10 0 100 Hpy188 I450 25 10 100 Hpy188 III4 + BSA100 100 10 100 HpyCH4III4100 50 25 100 HpyCH4IV1100 25 10 25 HpyCH4V450 50 25 100 I-Ceu I4 + BSA10100100I-Sce IU + BSA10505050Kas I2 + BSA751007575Kpn I1 + BSA10075050NextPage Mbo I375100100100Mbo II210010050100McrBC2 + BSA + GTP501001075Mfe I4755010100Mlu I3257510050Mly I4 + BSA505025100Mme I4 + SAMNRNRNR100Mnl I2 + BSA751005075Msc I4757575100Mse I2 + BSA751007575Msl I210010025100Msp I2751005050MspA1 I4 + BSA5010050100Mwo I 60CU107510075Nae I1100751075N.Alw I2251002550Nar I1100757575N.BbvC IA25010010100N.BbvC IB25010010100N.BstNB I 55CU0101010Nci I41002510100Nco I4100100100100Nde I47510075100NgoM IV41005010100Nhe I2 + BSA10010010100Nla III4 + BSA252525100Nla IV4 + BSA101010100Not I3 + BSA07510050Nru IU0107510Nsi IU25255010Nsp I2 + BSA1001000100Pac I1 + BSA1007510100PaeR7 I42510010100Pci I * SE-O257510050PflF I4 + BSA02525100PflM I3 + BSA010010050PI-Psp I 65CU + BSA0101010PI-Sce IU + BSA0000Ple I410010075100Pme I4 + BSA05010100Pml I1 + BSA10075075PpuM I40250100PshA I4 + BSA507510100Psi I *SE-B50100025PspG I 75C27510050100PspOM I *SE-Y2510050100Pst I3 + BSA757510050Pvu I3 + BSA107510010Pvu II2100100100100Rsa I110010050100Rsr II4257510100Sac I1 + BSA1005010100Sac II4257510100Sal IU + BSA00500Sap I475500100Sau3A IU + BSA1005010100Sau96 I450100100100Sbf I *SE-Y75500100Sca IU2575dd5025ScrF I4100100100100SexA I3 + BSA2525100100SfaN I307510025Sfc I4 + BSA755010100Sfi I 50C2 + BSA010010100Sfo I2251005050SgrA I41005010100Sma I 25C4000100Sml I 55C4 + BSA257525100SnaB I4 + BSA255025dd100Spe I2 + BSA751002575Sph I210010050100Ssp IU501005050Stu I210010050100Sty I3 + BSA257510010StyD4 I210100100100Swa I 25C3 + BSA101010010Taq I 65CU + BSA50757575Tfi I 65C3100100dd100100Tli I 75C3 + BSA252510010Tse I 65C37510010075Tsp45 I 65C1 + BSA10025075Tsp509 I 65C1100100100NRTspR I 65C4 + BSA255025100Tth111 I 65C11002525100Xba I2 + BSA01007575Xcm I2101005050Xho I2 + BSA75100100100Xma I4 + BSA25500100Xmn I2 + BSA10010075100Zra I *SE-B10010025100请您对这篇文章发表看法(0) (0) 到论坛中讨论 相关分子生物学类实验方法Cloning of small RNAs with 5 phosphate and 3 OH ends 2010-1-28 噬菌体DNA(48502bp)的限制性内切酶酶切片段 2009-12-30 转移至硝酸纤维素滤膜前后进行的染色 2009-12-30 质粒载体和外源中限制酶切位点的性质 2009-12-30 经乙二醛和二甲基亚砜变性处理后进行的RNA电泳 2009-12-30 外源片段未的性质 2009-12-30 用T4噬菌体多核苷酸激酶标记寡核苷酸探针 2009-12-30 将变性RNA转移至硝酸纤维素滤膜 2009-12-30 分子克隆常用技术 2009-12-30 用大肠杆菌DNA聚合酶IKlenow片段标记合成的寡核苷酸 2009-12-30 生物大分子浓缩、干燥及保存 2009-12-30 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